SKU: AFG-VZ-N102-01

AffiZYME® T4 Polynucleotide Kinase

Vendor AffiGEN
Regular price $964.00 AUD
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AffiZYME® T4 Polynucleotide Kinase

AffiZYME® T4 Polynucleotide Kinase catalyzes the transfer of the y-phosphate from ATP to a 5'-hydroxy terminus (double-stranded or single-stranded DNA or RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also has 3'-phosphatase activity. which can hydrolyze 3'-phosphoryl groups from oligonucleotide 3'-phospahte termini, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates.

 Components:

Components AFG-VZ-N102-01 10,000 U
T4 Polynucleotide Kinase (10 U/µL) 1 mL
10X T4 PNK Buffer 2 x 1 mL

 

Reaction Buffer:

10X T4 PNK Buffer :

700 mM Tris-HCI pH 7.6 @ 25°C
100 mM MgCI2
50 mM DTT

 

Storage:

Store t -30 ~-15° and transport at≤0°C.