{"title":"AffiASSAY®","description":"\u003cstyle\u003e\u003c!--\n  \/* Center the search bar *\/\n  .centered-search {\n    display: flex;\n    justify-content: center;\n    align-items: center;\n    height: 100px; \/* You can adjust the height as needed *\/\n  }\n\n  \/* Style the search input and button *\/\n  .search-input {\n    width: 70%; \/* Adjust the width as needed *\/\n    padding: 10px;\n    border: 4px solid #992763;\n    border-radius: 25px;\n    margin-right: 5px;\n  }\n\n  .search-button {\n    background-color: #992763; \/* Change to your desired button color *\/\n    color: white;\n    padding: 10px 20px;\n    border: 4px solid #992763;\n    border-radius: 25px;\n    cursor: pointer;\n  }\n--\u003e\u003c\/style\u003e\n\u003cdiv class=\"centered-search\"\u003e\n\u003cinput placeholder=\"Search in our Assay Kits Database ...\" name=\"q\" class=\"search-input\" type=\"text\" onkeydown=\"handleEnterKey(event)\"\u003e \u003cbutton class=\"search-button\" type=\"button\" onclick=\"showIframe()\"\u003eSearch\u003c\/button\u003e\n\u003c\/div\u003e\n\u003cscript\u003e\n  function handleEnterKey(event) {\n    if (event.key === \"Enter\") {\n      showIframe();\n      event.preventDefault(); \/\/ Prevent the default form submission behavior\n    }\n  }\n\n  function showIframe() {\n    var searchQuery = encodeURIComponent(document.querySelector('.search-input').value);\n    var searchUrl = 'https:\/\/www.affiassay.com\/website\/search?search=' + searchQuery; \/\/ Replace with the target website's search URL\n    \n    window.open(searchUrl, '_blank'); \/\/ Open the search results in a new window\/tab\n  }\n\u003c\/script\u003e","products":[{"product_id":"affiassay-dual-luciferase-reporter-assay-kit","title":"AffiASSAY® Dual Luciferase Reporter Assay Kit","description":"\u003ch2\u003eAffiASSAY® Dual Luciferase Reporter Assay Kit\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eAffiASSAY® Dual Luciferase Reporter Assay Ki\u003c\/strong\u003et is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter gene detection, Firefly luciferase is detected with Luciferin as a substrate, and Renilla Iuciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity. Renilla Iuciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and Renilla luciferase catalyzes the emission of coelenterazine at 465 nm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents\u003c\/td\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5 × Cell Lysis Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction Buffer II (Luciferase)\u003c\/td\u003e\n\u003ctd\u003e\u003cspan data-mce-fragment=\"1\"\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLuciferase Substrate (Lyophilized)\u003c\/td\u003e\n\u003ctd\u003e1 Vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop \u0026amp; Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRenilla Substrate\u003c\/td\u003e\n\u003ctd\u003e200 uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30 ~ -15℃ and transport at ≤0℃. Dissolved and dispensed Reagent: long-term storage at -85 ~ -65℃, or short-term storage at -30 ~ -15℃ for no more than one month; Stop \u0026amp; Reaction Buffer: long-term storage at 2 ~ 8℃.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelf-prepared Materials:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt is applicable for the detection of cell gene regulation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWhen used for the first time, the Reaction Buffer II (Luciferase) should be poured into a brown dark bottle containing lyophilized Luciferase substrate.\u003c\/li\u003e\n\u003cli\u003eMix thoroughly and dispense according to the needs of use and protect from light at -70℃. Mix 5 × Cell Lysis Buffer and ddH2O in a ratio of 1:4 before use, and place on ice for use.\u003c\/li\u003e\n\u003cli\u003eDissolve Renilla Substrate in absolute ethanol. Please centrifuge briefly for the first use and carefully measure the volume of the solution in the tube. If the liquid volume is found to be significantly reduced, please add absolute ethanol to make up the volume before saving.\u003c\/li\u003e\n\u003cli\u003ePlace Renilla Substrate on ice during use. Calculate the usage amount, mix an appropriate amount of Stop \u0026amp; Reaction Buffer and Renilla Substrate at a ratio of 50:1. Store at room temperature and protect from light for later use.\u003c\/li\u003e\n\u003cli\u003eThe enzymatic reaction is sensitive to temperature, so the cell lysis solution and the detection substrate solution should be equilibrated to room temperature before the sample is applied for testing.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Reactions (100 μl\/Reaction)","offer_id":46663604765003,"sku":"AFG-M-0229","price":181.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiASSAY-Camera2.png?v=1741089581"},{"product_id":"affiassay-bca-protein-quantification-kit","title":"AffiASSAY® BCA Protein Quantification Kit","description":"\u003ch2\u003e\u003cstrong\u003eAffiASSAY® BCA Protein Quantification Kit\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cmeta charset=\"utf-8\"\u003eAffiASSAY® BCA Protein Quantification Kit\u003c\/strong\u003e is one of the most sensitive methods for measuring protein concentration. The protein reduces Cu2+ to Cu+ under alkaline conditions. Then, Cu+ interacts with the unique BCA Reagent A (containing BCA) to preform a sensitive color reaction, forming coordination complexs. The water-soluble complex has strong absorbance at A562 nm. Its absorbance and protein concentration have a good linear relationship in a wide range, and the protein concentration can be calculated according to the absorbance value. Therefore, the concentration of the protein to be tested can be calculated by measuring its absorption value at A562 nm using a microplate reader and comparing it with the standard curve. Common concentrations of detergents (SDS, Triton X-100, Tween-20, etc.) do not affect the detection results, but they are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. In the experiment, if sample dilution or lysis solution with high background values, it is recommended to use Bradford protein assay.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBSA Standard (1 mg\/ml)\u003c\/td\u003e\n\u003ctd\u003e2 × 1 mL\u003c\/td\u003e\n\u003ctd\u003e4\u003cspan data-mce-fragment=\"1\"\u003e 1 mL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBCA Reagent A\u003c\/td\u003e\n\u003ctd\u003e 50 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBCA Reagent B\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e2 X 1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Condition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eBCA Reagent A\/B: Store at 15 ~ 25℃ and transport at room temperature. BSA Standard: Store at -30 ~ -15℃ and transport at ≤0℃.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt is applicable for protein samples extracted by common lysis solution. The performance is not affected at common concentrations of most ionic and non-ionic detergents.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"250 Reactions","offer_id":46663605354827,"sku":"AFG-M-0247","price":90.0,"currency_code":"USD","in_stock":true},{"title":"500 Reactions","offer_id":51385854820683,"sku":"AFG-M-0248","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiASSAY-Camera2_317a6344-82c8-4350-8012-2deb0245e7e7.png?v=1741089578"},{"product_id":"affiassay-total-carbohydrate-assay-kit","title":"AffiASSAY® Total Carbohydrate Assay Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cspan style=\"color: #429593;\"\u003eAffiASSAY® Total Carbohydrate Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAffiASSAY® Total Carbohydrate Assay Kit is designed for precision and ease of use, this kit is your key to accurate carbohydrate quantification in various sample types.