{"title":"DNA Library Preparation Module","description":"","products":[{"product_id":"affi-circularization-kit-for-mgi","title":"AffiNGS® Circularization Kit For MGI","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® Circularization Kit For MGI\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThe AffiNGS® Circularization Kit for MGI\u003c\/strong\u003e is a kit optimized for the high-throughput sequencing platform of MGI. This kit can convert PCR products with adapters to single-stranded circularization DNA libraries dedicated to MGI high-throughput sequencer. All reagents provided in the kit have undergone rigorous quality control and functional validation to ensure the optimal stability and repeatability of library preparation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"119\" style=\"text-align: left;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"131\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003e AFG-M-0398\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (16 rxns)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd width=\"141\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003e AFG-M-0399\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (48 rxns)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSplint Oligo\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e160 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e480 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRapid Splint Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e240 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e720 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRapid DNA Ligase\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e80 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e240 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e128 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e384 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Enzyme\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e32 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: right;\"\u003e96 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30 ~ -15℃ and transport at ≤0℃.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"16 Reactions","offer_id":46663619838283,"sku":"AFG-M-0398","price":348.0,"currency_code":"USD","in_stock":true},{"title":"48 Reactions","offer_id":46663619871051,"sku":"AFG-M-0399","price":938.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_9423fe95-4584-4bfd-a1da-4bbfef0a0e2c.png?v=1741089528"},{"product_id":"affi-hifi-universal-amplification-mix-for-mgi-si","title":"AffiNGS® HiFi Universal Amplification Mix for MGI (SI)","description":"","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":46663619903819,"sku":"AFG-M-0400","price":77.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":46663619936587,"sku":"AFG-M-0401","price":268.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_8fae4105-b22f-47f3-92e7-2e0e6ef8b902.png?v=1741089528"},{"product_id":"affings-universal-end-preparation-module-for-illumina-v2","title":"AffiNGS® Universal End preparation Module for Illumina V2","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® Universal End preparation Module for Illumina V2\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiNGS® Universal End preparation Module for Illumina V2\u003c\/strong\u003e is a directional optimized End Repair module for Illumina high-throughput sequencing library construction. The end of 100 pg - 1 μg fragment DNA can be repaired efficiently with this kit. The 5' end of the repair product contains phosphate group and the 3' end has dA protrusion. Compared with the conventional library construction method, Universal End preparation Module for Illumina V2 adopts one-step reaction process, which combines the End Repair and dA tail steps, and meanwhile reduces the reaction time of each step, thus significantly reducing the minimum requirement of initial DNA template and shortening the experiment time. The reagent is in the form of Mix, which makes pipetting operation more convenient and compatible with automatic library construction equipment. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library construction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Components:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"196\" style=\"text-align: left;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"114\"\u003e\n\u003cstrong\u003eAFG-VZ-N203-01\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (24 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"114\"\u003e\n\u003cstrong\u003eAFG-VZ-N203-02\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (96 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnd Prep Mix 4\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e360 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4 X 360 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003cbr\u003eStore at -30 ~ -15℃ and transport at ≤0℃.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385849217355,"sku":"AFG-M-0173","price":216.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":46666160898379,"sku":"AFG-M-0174","price":816.