AffiVET® Bovine TB-Feron Antigen ELISA Kit
Species: Ruminant
Disease or Pathogen: Tuberculosis
Target: Antibody
Test Type: Elisa Kit
Gamma interferon assay for Mycobacterium bovis
Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis.
The infection is often subclinical; even when present, clinical signs are not specifically distinctive of this disease and might include weakness, anorexia, emaciation, enlargement of lymphnodes, and cough. Routine screening can be time and labor intensive. AffiGen TB-Feron ELISA Plus is an Sandwich Enzyme Linked Immunosorbent Assay for the qualitative detection of interferon gamma (IFN-γ). IFN-γ assay is based on the fact that an animal has blood lymphocytes which can memorize stimulating antigen immunologically when it is stimulated by exogenous or endogenous antigens. When an antigen is added to the blood from a primed animal within a tube, antigen specific effector/memory T cell are rapidly re-stimulated to produce IFN-γ, the cytokine, which is used as a specific marker in cell-mediated immune response (recall response). It is an alternative test method of intradermal skin test (PPD) because of its easy procedure and better sensitivity compared to the skin test.
Indications
- Qualitative detection of interferon gamma in bovine plasma
- Diagnosis for government policies on slaughter
- First step for massive screening instead of PPD in a shorthanded situation
- Combine with ELISA or Rapid test in eradication system
Special Features
- Much more reliable than PPD skin test
- OIE standard for bTB diagnosis
- Good correlation with other IFN-γ assay
- Provide antigens for sensitization
- Specimen : Stimulated plasma
- Assay time : 90 minutes
- Concordance rate: 95.9% vs. “B” commercial ELISA