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKey Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAccurate Carbohydrate Quantification:\u003c\/strong\u003e Achieve precise measurements of total carbohydrates in your samples, from simple sugars to complex polysaccharides.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eVersatile Compatibility:\u003c\/strong\u003e Suitable for a wide range of sample matrices, including biological fluids, food products, and plant extracts.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSensitivity and Specificity:\u003c\/strong\u003e Detect a broad range of carbohydrate structures with high sensitivity and specificity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eRobust Assay Performance:\u003c\/strong\u003e Reliable and reproducible results for consistent data in your research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eEasy-to-Follow Protocol:\u003c\/strong\u003e Streamlined protocol with straightforward steps for quick and efficient analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eNutrition and Food Science\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eGlycobiology Research\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eClinical Diagnostics\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eEnvironmental Studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eBenefits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eComprehensive carbohydrate analysis\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eVersatile compatibility with various sample types\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh sensitivity and specificity\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eReliable and reproducible results\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSimplified workflow for efficient analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eEach Kit Contains:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eReagents for up to [number] assays (based on [sample volume])\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eDetailed user manual with step-by-step instructions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eUnlock the Carbohydrate Mysteries in Your Samples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eEnhance your research with the AffiASSAY® Total Carbohydrate Assay Kit, your trusted companion for carbohydrate analysis. Whether you're studying food products, biological samples, or environmental materials, our kit provides the accuracy and simplicity you need to uncover valuable insights.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":46868536623435,"sku":"AFG-ASY-001","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affiassay-affigen.png?v=1693569738"},{"product_id":"affiassay-rt-lampdyeassaykit-udg-afg-ysn-284","title":"AffiASSAY® RT-LAMP Dye Assay Kit (UDG+)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRT-LAMP Dye Assay Kit (UDG+) contains Bst Plus DNA Polymerase and heat-resistant III Reverse Transcriptase, MRI, and UDGas applyed to LAMP reaction with RNA as template. This product contains UDG enzyme, which can effectively prevent the risk of aerosol pollution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100 Tests","offer_id":47092217381195,"sku":"AFG-YSN-284","price":313.8,"currency_code":"USD","in_stock":true},{"title":"1000 Tests","offer_id":47092217413963,"sku":"AFG-YSN-285","price":2061.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_e2eae718-d4fc-4988-84ed-2e4f312f4913.png?v=1699871222"},{"product_id":"affiassay-bca-protein-quantification-kit-afg-ysn-302","title":"AffiASSAY® BCA Protein Quantification Kit","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eBCA (Bicinchoninic acid) method is a widely used method for protein concentration determination at present. Based on the biuret reaction, that is, in an alkaline environment, the protein reduces Cu2+ to Cu+, producing a purple blue complex, which has a high absorbance value at 562 nm. The amount of the reaction product is proportional to the protein concentration. BCA protein concentration determination method is simple, sensitive, fast and stable. The protein standard provided in the kit provides convenience for users to make standard curves.\n The BCA protein concentration determination kit can be used for colorimetric assay and microplate assay. The former requires a large amount (100 μL) of protein sample, The ratio of protein sample to BCA working solution is 1: 20 (v\/v) , which reduces the impact of interfering substances. The latter is simple and convenient to operate, requiring only a small amount (10-25 μL) of protein sample. However, because the ratio of protein sample to BCA working solution is 1: 8 (v\/v) , the tolerance concentration of interfering substances is limited to some extent and the minimum detection level is reduced. Our company provides three sizes of BCA protein concentration detection kits, the colorimetric method can be used for 50 times, 250 times and 500 times respectively. The enzyme labeling method can be used for 500 times, 2500 times and 5000 times respectively.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 x 60T (12 mL)","offer_id":47092219085131,"sku":"AFG-YSN-302","price":213.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_6bdbf683-b304-4e38-9fa6-4b8b6991fb5c.png?v=1699871243"},{"product_id":"affiassay-bca-protein-quantification-kit-bca-afg-ysn-303","title":"AffiASSAY® BCA Protein Quantification Kit BCA","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eBCA (Bicinchoninic acid) method is a widely used method for protein concentration determination at present. Based on the biuret reaction, that is, in an alkaline environment, the protein reduces Cu2+ to Cu+, producing a purple blue complex, which has a high absorbance value at 562 nm. The amount of the reaction product is proportional to the protein concentration. BCA protein concentration determination method is simple, sensitive, fast and stable. The protein standard provided in the kit provides convenience for users to make standard curves.\n The BCA protein concentration determination kit can be used for colorimetric assay and microplate assay. The former requires a large amount (100 μL) of protein sample, The ratio of protein sample to BCA working solution is 1: 20 (v\/v) , which reduces the impact of interfering substances. The latter is simple and convenient to operate, requiring only a small amount (10-25 μL) of protein sample. However, because the ratio of protein sample to BCA working solution is 1: 8 (v\/v) , the tolerance concentration of interfering substances is limited to some extent and the minimum detection level is reduced. Our company provides three sizes of BCA protein concentration detection kits, the colorimetric method can be used for 50 times, 250 times and 500 times respectively. The enzyme labeling method can be used for 500 times, 2500 times and 5000 times respectively.