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_0ce0b970-03e9-4285-bcad-540ae6e08959.png?v=1741089527"},{"product_id":"affings-universal-adapter-ligation-module-for-illumina-v2","title":"AffiNGS® Universal Adapter Ligation Module for Illumina V2","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® Universal Adapter Ligation Module for Illumina V2\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiNGS® Universal Adapter Ligation Module for Illumina V2\u003c\/strong\u003e is a directional  optimized Ligation module for library construction of Illumina high-throughput sequencing platform . This kit can be used to efficiently ligate the Adapter containing dT tail to the end of the repaired product containing phosphate group at the 5' end and dA tail at the 3' end. Compared with the traditional method, Universal Adapter Ligation Module for Illumina V2 uses one-tube reaction. The fragmented DNA is treated by Universal End preparation Module for Illumina V2 (AffiNGS#\u003cspan class=\"variant-sku\" data-mce-fragment=\"1\"\u003eAFG-VZ-\u003c\/span\u003eN203), which can be directly ligated to the Adapter without additional product purification steps. Therefore, the minimum requirement of initial DNA template is significantly reduced and the experiment time is shortened. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"margin-left: auto; margin-right: auto;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\" style=\"text-align: center;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"15%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-VZ-N204-01 (24 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd width=\"15%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-VZ-N204-02 (96 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRapid Ligation Buffer 2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e600 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4 X 600 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRapid DNA Ligase\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e120 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e480 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eStore at -30 ~ -15℃ and transport at ≤0℃.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385849119051,"sku":"AFG-VZ-N204-01","price":360.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":51385849151819,"sku":"AFG-VZ-N204-02","price":1345.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_a200a314-80c7-477d-87fc-fe9616e36f5c.png?v=1741089526"},{"product_id":"affings-dna-damage-repair-kit","title":"AffiNGS® DNA Damage Repair Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® DNA Damage Repair Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eOptimized enzyme mix for repairing damaged DNA, especially from FFPE (formalin-fixed paraffin-embedded) samples.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003eKey Benefits:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh repair efficiency for FFPE-derived DNA.\u003c\/li\u003e\n\u003cli\u003eReaction buffer compatible with end repair\/adenylation workflows.\u003c\/li\u003e\n\u003cli\u003eNo further purification needed after using thermosensitive proteinase K.\u003c\/li\u003e\n\u003cli\u003eDirect integration into library construction workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/span\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"10%\" style=\"text-align: left;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"15%\"\u003e\u003cstrong\u003e AFG-BCL-000001\u003cbr\u003e(24 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"15%\"\u003e\u003cstrong\u003e AFG-BCL-000002\u003cbr\u003e(96 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDamaged DNA Repair Enzymes\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e72 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e288 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eStorage: −20°C to −10°C.\u003c\/li\u003e\n\u003cli\u003eShipping: Use dry ice or combination of dry ice and ice packs, maintain between −40°C to −20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e\u003cstrong\u003eProtocol:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eImportant Notes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli style=\"font-weight: bold;\"\u003e\n\u003cstrong\u003eUse only with severely damaged DNA such as FFPE samples.\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: bold;\"\u003e\u003cstrong\u003e\u003cmeta charset=\"utf-8\"\u003eNot compatible with Cell-free DNA (cfDNA) – may cause random fragmentation.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: bold;\"\u003e\u003cstrong\u003e\u003cmeta charset=\"utf-8\"\u003eNot compatible with Tissue or high-quality genomic DNA – unnecessary and may not be effective.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 style=\"font-weight: bold;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e1. Recommended DNA Input Amount:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eSample Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eSuggested Input DNA (mass)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003eFFPE DNA\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e100–500 ng\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eRecommended range\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e200–300 ng\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eCommon working range\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003eUp to 1 µg\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eHigher input gives better libraries (if available)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u0026lt;100 ng\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003ePossible for rare samples; expect lower yield\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e2. Reaction Setup (on ice):\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003eCompatible with ultrasonic-based fragmentation protocols.