SKU: AFG-E8348

AffiELISA® Human TNFaIP3 Kit: High-Resolution Tumor Necrosis Factor Alpha Induced Protein 3 ELISA Detection

Verkäuferin AffiGEN
Regulärer Preis €389,00 EUR
Verkaufspreis €389,00 EUR Regulärer Preis
Verkauf Ausverkauft
Stückpreis
/pro 
Versand An der Kasse berechnet.
Size:
Text vorbestellen
The AffiELISA® Human TNFaIP3 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) in the samples can be determined.