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5 x 500 Tests (100 mL)","offer_id":47092219380043,"sku":"AFG-YSN-303","price":591.0,"currency_code":"USD","in_stock":true},{"title":"2500 Tests(500 mL)","offer_id":47092219412811,"sku":"AFG-YSN-304","price":481.8,"currency_code":"USD","in_stock":true},{"title":"5000 Tests(1000)","offer_id":47092219445579,"sku":"AFG-YSN-305","price":743.88,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_01e53c68-3512-4e29-9a62-ed2b0eb31818.png?v=1699871245"},{"product_id":"affiassay-bradford-protein-quantification-kit-afg-ysn-306","title":"AffiASSAY® Bradford Protein Quantification Kit","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eBradford method is one of the most sensitive methods used to determine protein concentration. It is based on the Bradford dye (Coomassie brilliant blue G250) and protein combination, the maximum absorption peak of dye from A456 to A595, and the determination of the absorption value and protein concentration is proportional to the principle of design. The method can calculate the protein concentration by the light absorption value and realize the rapidity and simplicity of protein concentration determination. High sensitivity, about four times higher than Lowry method, the lowest protein detection can reach 1 μg. The determination is fast and simple, requiring only one reagent, and is not affected by the chemical reagents in most samples.\n Our company provides two sizes of Bradford protein concentration detection kits, the colorimetric method can be used 125 times, and 625 times respectively. The enzyme labeling method can be used 500 times and 2500 times respectively.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5 x 500 Tests (125 mL)","offer_id":47092219478347,"sku":"AFG-YSN-306","price":612.0,"currency_code":"USD","in_stock":true},{"title":"2500 Tests(625 mL)","offer_id":47092219511115,"sku":"AFG-YSN-307","price":498.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_39e83a87-9762-4a76-a53f-5cbd1271ac8e.png?v=1699871248"},{"product_id":"affiassay-enhanced-ecl-chemiluminescent-substrate-kit-afg-ysn-375","title":"AffiASSAY® Enhanced ECL Chemiluminescent Substrate Kit","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eEnhanced ECL Chemiluminescent Substrate Kit is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP) . The principle of Enhanced ECL Chemiluminescent Substrate Kit is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.\n This kit has a unique luminescent substrate system, reducing the exposure background while introducing a new oxidant greatly improves the stability of the kit, allowing it to be stable at room temperature for up to one year. In addition to X-ray, fluorescent CCD scans can also be directly used mainly for WB detection and chemiluminescence immune detection systems.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5 x 100 mL","offer_id":47092228096331,"sku":"AFG-YSN-375","price":276.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47092228129099,"sku":"AFG-YSN-376","price":200.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_6a1908ac-685c-467e-9b31-c5a266ce570c.png?v=1699871319"},{"product_id":"affiassay-cell-counting-kit-cck-8-afg-ysn-415","title":"AffiASSAY® Cell Counting Kit 8 (WST-8 \/ CCK-8)","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan data-mce-fragment=\"1\" style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cell Counting Kit 8 (WST-8 \/ CCK-8)\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Cell Counting Kit 8 (WST-8 \/ CCK-8) is a reliable and easy-to-use assay designed for the rapid and accurate quantification of cell viability and proliferation. This kit utilizes a colorimetric method based on the reduction of a water-soluble tetrazolium salt (WST-8) by cellular dehydrogenases in viable cells to produce an orange-colored formazan dye. The intensity of the dye is directly proportional to the number of living cells in the sample, providing a sensitive measure of cell viability and proliferation.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e Rapid and sensitive:\u003c\/strong\u003e Provides accurate results within a short incubation period.\u003cbr data-mce-fragment=\"1\"\u003eWide applicability: Compatible with a variety of cell types, including adherent and suspension cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh throughput:\u003c\/strong\u003e Suitable for high-throughput screening applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Can be used for cell proliferation assays, cytotoxicity assays, and drug screening assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStable signal:\u003c\/strong\u003e Offers stable and reproducible results over a wide range of cell densities and experimental conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe AffiASSAY® CCK-8 kit utilizes the water-soluble tetrazolium salt WST-8, which is reduced by cellular dehydrogenases in viable cells to form a water-soluble formazan dye. The intensity of the orange-colored formazan dye produced is directly proportional to the number of living cells in the sample. The absorbance of the formazan dye can be measured spectrophotometrically at a wavelength of 450 nm using a microplate reader.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e Cell viability assays:\u003c\/strong\u003e Determine the number of viable cells in culture.\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell proliferation assays:\u003c\/strong\u003e Assess the rate of cell proliferation and growth.\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCytotoxicity assays:\u003c\/strong\u003e Evaluate the cytotoxic effects of compounds or treatments on cells.\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug screening assays:\u003c\/strong\u003e Screen potential drug candidates for their effects on cell viability and prol\u003cspan data-mce-fragment=\"1\"\u003eiferation.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCCK-8 reagent: WST-8 tetrazolium salt solution\u003c\/li\u003e\n\u003cli\u003eUser manual: Detailed instructions for assay setup and data analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eStorage and Stability:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eThe components of the AffiASSAY® CCK-8 kit should be stored at the recommended temperature (-20°C) and protected from light. When stored properly, the kit is stable for up to 24 months from the date of manufacture.\u003c\/li\u003e\n\u003cli\u003eThe kits is stable for 12 months at +4°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003ePrecautions:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFor research use only. Not for diagnostic or therapeutic purposes.\u003c\/li\u003e\n\u003cli\u003eHandle all reagents with care and follow the instructions provided in the user manual.\u003c\/li\u003e\n\u003cli\u003eAvoid exposure of reagents to light during storage and assay procedures.\u003c\/li\u003e\n\u003cli\u003eUse appropriate safety precautions when working with cell cultures and laboratory chemicals.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eReferences:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDoi, K., et al. (1995). A novel water-soluble tetrazolium salt for colorimetric cell viability assay. Analytical Biochemistry, 227(2), 328-335.\u003c\/li\u003e\n\u003cli\u003eOhno, R., et al. (1993). A novel water-soluble tetrazolium salt for non-enzymatic cell viability assay. Oncology Reports, 31(5), 2379-2383.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cell Counting Kit 8 (WST-8 \/ CCK-8) Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Cell Counting Kit (CCK-8)\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003eCell culture medium\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003eCells of interest\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003ePrepare Cell Culture:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eCulture cells of interest according to standard protocols in appropriate culture vessels until they reach the desired confluence or growth phase.