Overview
AffiGEN® DH5α Fast Chemically Competent Cell from AffiGEN is a competent cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 108 cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification and can ensure the stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA. F-DH5α competent cells were prepared by special technology, without heat shock at 42℃ or incubated at 37℃, and only 10 minutes to complete a transformation. DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ (lacZYA-arg F) U169 endA1 recA1 hsdR17 (rk-, mk+) supE44λ- thi -1 gyrA96 relA1phoA
Shipping Condition: Dry ice
Product Format: Liquid