AffiCHECK® Escherichia coli (EPEC) DNA - PCR Qualitative Positive Control

SKU: AFG-CHK-0491

AffiCHECK® Escherichia coli (EPEC) DNA - PCR Qualitative Positive Control

AffiGEN
$893.00 AUD
$893.00 AUD
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AffiCHECK® Escherichia coli (EPEC) DNA - PCR Qualitative Positive Control

Product Datasheet - AFG-CHK-0491

PRODUCT DESCRIPTION:

AffiCHECK® Escherichia coli (EPEC) DNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Escherichia coli (EPEC) DNA. It's designed using modified and non-infectious bacteria to ensure stability, thereby contributing to accurate and reliable results in PCR applications.

INTENDED USE:

AffiCHECK® Escherichia coli (EPEC) DNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Escherichia coli (EPEC) nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.

PRODUCT FEATURES:

  • Specificity: Tailored for Escherichia coli (EPEC) DNA detection.
  • Formulation: Lyophilized for enhanced stability.
  • Convenience: Ready-to-use upon reconstitution.
  • Compatibility: Suitable for a wide range of PCR assays.

CHARACTERISTICS:

  • Cultivation & Extraction: Cultivated in MacConkey agar supplemented with sorbitol and extracted using a reliable genomic DNA method.
  • Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
  • Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.

PERFORMANCE CHARACTERISTICS:

  • Sensitivity: Optimized for high sensitivity in PCR detection.
  • Specificity: Exclusively detects Escherichia coli (EPEC) DNA.
  • Reproducibility: Consistent results across different test runs and batches.

PACK CONTENT :

Components Pack Size Note 
DNA Control Lyophilized Powder (1x104 to 2x104 Copies/µL) 1 Vial Quantified by Real-Time PCR.
Buffer 500 µL Water, DNase, RNase Free.

PRECAUTIONS:

  • For research use or diagnostic control purposes only.
  • Follow standard laboratory practices and use PPE.
  • Use Sterile Filter Tips to prevent contamination.
  • Handle infectious specimens with precautions.
  • Disinfect work surfaces.
  • Maintain separate working areas for testing.
  • Dispose of unused reagents and waste as per regulations.
  • May contain genetic material, handle with care.
  • No method ensures total absence of infectious agents.
  • Follow local waste disposal rules.
  • Dilute below 103 copies/µL immediately before use.
  • Avoid freezing for low-copy dilutions to prevent DNA degradation.

INSTRUCTIONS FOR USE:

  • Open the foil pouch of AffiCHECK® Escherichia coli (EPEC) DNA.
  • Centrifuge AffiCHECK® Escherichia coli (EPEC) DNA Lyophilized Powder at 1000 g for 1 minute.
  • Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
  • The concentration will be 1x104 to 2x104 Copies/µL once reconstituted.
  • Vortex for 1 min for complete dissolution and homogenization.

RECOMMENDED STORAGE:

  • Before Reconstitution: Store at 2-8ºC.
  • After Reconstitution: Store at -20ºC.
  • Shipping: No special instructions
  • Shelf Life: 12 months from production date.

ORDERING INFORMATION :

Catalog Number Description Size
AFG-CHK-0491 AffiCHECK® Escherichia coli (EPEC) DNA PCR Qualitative Positive Control 1 vial + 500 µL Buffer