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003ePrepare Working Solution:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThaw the CCK-8 reagent and allow it to equilibrate to room temperature.\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003ePrepare the CCK-8 working solution by diluting the CCK-8 reagent with fresh cell culture medium at a ratio of 1:10 (e.g., 10 μL of CCK-8 reagent + 90 μL of medium per well). Prepare enough working solution for all wells to be assayed.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSeed Cells in Microplate:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAspirate the culture medium from the cells and wash once with phosphate-buffered saline (PBS) if necessary.\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eAdd the appropriate volume of fresh cell culture medium containing cells to each well of a 96-well microplate. Adjust the cell density according to the experimental requirements.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubate Cells with CCK-8 Reagent:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate containing cells with the prepared CCK-8 working solution. Ensure each well is evenly covered with the solution.\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at standard cell culture conditions (e.g., 37°C, 5% CO2) for a specified period of time (typically 1-4 hours). The optimal incubation time may vary depending on the cell type and experimental conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasure Absorbance:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, gently mix the contents of each well by pipetting up and down or using a plate shaker.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance of each well at 450 nm using a microplate reader. Ensure to use a reference wavelength if necessary according to the manufacturer's instructions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eRecord the absorbance values for each well.\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCalculate the cell viability or proliferation using the formula provided by the manufacturer or based on the specific experimental design.\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eAnalyze the data and interpret the results accordingly.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eQuality Control:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePerform appropriate controls, including background controls (no cells) and positive controls (e.g., untreated cells or cells treated with known compounds).\u003c\/li\u003e\n\u003cli\u003eEnsure all reagents are handled and stored properly according to the manufacturer's instructions to maintain assay reliability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eTroubleshooting:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIf unexpected results are obtained, troubleshoot the assay by checking experimental conditions, reagent handling, and protocol adherence.\u003c\/li\u003e\n\u003cli\u003eConsult the user manual or contact technical support for further assistance if needed..\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eDispose of Reagents:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDispose of all reagents and materials according to local regulations for biohazardous waste or chemical waste disposal.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47092235600203,"sku":"AFG-YSN-415","price":80.0,"currency_code":"USD","in_stock":true},{"title":"2 x 1 mL","offer_id":47092235632971,"sku":"AFG-YSN-416","price":120.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":47092235665739,"sku":"AFG-YSN-417","price":135.0,"currency_code":"USD","in_stock":true},{"title":"6 x 5 mL","offer_id":47092235698507,"sku":"AFG-YSN-418","price":320.0,"currency_code":"USD","in_stock":true},{"title":"24 x 5 mL","offer_id":47092235731275,"sku":"AFG-YSN-419","price":860.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiASSAY-Camera2_1500x_dd14db46-66fc-46ef-9ab5-27b81232487a.png?v=1699871402"},{"product_id":"affiassay-acetyl-coa-fluorometric-assay-kit","title":"AffiASSAY® Acetyl-CoA Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Acetyl-CoA Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Acetyl-CoA Fluorometric Assay Kit is designed to provide precise and reliable measurements of Acetyl-CoA levels in a variety of biological samples, this assay kit is a powerful tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of Acetyl-CoA, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Acetyl-CoA Fluorometric Assay Kit can be used with a broad range of sample types, including cells, tissues, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cellular metabolism, investigating the effects of drug compounds, or exploring the intricacies of enzymatic reactions, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a fluorometric method based on the enzymatic conversion of Acetyl-CoA into a fluorescent product. The increase in fluorescence intensity is directly proportional to the Acetyl-CoA concentration in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Research:\u003c\/strong\u003e Study the role of Acetyl-CoA in cellular energy production and lipid metabolism.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Development:\u003c\/strong\u003e Assess the impact of pharmaceutical compounds on Acetyl-CoA levels, aiding in drug discovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Kinetics:\u003c\/strong\u003e Investigate the kinetics of Acetyl-CoA-utilizing enzymes with accuracy and ease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Explore the connections between Acetyl-CoA and cellular signaling pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eAcetyl-CoA Standard\u003c\/li\u003e\n\u003cli\u003eEnzyme Mix\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Acetyl-CoA Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Acetyl-CoA Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing Acetyl-CoA\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring fluorescence (excitation: ~535-575 nm, emission: ~585-625 nm)\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing Acetyl-CoA. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Acetyl-CoA Standard provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Acetyl-CoA standard to generate a standard curve. Typical concentrations may range from 0 to 100 µM.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a control sample without Acetyl-CoA to serve as a background control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample Dilution:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDilute your biological samples as needed to ensure that they fall within the linear range of the standard curve. The specific dilution factor will depend on the expected Acetyl-CoA concentration in your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer and Enzyme Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your diluted samples and Acetyl-CoA standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic conversion of Acetyl-CoA to a fluorescent product.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader with appropriate fluorescence settings, measure the fluorescence intensity of each well. Excitation should be around 535-575 nm, and emission around 585-625 nm.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the fluorescence values obtained from the Acetyl-CoA standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the Acetyl-CoA concentrations in your samples based on their respective fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in Acetyl-CoA levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Acetyl-CoA Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47292867936587,"sku":"AFG-BV-0016","price":622.