AffiZOL® Lysis Reagent
(Phenol-Guanidine Solution for RNA, DNA & Protein Extraction)
Product Description
AffiZOL® Lysis Reagent is a monophasic phenol and guanidine isothiocyanate solution optimized for the rapid extraction of high-quality total RNA, with optional recovery of DNA and proteins from the same sample. Its robust performance across diverse sample types makes it ideal for high-throughput RNA workflows in molecular biology, diagnostics, and functional genomics.
AffiZOL® effectively disrupts cells, dissolves cell components, and simultaneously inactivates RNases and DNases—ensuring RNA integrity during the entire extraction process.
Features & Benefits
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🔬 Three-in-One Extraction – Isolate RNA, DNA, and proteins from a single sample
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⚡ Fast & Convenient Protocol – Minimal hands-on time with consistent yields
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🧪 Superior RNA Integrity – Ideal for downstream applications like RT-qPCR, RNA-seq, and microarrays
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🌱 Broad Sample Compatibility – Efficient on tissues, cultured cells, blood, bacteria, and plant material
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🧊 Stable & Ready-to-Use – Long shelf life when stored at 2–8°C
Kit Contents
| Catalog No. | Format | Reactions | Kit Components |
|---|---|---|---|
| AFG-M-0001 | Standard Kit | 200 Reactions | 100 mL AffiZOL® Reagent 40 mL Dilution Buffer |
| AFG-M-0002 | Large Kit | 400 Reactions | 200 mL AffiZOL® Reagent 80 mL Dilution Buffer |
Applications
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Total RNA extraction for:
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RT-PCR / qPCR
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RNA-Seq
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Northern blotting
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Gene expression analysis
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Genomic DNA and protein co-extraction
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Sample preparation for multi-omics workflows
Protocol Overview
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Homogenize sample in AffiZOL® Reagent
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Add chloroform and centrifuge to separate phases
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Recover RNA from aqueous phase
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Optionally extract DNA and proteins from interphase and organic phase
Storage & Stability
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Store at 2–8°C, protected from light
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Shelf life: 12 months