AffiCHECK® Mycoplasma pneumoniae DNA PCR Quantitative Positive Control
Product Datasheet - AFG-CHK-0532
PRODUCT DESCRIPTION:
AffiCHECK® Mycoplasma pneumoniae DNA PCR Quantitative Positive Control is a high-quality, reliable reference standard containing known concentrations of Mycoplasma pneumoniae DNA. Formulated with modified, non-infectious bacterial material, it ensures stability and safety while supporting accurate and consistent results in quantitative PCR (qPCR) applications.
INTENDED USE:
AffiCHECK® Mycoplasma pneumoniae DNA PCR Quantitative Positive Control serves as a comprehensive process control for evaluating the performance of nucleic acid amplification tests (NAATs) used to detect and quantify Mycoplasma pneumoniae DNA. It is essential for validating the accuracy and reliability of PCR assays in clinical, research, and diagnostic settings.
PRODUCT FEATURES:
- Specificity: Precisely formulated for the detection of Mycoplasma pneumoniae DNA.
- Formulation: Lyophilized to enhance long-term stability and ease of storage.
- Convenience: Ready-to-use upon reconstitution.
- Compatibility: Designed to perform reliably across a broad range of PCR and qPCR assay platforms.
CHARACTERISTICS:
- Cultivation & Extraction: Cultivated in Eaton's agar and extracted using a reliable genomic DNA method.
- Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
- Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.
PERFORMANCE CHARACTERISTICS:
- Sensitivity: Optimized for high sensitivity in PCR detection.
- Specificity: Exclusively detects Mycoplasma pneumoniae DNA.
- Reproducibility: Consistent results across different test runs and batches.
PACK CONTENT :
| Components | Pack Size | Note |
| DNA Control Lyophilized Powder (1x104 to 2x104 Copies/µL) | 1 Vial | Quantified by Real-Time PCR. |
| Buffer | 500 µL | Water, DNase, RNase Free. |
PRECAUTIONS:
- For research use or diagnostic control purposes only.
- Follow standard laboratory practices and use PPE.
- Use Sterile Filter Tips to prevent contamination.
- Handle infectious specimens with precautions.
- Disinfect work surfaces.
- Maintain separate working areas for testing.
- Dispose of unused reagents and waste as per regulations.
- May contain genetic material, handle with care.
- No method ensures total absence of infectious agents.
- Follow local waste disposal rules.
- Dilute below 103 copies/µL immediately before use.
- Avoid freezing for low-copy dilutions to prevent DNA degradation.
INSTRUCTIONS FOR USE:
- Open the foil pouch of AffiCHECK® Mycoplasma pneumoniae DNA.
- Centrifuge AffiCHECK® Mycoplasma pneumoniae DNA Lyophilized Powder at 1000 g for 1 minute.
- Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
- The concentration will be 1x104 to 2x104 Copies/µL once reconstituted.
- Vortex for 1 min for complete dissolution and homogenization.
RECOMMENDED STORAGE:
- Before Reconstitution: Store at 2-8ºC.
- After Reconstitution: Store at -20ºC.
- Shipping: No special instructions
- Shelf Life: 12 months from production date.
ORDERING INFORMATION :
| Catalog Number | Description | Size |
| AFG-CHK-0532 | AffiCHECK® Mycoplasma pneumoniae DNA PCR Quantitative Positive Control | 1 vial + 500 µL Buffer |