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/IMG_1195.jpg?v=1705229752"},{"product_id":"affiassay-atp-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® ATP Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003cstyle\u003e\n  .afg-atp-page {\n    font-family: Arial, Helvetica, sans-serif;\n    color: #2f3745;\n    line-height: 1.55;\n    background: #ffffff;\n    width: 100%;\n    max-width: none;\n    margin: 0;\n    padding: 44px 52px 52px;\n    border-top: 5px solid #1aa6a8;\n  }\n\n  .afg-atp-page * {\n    box-sizing: border-box;\n  }\n\n  .afg-atp-hero {\n    width: 100%;\n    padding: 0 0 34px;\n    margin-bottom: 36px;\n    border-bottom: 1px solid #d7eeee;\n  }\n\n  .afg-atp-eyebrow {\n    color: #b85b91;\n    font-weight: 700;\n    text-transform: uppercase;\n    letter-spacing: 0.08em;\n    font-size: 13px;\n    margin-bottom: 8px;\n  }\n\n  .afg-atp-title {\n    color: #178f92;\n    font-size: 36px;\n    line-height: 1.16;\n    margin: 0 0 16px;\n    font-weight: 800;\n  }\n\n  .afg-atp-subtitle {\n    font-size: 17px;\n    color: #4c5664;\n    max-width: 100%;\n    margin: 0;\n  }\n\n  .afg-atp-badges {\n    display: flex;\n    flex-wrap: wrap;\n    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  background: #fff8fb;\n    padding: 20px 24px;\n    margin: 24px 0;\n  }\n\n  .afg-atp-note {\n    border-left: 5px solid #1aa6a8;\n    background: #f5fcfc;\n    padding: 20px 24px;\n    margin: 24px 0;\n  }\n\n  .afg-atp-image {\n    margin: 28px 0;\n    text-align: center;\n    width: 100%;\n  }\n\n  .afg-atp-image img {\n    width: 100%;\n    max-width: 100%;\n    height: auto;\n    display: block;\n    margin: 0 auto;\n    border: 1px solid #d7eeee;\n  }\n\n  .afg-atp-image-caption {\n    font-size: 14px;\n    color: #6b7280;\n    margin-top: 10px;\n  }\n\n  .afg-atp-table-wrap {\n    overflow-x: auto;\n    margin: 20px 0;\n    width: 100%;\n  }\n\n  .afg-atp-table {\n    width: 100%;\n    border-collapse: collapse;\n    font-size: 14px;\n    min-width: 640px;\n  }\n\n  .afg-atp-table th {\n    background: #178f92;\n    color: #ffffff;\n    text-align: left;\n    padding: 13px 16px;\n    border: 1px solid #178f92;\n    font-weight: 700;\n  }\n\n  .afg-atp-table td {\n    border: 1px solid #d7eeee;\n    padding: 13px 16px;\n    vertical-align: top;\n  }\n\n  .afg-atp-table tr:nth-child(even) td {\n    background: #f8fcfc;\n  }\n\n  .afg-atp-steps {\n    counter-reset: afgstep;\n    margin: 20px 0 0;\n    padding: 0;\n  }\n\n  .afg-atp-steps li {\n    counter-increment: afgstep;\n    list-style: none;\n    border-left: 4px solid #1aa6a8;\n    padding: 12px 0 12px 18px;\n    margin-bottom: 9px;\n    background: #fbfefe;\n  }\n\n  .afg-atp-steps li::before {\n    content: counter(afgstep) \". \";\n    color: #b85b91;\n    font-weight: 800;\n  }\n\n  .afg-atp-formula {\n    background: #f8fcfc;\n    border: 1px solid #d7eeee;\n    padding: 16px 18px;\n    font-family: Arial, Helvetica, sans-serif;\n    font-weight: 700;\n    color: #2f3745;\n    margin: 14px 0;\n  }\n\n  .afg-atp-list {\n    margin: 0;\n    padding-left: 20px;\n  }\n\n  .afg-atp-list li {\n    margin-bottom: 8px;\n  }\n\n  .afg-atp-cta {\n    width: 100%;\n    margin-top: 46px;\n    padding: 34px 36px;\n    background: #178f92;\n    color: #ffffff;\n  }\n\n  .afg-atp-cta h2 {\n    color: #ffffff;\n    border-bottom: 1px solid rgba(255,255,255,0.35);\n    margin-top: 0;\n  }\n\n  .afg-atp-cta p {\n    margin-bottom: 0;\n  }\n\n  @media (max-width: 760px) {\n    .afg-atp-page {\n      padding: 28px 20px 36px;\n    }\n\n    .afg-atp-title {\n      font-size: 29px;\n    }\n\n    .afg-atp-grid {\n      grid-template-columns: 1fr;\n    }\n\n    .afg-atp-card {\n      padding: 20px;\n    }\n\n    .afg-atp-highlight,\n    .afg-atp-note {\n      padding: 16px 18px;\n    }\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"afg-atp-page\"\u003e\n\n  \u003csection class=\"afg-atp-hero\"\u003e\n    \u003cdiv class=\"afg-atp-eyebrow\"\u003eAffiASSAY® ATP Assay Kit\u003c\/div\u003e\n    \u003ch1 class=\"afg-atp-title\"\u003eATP Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/h1\u003e\n    \u003cp class=\"afg-atp-subtitle\"\u003e\n      A compact enzymatic assay for ATP quantification in biological samples, with flexible readout by absorbance\n      at OD 570 nm or fluorescence at Ex\/Em 535\/587 nm.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-badges\"\u003e\n  \u003cdiv class=\"afg-atp-badge\"\u003eCatalog No. AFG-BV-0083\u003c\/div\u003e\n  \u003cdiv class=\"afg-atp-badge\"\u003e100 wells\u003c\/div\u003e\n  \u003cdiv class=\"afg-atp-badge\"\u003eColorimetric or fluorometric\u003c\/div\u003e\n  \u003cdiv class=\"afg-atp-badge\"\u003eReduced-volume protocol\u003c\/div\u003e\n  \u003cdiv class=\"afg-atp-badge\"\u003eResearch use only\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eOverview\u003c\/h2\u003e\n    \u003cp\u003e\n      ATP is the main cellular energy molecule and is widely used as an indicator of metabolic activity,\n      mitochondrial function, cell viability, and tissue energy status. This assay generates a signal proportional\n      to the ATP amount present in the sample and can be measured using either absorbance or fluorescence detection.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-highlight\"\u003e\n  \u003cstrong\u003eDetection modes:\u003c\/strong\u003e OD 570 nm for colorimetric measurement, or Ex\/Em 535\/587 nm for\n  fluorometric measurement.\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eAssay Principle\u003c\/h2\u003e\n    \u003cp\u003e\n      ATP in the sample drives an enzymatic conversion reaction. The resulting intermediate is further processed\n      to generate hydrogen peroxide, which oxidizes a signal probe to produce a colored and fluorescent product.\n      The final signal intensity is proportional to ATP concentration.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-image\"\u003e\n  \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/atp-assay-principle.png\" alt=\"ATP assay principle showing ATP conversion and signal detection\"\u003e\n  \u003cdiv class=\"afg-atp-image-caption\"\u003eAssay principle: ATP-dependent enzymatic conversion followed by signal probe oxidation.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eKit Components\u003c\/h2\u003e\n    \u003cp\u003e\n      Component names are presented using simplified functional descriptions for a compact product page format.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eComponent\u003c\/th\u003e\n        \u003cth\u003eFunctional Name\u003c\/th\u003e\n        \u003cth\u003eSize\u003c\/th\u003e\n        \u003cth\u003eStorage\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n        \u003ctd\u003eReagent A\u003c\/td\u003e\n        \u003ctd\u003eAssay Buffer\u003c\/td\u003e\n        \u003ctd\u003e25 mL\u003c\/td\u003e\n        \u003ctd\u003e-20°C or 4°C\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd\u003eReagent B\u003c\/td\u003e\n        \u003ctd\u003eSignal Probe\u003c\/td\u003e\n        \u003ctd\u003e0.2 mL\u003c\/td\u003e\n        \u003ctd\u003e-20°C, protect from light\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd\u003eReagent C\u003c\/td\u003e\n        \u003ctd\u003eATP Conversion Enzyme\u003c\/td\u003e\n        \u003ctd\u003eLyophilized\u003c\/td\u003e\n        \u003ctd\u003e-20°C\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd\u003eReagent D\u003c\/td\u003e\n        \u003ctd\u003eSignal Development Enzyme Mix\u003c\/td\u003e\n        \u003ctd\u003eLyophilized\u003c\/td\u003e\n        \u003ctd\u003e-20°C\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd\u003eReagent E\u003c\/td\u003e\n        \u003ctd\u003eATP Calibrator\u003c\/td\u003e\n        \u003ctd\u003eLyophilized\u003c\/td\u003e\n        \u003ctd\u003e-20°C\u003c\/td\u003e\n      \u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eStorage and Handling\u003c\/h2\u003e\n    \u003cul class=\"afg-atp-list\"\u003e\n      \u003cli\u003eStore the kit at -20°C.