AffiVET® Canine Heartworm CHW Antigen Rapid Test Kit

Test Principal:

The AffiVET® Rapid CHW Antigen Test Kit is a chromatographic immunoassay designed for the qualitative detection of Canine Dirofilaria immitis antigen in canine serum,plasma, or whole blood.

This kit features two distinct lines on the device's surface: the test (T) line and the control (C) line. Initially invisible in the result window, these lines become visible upon the application of a sample. The control line serves as a crucial reference, confirming the proper execution of the test and is expected to appear with every test conducted.

When IgG antibodies specific to canine heartworm antigens are present in the sample, a purple test line will appear in the result window.

The highly selective antibodies to Canine Dirofilaria immitis antigens are utilized as both capture and detector components in the assay, enabling the accurate detection of IgG antibodies to Canine Dirofilaria immitis antigen in samples with high precision.

Material Required:

Storage & Stability:

• Store the test kit at 2~30°C. DO NOT FREEZE.
• Do not store the test kit in the direct sunlight.
• The test kit is stable within the expiration date marked on the package label.
  

Sample collection & Preparation:

• Collect blood using a disposable syringe.
• Place the collected blood sample into the anticoagulant tube.
• Secure the cap on the anticoagulant tube and invert it five times to mix the blood and EDTA.
• Test the collected blood either immediately or within 4 hours at room temperature. After testing, store it between 2~8°C for up to 24 hours.
• Samples with significant hemolysis may impact the test outcome.

• Collect blood in a tube without anticoagulants (e.g., heparin, EDTA, sodium citrate).
• Let it settle for 30 minutes to clot, then centrifuge to obtain serum.
• Refrigerate the collected serum at 2-8°C for up to 2 weeks. For longer storage, freeze it at -20°C or below for up to 1 year.

• Collect blood in a tube with anticoagulants, then centrifuge to obtain plasma.
• Refrigerate the collected plasma at 2-8°C for up to 2 weeks. For longer storage, freeze it at -20°C or below for up to 1 year.

Test Procedure:

1. All reagents and samples must be at room temperature (15~30°C) for 15~30 minutes before use.
2. Remove the test device from the foil pouch, and place it on a flat and dry surface.
3. Using a disposable dropper, add 2 drops of sample into the sample hole vertically.
4. Start the timer. The sample will flow across the result window. If it does not appear after 1 minute, add one more drop mixed sample to the sample hole.
5. Interpret test results at 5-10 minutes. Do not interpret after 20 minutes.

Result Interpretation:

Negative result

This means that there is no CHW Antigens in the sample or the level is lower than the test detection limit.

Positive result:

This confirms the presence of the CHW Antigens in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

• Use this test kit exclusively for canine samples; do not use it for other animals.
  
• The test device is sensitive to humidity and heat, so perform the test immediately after removing it from the foil pouch.
  
• Do not reuse any test components to ensure accurate results.
  
• Apply the sample using a disposable dropper in a vertical manner.
  
• Avoid touching the membrane in the result window of the test device.
  
• Do not use the test kit beyond its expiration date indicated on the package label.
  
• Do not use the test kit if the pouch is damaged or if the seal is broken.
  
• Refrain from mixing components from different lot numbers, as each kit undergoes quality control testing as a standard batch unit.
  
• Treat all samples as potentially infectious and wear protective gloves during handling. Thoroughly wash hands afterward.
  
• Dispose of all samples, reaction kits, and potentially contaminated materials safely in accordance with national and local regulations.
  
• Strictly follow the test procedures to minimize false or invalid test results due to improper administration of the product usage or doses.