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 19.5938px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 35%; height: 19.5938px;\"\u003e\u003cstrong\u003e Reagent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 30%; height: 19.5938px;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 35%; height: 19.5938px;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 35%;\"\u003eFragmented FFPE DNA\u003c\/td\u003e\n\u003ctd style=\"width: 30%;\"\u003eX μL\u003c\/td\u003e\n\u003ctd style=\"width: 35%;\"\u003eAdjust to desired input amount (see above)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 35%;\"\u003eEnd Repair Buffer (from your kit)\u003c\/td\u003e\n\u003ctd style=\"width: 30%;\"\u003eY μL\u003c\/td\u003e\n\u003ctd style=\"width: 35%;\"\u003eAs per your library kit's recommendation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 35%;\"\u003eEnd Repair Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"width: 30%;\"\u003eZ μL\u003c\/td\u003e\n\u003ctd style=\"width: 35%;\"\u003eAs per your library kit's recommendation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 35%;\"\u003eDamaged DNA Repair Enzymes\u003c\/td\u003e\n\u003ctd style=\"width: 30%;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 35%;\"\u003eEnhances repair of damaged FFPE DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 35%;\"\u003eNuclease-free Water\u003c\/td\u003e\n\u003ctd style=\"width: 30%;\"\u003eUp to final volume (e.g., 60 μL)\u003c\/td\u003e\n\u003ctd style=\"width: 35%;\"\u003eAdjust to total reaction volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e3. Mixing \u0026amp; Incubation:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMix thoroughly by pipetting.\u003c\/li\u003e\n\u003cli\u003eBriefly centrifuge.\u003c\/li\u003e\n\u003cli\u003eIncubate using a PCR thermocycler (lid temperature: 105°C):\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"width: 100%;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003e\u003cstrong\u003e Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e\u003cstrong\u003eDuration\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003e30°C\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e30 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003e65°C\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e30 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003e4°C\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eHold\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003e\u003cstrong\u003e4. Next Steps:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eProceed directly to:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdapter ligation\u003c\/li\u003e\n\u003cli\u003ePurification\u003c\/li\u003e\n\u003cli\u003eLibrary amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFollow your library prep kit's instructions for all subsequent steps.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385848987979,"sku":"AFG-BCL-000001","price":450.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":51385849020747,"sku":"AFG-BCL-000002","price":780.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_e76c51f9-fe6f-4bac-8e17-2ba136aa1927.png?v=1741089525"},{"product_id":"affi-universal-plus-fragmentation-end-preparation-da-tailing-module-for-illumina-v2","title":"AffiNGS® Universal Plus Fragmentation,End Preparation \u0026 dA-Tailing Module for Illumina V2","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® Universal Plus Fragmentation,End Preparation \u0026amp; dA-Tailing Module for Illumina V2\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiNGS® Universal Plus Fragmentation,End Preparation \u0026amp; dA-Tailing Module for Illumina V2\u003c\/strong\u003e is developed specifically for Illumina high-throughput sequencing platform, support fragmentation, end-repair and dA-tailing ina single reaction step.The module offerts improved fragmentation performance, which significantly reduces the proportion of Artificial Invert Chimera Reads in the FFPE sample DNA library and improves the reliability of SNV detection. The module has been optimized to convert 100 pg - 1 µg of DNA to fragmented, end-repaired DNA having 5' phosphorylated, 3' dA-tailed ends and qualified with automation methods, greatly reducing the total operation time. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponents:\u003c\/strong\u003e \u003c\/p\u003e\n\u003ctable width=\"456\" style=\"height: 97.9689px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd width=\"196\" style=\"text-align: left; height: 39.1875px;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"130\" style=\"text-align: center; height: 39.1875px;\"\u003e\n\u003cstrong\u003eAFG-VZ-N219-01\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (24 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd width=\"130\" style=\"text-align: center; height: 39.1875px;\"\u003e\n\u003cstrong\u003eAFG-VZ-N219-02\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (96 rxns)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003eFEA Buffer V2\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e120 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e480 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003eFEA Enzyme Mix V2\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e240 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e960 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\"\u003eNeutralization Buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e120 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 19.5938px;\"\u003e480 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30 ~-15°C and transport at ≤0° C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385848856907,"sku":"AFG-M-0167","price":264.