Overview
Chloramphenicol, also known as Chloromycetin, Chlornitromycin, or Levomycetin, is a broad-spectrum antibiotic. USP Grade Chloramphenicol is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol resistance genes. Chloramphenicol is a broad-spectrum antibiotic that inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step and prevents attachment of aminoacyl tRNA to the ribosome. It is usually bacteriostatic and effective against a wide range of gram-positive and gram-negative organisms. In addition, Chloramphenicol exhibits anti-rickettsial activity and is active against chlamydias of the psittacosis-lymphogranuloma group. Chloramphenicol acetyltransferase (CAT) catalyzes the acetylation of chloramphenicol, causing resistance to chloramphenicol. Chloramphenicol is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol resistance genes.
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