AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit
Product Description
AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit, harnessed from our innovative and proprietary lipid-conjugation technology, incorporating novel fluorinated cationic lipids, showcases substantial advantages over other lipid-based transfection reagents in the market. AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit represents the most potent yet gentle gene delivery solution, aptly suited for a diverse array of applications, including the transfection of plasmid DNA and/or siRNA into most mammalian cell types. When compared to leading products in the market, AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit proves to be not only more cost-effective but consistently delivers superior transfection efficiency with reduced cytotoxicity.
Kit content
- AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit Reagent, 1.0 ml, sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).
- AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5x), 8.0 mL, to prepare a 40 mL working solution (1x).
Features
- Versatile: Supports DNA transfection, siRNA transfection, and DNA/siRNA co-transfection.
- Powerful: Achieves exceptional efficiency with adherent cells.
- Remarkably low toxicity: Enhances cell viability post-transfection.
- Economical: Enables more transfections with less reagent.
- User-friendly: Conducted as a one-tube reaction and compatible with serum/antibiotics.
- Ideal for high-throughput applications.
Storage
Store AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit Reagent at 4°C and AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5x) at room temperature (RT). When stored correctly, the product maintains stability for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
| AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
| Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
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| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
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| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
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| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
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| AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
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| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
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| AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
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