SKU: AFG-SNG-TR-015

AffiGEN® Transfect(TM) DNA & siRNA In Vitro 4000 Buffer (5X)

Vendor AffiGEN
Regular price €102,75 EUR
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AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X)

Product Description

AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) has been specifically designed to complement the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit (Cat# AFG-SNG-TR-11AFG-SNG-TR-12), ensuring maximal DNA transfection efficiency and optimal gene silencing outcomes. Prior to use, it is necessary to dilute the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) by adding 4 parts of ddH2O. The resulting AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer working solution is employed to dilute DNA, siRNA, and the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Reagent to form the most effective transfection complex.

Package

Each vial of AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) contains 8.0 mL of the solution, which, when appropriately diluted, yields a 40 mL working solution (1X). This quantity is sufficient for approximately 600 transfections in a 24-well plate.

Storage

Store AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) at room temperature (RT). When stored under proper conditions, the product remains stable for 12 months or longer.

 

DNA In Vitro Transfection Reagents Specifications

Product Type Toxicity Efficiency Serum Tolerance Delivery Method Transfection Type Package Shelf Life
AffiGEN® TransfectTM 1000 - Mammalian Cell DNA High High Low Calcium Phosphate Precipitation Non-liposomal 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA In Vitro 2000 V1 DNA Low High High Liposomal Cationic Liposomal 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA In Vitro 2000 V2 DNA Moderate Moderate Moderate Liposomal Cationic Liposomal 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA In Vitro 2000 Plus DNA Moderate High Moderate Liposomal Cationic Liposomal 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA In Vitro 3000 DNA Low High High Liposomal Cationic Liposomal 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit DNA & siRNA Low Moderate High Polymer-based N/A 1 mL, 5X 1 mL 2 years at 4°C
AffiGEN® TransfectTM DNA In Vitro 5000 DNA Low High High Liposomal Cationic Liposomal 1 mL, 5X 1 mL 2 years at 4°C

 

DNA In Vitro Transfection Reagents Application Guide

Product Target Organisms Applications Cell Types Suitable for Additional Notes
AffiGEN® TransfectTM 1000 - Mammalian Cell Wide range, including hard-to-transfect mammalian cells Gene expression, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Virus, antibody and protein production No serum required, simple protocol, can be cytotoxic in some cell lines
AffiGEN® TransfectTM DNA In Vitro 2000 V1 Mammalian cells (HEK293, CHO, COS, etc.) Gene expression, virus production, antibody production HEK293, CHO, COS, stem cells Sensitive Cells & maximum cell viability High efficiency in stem cells, low cytotoxicity
AffiGEN® TransfectTM DNA In Vitro 2000 V2 Wide range, including hard-to-transfect mammalian cells Gene expression, virus production, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Common cell types Efficient for virus production, compatible with wide range of media
AffiGEN® TransfectTM DNA In Vitro 2000 Plus Wide range, including hard-to-transfect mammalian cells Gene expression, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Very long DNAs Improved efficiency and viability compared to AffiGEN® TransfectTM DNA In Vitro 2000 V2, reduced cytotoxicity
AffiGEN® TransfectTM DNA In Vitro 3000 Wide range, including hard-to-transfect mammalian cells Gene expression, virus production, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Lentivirus, antibody & protein production High efficiency with serum/antibiotics, compatible with wide range of media
AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit Wide range, including hard-to-transfect mammalian cells Gene expression, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Best transfection with least toxicity Biodegradable, compatible with wide range of media, low cytotoxicity
AffiGEN® TransfectTM DNA In Vitro 5000 Wide range, including hard-to-transfect mammalian cells Gene expression, virus production, protein production Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) Common mammalian cells High in-vivo delivery efficiency, compatible with wide range of media