AffiGEN® Hyperactive pA-MNase for CUT&RUN

AffiGEN® Hyperactive pA-MNase for CUT&RUN

AffiGEN® Hyperactive pA-MNase for CUT&RUN fuses Protein A and Micrococcal nuclease (MNase) to form a novel fusion enzyme with their dual activities, and it is specifically for the CUT&RUN technology in protein-genome interaction research. CUT&RUN technology greatly simplifies the operation process of traditional ChIP-seq, only taking 1 - 1.5 days to construct the next generation sequencing library from cells. Moreover, it has low cell input, high signal-to-noise ratio, good reproducibility, and is widely applied to research including early embryonic development, stem cells, tumors, and epigenetics.

Storage Conditions:

Store at -30 ~ -15°C and transport at ≤0°C.

Applications:

This product is specially applicable to CUT&RUN technology for protein gene interaction research.

Source:

A recombinant E. coli strain with the MNase gene from Micrococcus was cloned.

Unit Definition:

At 25°C, the amount of enzyme required for highly polymerized DNA to increase the absorbance at 260 nm by 0.001/min/ml is defined as an active unit (U).

Notes:

For research use only. Not for use in diagnostic procedures.