AffiNGS® DNA Damage Repair Kit

SKU: AFG-BCL-000001

AffiNGS® DNA Damage Repair Kit

$450.00 USD
$450.00 USD
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AffiNGS® DNA Damage Repair Kit

Optimized enzyme mix for repairing damaged DNA, especially from FFPE (formalin-fixed paraffin-embedded) samples.

Key Benefits:

  • High repair efficiency for FFPE-derived DNA.
  • Reaction buffer compatible with end repair/adenylation workflows.
  • No further purification needed after using thermosensitive proteinase K.
  • Direct integration into library construction workflows.

Components:

Components  AFG-BCL-000001
(24 rxns)		  AFG-BCL-000002
(96 rxns)
Damaged DNA Repair Enzymes 72 μL 288 μL

 

Storage:

  • Storage: −20°C to −10°C.
  • Shipping: Use dry ice or combination of dry ice and ice packs, maintain between −40°C to −20°C.

Protocol:

Important Notes:

  • Use only with severely damaged DNA such as FFPE samples.
  • Not compatible with Cell-free DNA (cfDNA) – may cause random fragmentation.
  • Not compatible with Tissue or high-quality genomic DNA – unnecessary and may not be effective.

1. Recommended DNA Input Amount:

Sample Type Suggested Input DNA (mass) Notes
FFPE DNA 100–500 ng
 Recommended range

200–300 ng Common working range

Up to 1 µg Higher input gives better libraries (if available)

<100 ng Possible for rare samples; expect lower yield

 

2. Reaction Setup (on ice):

Compatible with ultrasonic-based fragmentation protocols.

 Reagent Volume Notes
Fragmented FFPE DNA X μL Adjust to desired input amount (see above)
End Repair Buffer (from your kit) Y μL As per your library kit's recommendation
End Repair Enzyme Mix Z μL As per your library kit's recommendation
Damaged DNA Repair Enzymes 3 μL Enhances repair of damaged FFPE DNA
Nuclease-free Water Up to final volume (e.g., 60 μL) Adjust to total reaction volume

 

3. Mixing & Incubation:

  • Mix thoroughly by pipetting.
  • Briefly centrifuge.
  • Incubate using a PCR thermocycler (lid temperature: 105°C):
 Temperature Duration
30°C 30 min
65°C 30 min
4°C Hold

 

4. Next Steps:

Proceed directly to:

  • Adapter ligation
  • Purification
  • Library amplification

Follow your library prep kit's instructions for all subsequent steps.