AffiGEN® TransfectTM DNA In Vitro 2000 V2
Product Decription
AffiGEN® TransfectTM DNA In Vitro 2000 V2 represents an enhanced iteration of the AffiGEN® TransfectTM DNA In Vitro 2000 V1. Incorporating innovative chemistry, AffiGEN® TransfectTM DNA In Vitro 2000 V2 unleashes a greater number of DNA condensing groups (100% compared to 80% in the previous version), resulting in a remarkable 3~4 fold increase in transfection efficiency, all while preserving cell viability. Each unit of AffiGEN® TransfectTM DNA In Vitro 2000 V2 reagent, with a volume of 1.0 ml, is sufficient for more than 600 transfections in 24-well plates or more than 300 transfections in 6-well plates.
Features
- Achieves 3~4 times higher efficiency, tailored to the specific cell type.
- The preferred choice for challenging-to-transfect cells.
- Demonstrates increased efficiency even in the presence of serum and antibiotics for most cell types.
- Yields exceptionally high levels of recombinant protein production.
Storage Condition
Store AffiGEN® TransfectTM DNA In Vitro 2000 V2 at 4°C. When stored appropriately, the product maintains stability for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
| AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
| Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
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| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
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| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
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| AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
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| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
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| AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
|