AffiNGS® Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat)
AffiNGS® Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) is a kit for removing Globin mRNA and rRNA from total RNA in blood samples. This kit is compatible with 0.01 - 1 µg blood total RNA. The total RNA sample undergoes Globin mRNA and rRNA hybridize with probe, RNase H digestion, DNase I digestion, etc. Finally, Globin mRNA and rRNA (including cytoplasmic rRNA and mitochondrial rRNA) are removed from total RNA, leaving other mRNA and non-coding RNA, which can be used for the analysis of non-coding RNA such an LncRNA. This kit is compatible with partially degraded RNA samples, and the obtained products are suitable for RNA library construction and the other experiments.
Components:
| Components | AFG-M-0469 (24 rxns) | AFG-M-0470 (96rxns) |
| Globin and rRNAA Probe (H/M/R) | 24 µl | 96µl |
| Probe Buffer | 72 µl | 288 µl |
| Rnase H Buffer 2 | 72 µl | 288 µl |
| Rnase H Mix | 48 µl | 192 µl |
| Dnase I Buffer | 696 µl | 4 x 696 µl |
| Dnase I | 24 µl | 96 µl |
|
Nuclease-free ddH2O |
1 ml | 4 x 1 ml |
Storage:
Store at -30 ~ -15° and transport at ≤0°C.