\u003c\/li\u003e\n      \u003cli\u003eProtect light-sensitive reagents from direct light.\u003c\/li\u003e\n      \u003cli\u003eBriefly centrifuge vials before opening.\u003c\/li\u003e\n      \u003cli\u003eBring liquid reagents to room temperature before use.\u003c\/li\u003e\n      \u003cli\u003eKeep prepared samples and standards on ice when possible.\u003c\/li\u003e\n      \u003cli\u003eAvoid repeated freeze-thaw cycles of enzyme reagents.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eReagent Preparation\u003c\/h2\u003e\n\n\n\u003cdiv class=\"afg-atp-grid\"\u003e\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eReagent C — ATP Conversion Enzyme\u003c\/h3\u003e\n    \u003cp\u003e\n      Reconstitute with 220 µL Assay Buffer. Mix gently until fully dissolved. Aliquot and store at -20°C.\n    \u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eReagent D — Signal Development Enzyme Mix\u003c\/h3\u003e\n    \u003cp\u003e\n      Reconstitute with 220 µL Assay Buffer. Mix gently until fully dissolved. Aliquot and store at -20°C.\n    \u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eReagent E — ATP Calibrator\u003c\/h3\u003e\n    \u003cp\u003e\n      Reconstitute with 100 µL deionized water to prepare a concentrated ATP calibrator stock.\n      Keep on ice during use.\n    \u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eFluorometric Signal Probe Working Solution\u003c\/h3\u003e\n    \u003cp\u003e\n      For fluorometric detection, prepare a 1:10 diluted Signal Probe working solution before preparing\n      the reaction mix. This improves pipetting accuracy in reduced-volume format.\n    \u003c\/p\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eReduced-Volume Assay Protocol\u003c\/h2\u003e\n    \u003cp\u003e\n      This compact format uses 25 µL prepared standard or sample plus 25 µL reaction mix, for a final assay\n      volume of 50 µL per well.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-note\"\u003e\n  \u003cstrong\u003eFinal well volume:\u003c\/strong\u003e 25 µL standard or sample + 25 µL reaction mix = 50 µL per well.\n\u003c\/div\u003e\n\n\u003ch3\u003eSample Preparation\u003c\/h3\u003e\n\u003col class=\"afg-atp-steps\"\u003e\n  \u003cli\u003eHomogenize or rapidly disrupt approximately 1 × 10⁶ cells or 10 mg tissue.\u003c\/li\u003e\n  \u003cli\u003eUse 100 µL of a suitable protein-precipitating solution.\u003c\/li\u003e\n  \u003cli\u003eKeep samples cold during preparation.\u003c\/li\u003e\n  \u003cli\u003eCentrifuge to remove debris and precipitated proteins.\u003c\/li\u003e\n  \u003cli\u003eTransfer the clear supernatant to a clean tube.\u003c\/li\u003e\n  \u003cli\u003eUse 2–10 µL prepared sample per well.\u003c\/li\u003e\n  \u003cli\u003eAdjust each sample well to 25 µL final sample volume using Assay Buffer.\u003c\/li\u003e\n\u003c\/ol\u003e\n\n\u003cdiv class=\"afg-atp-highlight\"\u003e\n  \u003cstrong\u003eImportant:\u003c\/strong\u003e ATP is unstable. Prepare samples fresh whenever possible. If storage is required,\n  snap-freeze samples rapidly and keep frozen until analysis.\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eStandard Curve Preparation\u003c\/h2\u003e\n\n\n\u003cdiv class=\"afg-atp-grid\"\u003e\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eColorimetric Standards\u003c\/h3\u003e\n    \u003cdiv class=\"afg-atp-table-wrap\"\u003e\n      \u003ctable class=\"afg-atp-table\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth\u003eStandard\u003c\/th\u003e\n            \u003cth\u003eATP Amount per Well\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS0\u003c\/td\u003e\n\u003ctd\u003e0 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS1\u003c\/td\u003e\n\u003ctd\u003e2 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS2\u003c\/td\u003e\n\u003ctd\u003e4 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS3\u003c\/td\u003e\n\u003ctd\u003e6 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS4\u003c\/td\u003e\n\u003ctd\u003e8 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS5\u003c\/td\u003e\n\u003ctd\u003e10 nmol\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"afg-atp-card\"\u003e\n    \u003ch3\u003eFluorometric Standards\u003c\/h3\u003e\n    \u003cdiv class=\"afg-atp-table-wrap\"\u003e\n      \u003ctable class=\"afg-atp-table\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth\u003eStandard\u003c\/th\u003e\n            \u003cth\u003eATP Amount per Well\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS0\u003c\/td\u003e\n\u003ctd\u003e0 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS1\u003c\/td\u003e\n\u003ctd\u003e200 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS2\u003c\/td\u003e\n\u003ctd\u003e400 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS3\u003c\/td\u003e\n\u003ctd\u003e600 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS4\u003c\/td\u003e\n\u003ctd\u003e800 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eS5\u003c\/td\u003e\n\u003ctd\u003e1000 pmol\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eReaction Mix Preparation\u003c\/h2\u003e\n    \u003cp\u003e\n      Prepare enough reaction mix for all standards, samples, and background controls. Include at least 10%\n      extra volume to compensate for pipetting loss.\n    \u003c\/p\u003e\n\n\n\u003ch3\u003eColorimetric Reaction Mix\u003c\/h3\u003e\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eComponent\u003c\/th\u003e\n        \u003cth\u003eTest Reaction\u003c\/th\u003e\n        \u003cth\u003eBackground Control\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n\u003ctd\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd\u003e22 µL\u003c\/td\u003e\n\u003ctd\u003e23 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eSignal Probe\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eATP Conversion Enzyme\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eSignal Development Enzyme Mix\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e25 µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e25 µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eFluorometric Reaction Mix\u003c\/h3\u003e\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eComponent\u003c\/th\u003e\n        \u003cth\u003eTest Reaction\u003c\/th\u003e\n        \u003cth\u003eBackground Control\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n\u003ctd\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd\u003e22 µL\u003c\/td\u003e\n\u003ctd\u003e23 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eDiluted Signal Probe Working Solution\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eATP Conversion Enzyme\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eSignal Development Enzyme Mix\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003ctd\u003e1 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e25 µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e25 µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003ePlate Setup and Measurement\u003c\/h2\u003e\n\n\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eStep\u003c\/th\u003e\n        \u003cth\u003eVolume or Condition\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n\u003ctd\u003eStandard or sample\u003c\/td\u003e\n\u003ctd\u003e25 µL per well\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eReaction mix\u003c\/td\u003e\n\u003ctd\u003e25 µL per well\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eFinal well volume\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eIncubation\u003c\/td\u003e\n\u003ctd\u003eRoom temperature, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eColorimetric reading\u003c\/td\u003e\n\u003ctd\u003eOD 570 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003eFluorometric reading\u003c\/td\u003e\n\u003ctd\u003eEx\/Em 535\/587 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp\u003e\n  Endpoint signal is typically reached within 30 minutes. Use the same incubation time for standards,\n  samples, and controls.