AffiBEADS® Streptavidin Magnetic Beads
AffiBEADS® Streptavidin Magnetic Beads are designed for high-efficiency binding with biotinylated molecules, facilitating various applications such as immunoprecipitation, protein purification, and cell isolation. These beads exhibit strong magnetic properties, ensuring rapid and efficient magnetic separation.
AffiBEADS® Streptavidin Magnetic Beads Features
- High Binding Capacity: Efficient binding with biotinylated molecules.
- Versatile Applications: Ideal for immunoprecipitation, protein purification, and cell isolation.
- Rapid Separation: Strong magnetic properties for quick and efficient separation.
AffiBEADS® Streptavidin Magnetic Beads Specifications
- Bead Diameter: 2.8 µm.
- Binding Capacity: High affinity and specificity for biotin.
- Storage Conditions: Store at 2-8°C, avoid freezing.
AffiBEADS® Streptavidin Magnetic Beads Applications
- Immunoprecipitation
- Protein Purification
- Cell Isolation
AffiBEADS® Streptavidin Magnetic Beads Protocol
Prepare Beads:
- Reconstitute the beads in ultrapure water to 1 mg/mL.
- Wash the beads three times.
Bind Proteins:
- Dilute the biotinylated protein or antibodies with Assay Buffer.
- Add to the beads and incubate for 60 minutes at room temperature on a rotary mixer.
Final Wash:
- Wash the beads four times.
- Add Assay Buffer to the beads for the next experiment.
Note:
Streptavidin has an extremely high affinity for biotin, making the binding nearly irreversible. These beads are pre-coupled with Streptavidin and blocked with BSA, ready for immediate use.