AffiVET® Canine Parvovirus CPV & Canine Coronavirus CCV Antigen Rapid Test Kit

AffiVET® Canine Parvovirus CPV & Canine Coronavirus CCV Antigen Rapid Test Kit

What is canine parvovirus?

Canine parvovirus is a highly contagious virus that can affect all dogs, but unvaccinated dogs and puppies younger than four months old are the most at risk. Dogs that are ill from canine parvovirus infection are often said to have "parvo." The virus affects dogs' gastrointestinal tracts and is spread by direct dog-to-dog contact and contact with contaminated feces (stool), environments, or people. 

What are canine parvovirus symptoms?

Some of the signs of parvovirus include lethargy; loss of appetite; abdominal pain and bloating; fever or low body temperature (hypothermia); vomiting; and severe, often bloody, diarrhea. Persistent vomiting and diarrhea can cause rapid dehydration, and damage to the intestines and immune system can cause septic shock.

One-step Canine Parvovirus, Canine Coronavirus and Giardia Antigen Test : 

                                                                         For veterinary diagnostic use only

AffiVET® Rapid CPV/CCV/Giardia Ag Test Kit : 

Principles : 

The AffiVET Rapid CPV/CCV/Giardia Ag Test Kit: is a chromatographic immunoassay for
the qualitative detection of canine parvovirus, canine corona virus, and giardia antigen
in canine feces.
The AffiVET Rapid CPV/CCV/Giardia Ag Test Kit: has two letters which are test(“T”) line
and control(“C”) line on the surface of device. Test line and control line in the result
window are not visible before applying any samples. The control line is a reference line
which indicates the test is performing properly. The control line has to appear every
time when the test has performed. If the target antigens are present in sample, a purple
test line would appear in the result window.
The highly selective antibodies to target antigen are used as each capture and detector
in the assay. These are capable of detecting canine parvovirus, canine corona virus, and
giardia antigen in sample with high accuracy.

Materials provided : 

Materials required, but not provided : 

1)Timer

Precautions : 

1. The test kit is for canine use only. Do not use for other animals.
2. The test device is sensitive to humidity as well as heat. Perform the test immediately after removing the test device from the foil pouch.
3. Do not reuse the test components.
4. Apply the sample using dropper vertically.
5. Do not touch the membrane in the result window of test device.
6. Do not use the test kit beyond the stated expiration date marked on the package label.
7. Do not use the test kit if the pouch is damaged or the seal is broken.
8. Do not mix components from different lot numbers because the components in this kit have been quality control tested as standard batch unit.
9. All samples should be handled as being potentially infectious. Wear protective gloves while handling samples. Wash hands thoroughly afterwards.
10. Decontaminate and dispose of all samples, used kits and potentially contaminated materials safely in accordance with national and local regulations.
11. This product is designed for primary antigen screening and is not suitable for use for verifying the progress of treatment and determining the cure. 

Storage and Stability : 

1. Store the test kit at 2~30°C. DO NOT FREEZE.
2. Do not store the test kit in the direct sunlight.
3. The test kit is stable within the expiration date that marked on the package label. 

Collection and Preparation of Sample : 

1. The canine feces should be used as a sample for this test.
2. The samples should be tested immediately after collection.
3. If samples are not tested immediately, they should be stored at 2~8°C for 24 hours. For longer storage, freeze at -20°C or below.
4. The amount of fecal swab may affect the results. It is required to follow the swab amount of feces as shown in the picture on the below. Excessive fecal amount may induce a false positive result and slow migration.