\n\u003c\/p\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eTypical Colorimetric Results\u003c\/h2\u003e\n    \u003cp\u003e\n      Representative colorimetric standard curve using ATP standards from 0 to 10 nmol per well.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-image\"\u003e\n  \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/atp-assay-colorimetry.png\" alt=\"ATP colorimetric standard curve OD 570 nm\"\u003e\n  \u003cdiv class=\"afg-atp-image-caption\"\u003eColorimetric ATP standard curve, OD 570 nm.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eATP Standard\u003c\/th\u003e\n        \u003cth\u003eRaw OD 1\u003c\/th\u003e\n        \u003cth\u003eRaw OD 2\u003c\/th\u003e\n        \u003cth\u003eCorrected OD 1\u003c\/th\u003e\n        \u003cth\u003eCorrected OD 2\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n\u003ctd\u003e0 nmol\u003c\/td\u003e\n\u003ctd\u003e0.041\u003c\/td\u003e\n\u003ctd\u003e0.043\u003c\/td\u003e\n\u003ctd\u003e0.000\u003c\/td\u003e\n\u003ctd\u003e0.000\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e2 nmol\u003c\/td\u003e\n\u003ctd\u003e0.292\u003c\/td\u003e\n\u003ctd\u003e0.286\u003c\/td\u003e\n\u003ctd\u003e0.251\u003c\/td\u003e\n\u003ctd\u003e0.243\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e4 nmol\u003c\/td\u003e\n\u003ctd\u003e0.548\u003c\/td\u003e\n\u003ctd\u003e0.556\u003c\/td\u003e\n\u003ctd\u003e0.507\u003c\/td\u003e\n\u003ctd\u003e0.513\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e6 nmol\u003c\/td\u003e\n\u003ctd\u003e0.806\u003c\/td\u003e\n\u003ctd\u003e0.824\u003c\/td\u003e\n\u003ctd\u003e0.765\u003c\/td\u003e\n\u003ctd\u003e0.781\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e8 nmol\u003c\/td\u003e\n\u003ctd\u003e1.072\u003c\/td\u003e\n\u003ctd\u003e1.061\u003c\/td\u003e\n\u003ctd\u003e1.031\u003c\/td\u003e\n\u003ctd\u003e1.018\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e10 nmol\u003c\/td\u003e\n\u003ctd\u003e1.329\u003c\/td\u003e\n\u003ctd\u003e1.345\u003c\/td\u003e\n\u003ctd\u003e1.288\u003c\/td\u003e\n\u003ctd\u003e1.302\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  Typical linear fit: OD 570 nm = 0.129 × ATP nmol + 0.002\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eTypical Fluorometric Results\u003c\/h2\u003e\n    \u003cp\u003e\n      Representative fluorometric standard curve using ATP standards from 0 to 1000 pmol per well.\n    \u003c\/p\u003e\n\n\n\u003cdiv class=\"afg-atp-image\"\u003e\n  \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/atp-assay-fluorometry.png\" alt=\"ATP fluorometric standard curve Ex Em 535 587 nm\"\u003e\n  \u003cdiv class=\"afg-atp-image-caption\"\u003eFluorometric ATP standard curve, Ex\/Em 535\/587 nm.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"afg-atp-table-wrap\"\u003e\n  \u003ctable class=\"afg-atp-table\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth\u003eATP Standard\u003c\/th\u003e\n        \u003cth\u003eRaw RFU 1\u003c\/th\u003e\n        \u003cth\u003eRaw RFU 2\u003c\/th\u003e\n        \u003cth\u003eCorrected RFU 1\u003c\/th\u003e\n        \u003cth\u003eCorrected RFU 2\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n\u003ctd\u003e0 pmol\u003c\/td\u003e\n\u003ctd\u003e215\u003c\/td\u003e\n\u003ctd\u003e228\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e200 pmol\u003c\/td\u003e\n\u003ctd\u003e2,840\u003c\/td\u003e\n\u003ctd\u003e2,910\u003c\/td\u003e\n\u003ctd\u003e2,625\u003c\/td\u003e\n\u003ctd\u003e2,682\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e400 pmol\u003c\/td\u003e\n\u003ctd\u003e5,520\u003c\/td\u003e\n\u003ctd\u003e5,610\u003c\/td\u003e\n\u003ctd\u003e5,305\u003c\/td\u003e\n\u003ctd\u003e5,382\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e600 pmol\u003c\/td\u003e\n\u003ctd\u003e8,230\u003c\/td\u003e\n\u003ctd\u003e8,145\u003c\/td\u003e\n\u003ctd\u003e8,015\u003c\/td\u003e\n\u003ctd\u003e7,917\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e800 pmol\u003c\/td\u003e\n\u003ctd\u003e10,780\u003c\/td\u003e\n\u003ctd\u003e10,940\u003c\/td\u003e\n\u003ctd\u003e10,565\u003c\/td\u003e\n\u003ctd\u003e10,712\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003ctr\u003e\n\u003ctd\u003e1000 pmol\u003c\/td\u003e\n\u003ctd\u003e13,420\u003c\/td\u003e\n\u003ctd\u003e13,310\u003c\/td\u003e\n\u003ctd\u003e13,205\u003c\/td\u003e\n\u003ctd\u003e13,082\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  Typical linear fit: RFU = 13.2 × ATP pmol + 18\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eCalculation\u003c\/h2\u003e\n\n\n\u003col class=\"afg-atp-steps\"\u003e\n  \u003cli\u003eSubtract the blank value from all standards.\u003c\/li\u003e\n  \u003cli\u003ePlot corrected signal versus ATP amount.\u003c\/li\u003e\n  \u003cli\u003eDetermine the slope of the standard curve.\u003c\/li\u003e\n  \u003cli\u003eFor each sample, subtract the paired background control.\u003c\/li\u003e\n  \u003cli\u003eUse the standard curve to calculate ATP amount in the well.\u003c\/li\u003e\n\u003c\/ol\u003e\n\n\u003ch3\u003eATP concentration in loaded sample\u003c\/h3\u003e\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  ATP per µL sample = ATP amount in well ÷ sample volume added\n\u003c\/div\u003e\n\n\u003ch3\u003eTotal ATP in original sample\u003c\/h3\u003e\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  Total ATP = ATP per µL sample × total recovered sample volume\n\u003c\/div\u003e\n\n\u003ch3\u003eNormalized ATP content\u003c\/h3\u003e\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  ATP per mg tissue = total ATP ÷ tissue mass\n\u003c\/div\u003e\n\n\u003cdiv class=\"afg-atp-formula\"\u003e\n  ATP per cell number = total ATP ÷ number of cells\n\u003c\/div\u003e\n\n\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-section\"\u003e\n    \u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n    \u003cul class=\"afg-atp-list\"\u003e\n      \u003cli\u003eUse fresh samples whenever possible.\u003c\/li\u003e\n      \u003cli\u003eKeep samples cold during extraction.\u003c\/li\u003e\n      \u003cli\u003ePrepare paired background controls for each sample type.\u003c\/li\u003e\n      \u003cli\u003eProtect detection reagents from light.\u003c\/li\u003e\n      \u003cli\u003eUse the same incubation time for standards and samples.\u003c\/li\u003e\n      \u003cli\u003eVerify linearity when using the reduced-volume format.\u003c\/li\u003e\n      \u003cli\u003eDo not compare colorimetric and fluorometric results without separate standard curves.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/section\u003e\n\n  \u003csection class=\"afg-atp-cta\"\u003e\n    \u003ch2\u003eReliable ATP Quantification in a Compact Format\u003c\/h2\u003e\n    \u003cp\u003e\n      The AffiASSAY® ATP Colorimetric \u0026amp; Fluorometric Assay Kit combines flexible detection, simplified\n      reagent naming, reduced reaction volume, paired background correction, and clear standard-curve-based\n      quantification for routine ATP analysis in research samples.