{"title":"TOPO Cloning","description":"","products":[{"product_id":"afficlone-5-min-ta-blunt-zero-cloning-kit","title":"AffiCLONE® 5 min TA\/Blunt-Zero Cloning Kit","description":"\u003cp\u003e5 min TA\/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and a blunt end factor. Combining with the optimal buffer, the second generation of Topoisomerase provides a highly efficient, 5 min, one-step cloning strategy at room temperature. This product using a vector containing the suicide gene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5 min TA\/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt is suitable for ligation of blunt ended and A-Tailed PCR products\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures,\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents\u003c\/td\u003e\n\u003ctd\u003e25 Reactions\u003c\/td\u003e\n\u003ctd\u003e50 Reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5 × TA\/Blunt-Zero Cloning Mixa\u003c\/td\u003e\n\u003ctd\u003e25 μL\u003c\/td\u003e\n\u003ctd\u003e2 X \u003cspan data-mce-fragment=\"1\"\u003e25 μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500 bp Control insert (20 ng\/μl)\u003c\/td\u003e\n\u003ctd\u003e 5 μL\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eM13 Primer Mix (10 μM)b\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e400 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"AffiGEN","offers":[{"title":"25 Reactions (5 µL\/Reaction)","offer_id":46663597523275,"sku":"AFG-M-0157","price":201.0,"currency_code":"USD","in_stock":true},{"title":"50 Reactions (5 µL\/Reaction)","offer_id":46663597556043,"sku":"AFG-M-0158","price":362.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE.png?v=1741089619"},{"product_id":"afficlone-5-min-topo-blunt-cloning-kit","title":"AffiCLONE® 5 min TOPO-Blunt Cloning Kit","description":"","brand":"AffiGEN","offers":[{"title":"20 Reactions (2 µL\/Reaction)","offer_id":46663597621579,"sku":"AFG-M-0379","price":163.0,"currency_code":"USD","in_stock":true},{"title":"40 Reactions (2 µL\/Reaction)","offer_id":46663597654347,"sku":"AFG-M-0380","price":290.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_7020c55b-9095-4895-b56a-818311789e4a.png?v=1741089618"},{"product_id":"afficlone-ultra-universal-topo-cloning-kit","title":"AffiCLONE® Ultra-Universal TOPO Cloning Kit","description":"\u003cp\u003eAffiCLONE® Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt is applicable for the ligation of blunt-end or A-tailed PCR products.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003cbr\u003eStore at temperatures between -30℃ and -15℃, and ensure transportation at ≤0℃. After thawing, they can be stored at 4℃ for up to three months, maintaining their quality and efficacy.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"20 Reactions (10 μL\/Reaction)","offer_id":46663597687115,"sku":"AFG-M-0159","price":196.0,"currency_code":"USD","in_stock":true},{"title":"40 Reactions (10 μL\/Reaction)","offer_id":46663597719883,"sku":"AFG-M-0160","price":348.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_b753857a-0b1b-4d08-aed0-491a5112265b.png?v=1741089617"}],"url":"https:\/\/affigen.com\/pl\/collections\/topo-cloning.oembed","provider":"AffiGEN","version":"1.0","type":"link"}