1. Not enough
2. Good
3. Good
4. Too Much

Procedure of the Test : 

1. All reagents and samples must be at room temperature (15~30°C) before use.
2. Collect the samples for CPV/CCV Ag and Giardia Ag Test using 2 swabs.
3. Insert the swab into the assay diluents tube(P) for CPV/CCV Ag Test and assay diluents tube(G) for Giardia Ag Test. Mix the swab until the sample has been dissolved into the assay diluents(Approximately 10 sec.).                                      Pre-wet swab with a saline solution P G • CPV/CCV Ag (P) • Giardia Ag (G)
4. Wait for 1 minute to settle down the large particles
5. Remove the test device from the foil pouch, and place it on a flat and dry surface 
6. Using the disposable dropper, take the supernatant sample in the tube.
7. Add 4 drops of each mixed sample into each sample hole, drop by drop vertically                                                                                                         NOTE: Use different disposable droppers for assay diluents (P) and assay diluents (G)
8. Start the timer. The sample will flow across the result window. If it does not appear after 1 minute, add one more drop of mixed sample to the sample hole.
9. Interpret test results at 5~10 minutes. Do not read the result after 20 minutes

Interpretation of the Result : 

1. Negative result : Only Control (C) Line Appears In The Result Window

• CPV Ag : Dark Control Line (C) and No Test Line (T)
• CCV Ag : Dark Control Line (C) and No Test Line (T)
• Giardia Ag : Dark Control Line (C) and No Test Line (T)

2. Positive result : 

Test (T) line and control (C) line within the result window indicate the presence of CPV, CCV and/or Giardia antigen 

CPV Ag Positive : 

• CPV Ag : Dark Control Line (C) and Dark Test Line (T)
• CCV Ag : Dark Control Line (C) and No Test Line (T)
• Giardia Ag : Dark Control Line (C) and No Test Line (T)

CCV Ag Positive : 

• CPV Ag : Dark Control Line (C) and Dark Test Line (T)
• CCV Ag : Dark Control Line (C) and Dark Test Line (T)
• Giardia Ag : Dark Control Line (C) and No Test Line (T)

 Invalid Result : 

If the control(C) line does not appear, the result might be considered invalid. The sample should be re-tested.

• CPV Ag : No Control Line (C) and Dark Test Line (T)
• CCV Ag : No Control Line (C) and Dark Test Line (T)
• Giardia Ag : No Control Line (C) and Dark Test Line (T)

• CPV Ag : No Control Line (C) and No Test Line (T)
• CCV Ag : No Control Line (C) and No Test Line (T)
• Giardia Ag : No Control Line (C) and No Test Line (T)

Limitations of the test : 

1.  Although the Anigen Rapid CPV/CCV/Giardia Ag Test Kit is very accurate in detecting canine parvovirus, canine corona virus, and giardia antigen, a low incidence of false results can be occurred. Other clinically or laboratory tests might be required if questionable results are obtained. As other diagnostic tests, a definitive clinical diagnosis should not be based on the result of a single test, but should be diagnosed by the veterinarian after all clinical and laboratory findings have been evaluated.
2. The reading window may show a light pink background coloration; this will not affect the accuracy of the results.
3. BioNote and its distributors cannot be held responsible for the consequences of misuse or misinterpretation of the results given by the test.

AffiVET®Rapid : 

General Information : 

Purpose : Detection of Canine Parvovirus, Coronavirus and Giardia antigen
Principle : Immunochromatographic assay
Specimen : Canine feces

Materials provided :
• Anigen Rapid CPV/CCV/Giardia test device
• Assay diluents tube for CPV/CCV Ag (P)
• Assay diluents tube for Giardia Ag (G)
• Disposable swab
• Disposable dropper

Special Features :
• Simultaneous detection of CPV, CCV and Giardia
• One-step testing procedure : Quick & Accurate
• High sensitivity and specificity 

Canine : 

Test Procedure : 

[ Proper sample amount collected by a swab] 

1.  Collect a sample from the canine faces by using the swab 
2. Insert the swab into the sample collection tube and stir the swab at least 10 times 
3. Discard the swab while squeezing the swab against the wall of the tube 
4. Wait until large particles to sink and take the supernatant by using the disosable dropper provided 
5. Add 4 drops into the sample hole on the test device.

Interpretation : 

Ordering Information :