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":51385848889675,"sku":"AFG-M-0168","price":960.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_0085ace9-852c-4388-b466-91df7390aa7a.png?v=1741089524"},{"product_id":"affi-hifi-universal-amplification-mix-for-illumina","title":"AffiNGS® HiFi Universal Amplification Mix for Illumina","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiNGS® HiFi Universal Amplification Mix for Illumina\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiNGS® HiFi Universal Amplification Mix for Illumina\u003c\/strong\u003e is an upgraded version for HiFi Amplification Mix, which is applicable to high-throughput sequencing library PCR amplification. After modification on HiFi DNA Polymerase, the template compatibility and sensitivity of this enzyme have been greatly improved; with an optimized buffer system, HiFi Universal Amplification Mix for Illumina can amplify the library with high uniformity, increasing the sequencing quality. It contains special protective regents, which enable long-term storage and can maintain a stable activity after repeated freezing and thawing. HiFi Universal Amplification Mix for Illumina has undergone rigorous quality control and functional testing to ensure optimal stability and repeatability.\u003c\/p\u003e\n\u003ch3 class=\"fl-heading\"\u003e\n\u003cspan class=\"fl-heading-text\"\u003eComponents\u003c\/span\u003e:\u003c\/h3\u003e\n\u003ctable width=\"515\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"261\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"128\" style=\"text-align: center;\"\u003e\u003cstrong\u003e AFG-M-0402 (24 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"126\" style=\"text-align: center;\"\u003e\u003cstrong\u003e AFG-M-0403 (96 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"261\"\u003eHiFi Universal Amplification Mix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e600 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4 X 600 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"261\"\u003ePCR Primer Mix for Illumina\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e120 µl\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e480 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-628369e85d24b\" data-node=\"628369e85d24b\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv class=\"fl-col-group fl-node-628369e85d603\" data-node=\"628369e85d603\"\u003e\n\u003cdiv class=\"fl-col fl-node-628369e85d64b\" data-node=\"628369e85d64b\" style=\"width: 100%;\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-module fl-module-heading fl-node-628369e85d68f pro_tit\" data-node=\"628369e85d68f\" data-animation-delay=\"0.0\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fl-module fl-module-rich-text fl-node-628369e85d6d3\" data-node=\"628369e85d6d3\" data-animation-delay=\"0.0\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eThe Kit should be stored at -30 ~ -15℃ and transported at ≤0℃.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385848561995,"sku":"AFG-M-0402","price":77.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":51385848594763,"sku":"AFG-M-0403","price":268.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_66ef0adc-7c28-43ec-8fd6-94c390199fac.png?v=1741089523"},{"product_id":"affi-ampseq-multi-pcr-module-v3","title":"AffiNGS® AmpSeq Multi-PCR Module V3","description":"\u003ch2\u003e\n\u003cspan style=\"color: #429593;\"\u003eAffiNGS® AmpSeq Multi-PCR Module V3\u003c\/span\u003e \u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiNGS® AmpSeq Multi-PCR Module V3\u003c\/strong\u003e is an amplification reagent based un super-multiplex PCR technique, used to construct amplicon librarie. It is compatible with gDNA, FFPE DNA, cfDNA, and other sample types with Input DNA of 1 - 100 ng.This optimized reagent contains enzymes dNTPs and reaction buffer required for multiplex PCR.This reagent has great amplification performance for different input amounts and customized panel, which ensure the high coverage and high uniformity of the amplicon library. It is suitable for the library preparation for amplicon sequencing. helping researchers and technical personnel to perform super-multiplex PCR quickly and easily with. The reagent has undergone rigorous quality control and functional validation to ensure the optimal stability and repeatability of library construction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":51385848430923,"sku":"AFG-M-0405","price":120.0,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":51385848463691,"sku":"AFG-M-0406","price":412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_66b4f07c-5ee2-468f-976b-4559775d4e2a.png?v=1741089522"}],"url":"https:\/\/affigen.com\/nl-nl\/collections\/dna-library-preparation-module.oembed","provider":"AffiGEN","version":"1.0","type":"link"}