\n    \u003c\/p\u003e\n  \u003c\/section\u003e\n\n\u003c\/div\u003e\n","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294222598475,"sku":"AFG-BV-0083","price":420.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_afcc562c-4d1e-43bc-8791-20a8269254c7.png?v=1705255849"},{"product_id":"affiassay-acetate-colorimetric-assay-kit","title":"AffiASSAY® Acetate Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan data-mce-fragment=\"1\" style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Acetate Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Acetate Colorimetric Assay Kit is designed to provide precise and reliable measurements of Acetate levels in a variety of biological samples. This assay kit is a powerful tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of Acetate, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Acetate Colorimetric Assay Kit can be used with a broad range of sample types, including cells, tissues, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cellular metabolism, investigating the effects of drug compounds, or exploring the intricacies of enzymatic reactions, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of Acetate into a colored product. The increase in color intensity is directly proportional to the Acetate concentration in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Research:\u003c\/strong\u003e Study the role of Acetate in cellular energy production and lipid metabolism.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Development:\u003c\/strong\u003e Assess the impact of pharmaceutical compounds on Acetate levels, aiding in drug discovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Kinetics:\u003c\/strong\u003e Investigate the kinetics of Acetate-utilizing enzymes with accuracy and ease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Explore the connections between Acetate and cellular signaling pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eAcetate Standard\u003c\/li\u003e\n\u003cli\u003eEnzyme Mix\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Acetate Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Acetate Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing Acetate\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at appropriate wavelength\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing Acetate. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Acetate Standard provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Acetate standard to generate a standard curve. Typical concentrations may range from 0 to 100 µM.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a control sample without Acetate to serve as a background control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample Dilution:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDilute your biological samples as needed to ensure that they fall within the linear range of the standard curve. The specific dilution factor will depend on the expected Acetate concentration in your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer and Enzyme Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your diluted samples and Acetate standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic conversion of Acetate to a colored product.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAbsorbance Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring absorbance at the appropriate wavelength, measure the absorbance of each well. The specific wavelength for measurement will depend on the colorimetric detection method used.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the Acetate standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the Acetate concentrations in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in Acetate levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Acetate Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289379659,"sku":"AFG-BV-0014","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_96628ec4-f4f4-49be-91a8-b53e1dcbbed9.png?v=1705258034"},{"product_id":"affiassay-alanine-aminotransferase-alt-or-sgpt-activity-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026amp; Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of ALT activity in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ALT activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric and Fluorometric Detection:\u003c\/strong\u003e With both colorimetric and fluorometric detection options, our kit ensures flexibility and convenience, catering to different experimental needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying liver function, investigating disease mechanisms, or screening potential therapeutics, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric or fluorometric method based on the enzymatic conversion of alanine and α-ketoglutarate into pyruvate and glutamate by ALT. The generated product can be detected either through colorimetric changes or fluorescence emission, allowing for precise quantification of ALT activity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLiver Function Studies:\u003c\/strong\u003e Assess ALT activity as a biomarker of liver health and function.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisease Mechanism Investigations:\u003c\/strong\u003e Investigate the role of ALT in various diseases, including liver diseases and metabolic disorders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential therapeutics for their impact on ALT activity, aiding in drug development.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Metabolism:\u003c\/strong\u003e Study the involvement of ALT in cellular metabolism and amino acid metabolism.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eALT Enzyme\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003eSubstrate Mix\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ALT\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ALT. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known ALT activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, ALT Enzyme, and Substrate Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring absorbance or fluorescence, measure the absorbance or fluorescence intensity of each well.\u003c\/li\u003e\n\u003cli\u003eFor colorimetric detection, measure the absorbance at the appropriate wavelength.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection, measure the fluorescence emission at the appropriate excitation and emission wavelengths.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ALT activity in your samples based on their respective absorbance or fluorescence readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ALT activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289412427,"sku":"AFG-BV-0041","price":396.63,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_b664a8d1-d166-48f5-aedd-c6d84732407c.png?v=1705258037"},{"product_id":"affiassay-alcohol-dehydrogenase-activity-colorimetric-assay-kit","title":"AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan data-mce-fragment=\"1\" style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of alcohol dehydrogenase (ADH) activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ADH activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying alcohol metabolism, investigating the effects of alcohol on cellular pathways, or screening potential inhibitors, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of alcohol into its corresponding aldehyde by ADH, coupled with the reduction of NAD\u003csup\u003e+\u003c\/sup\u003e to NADH. The increase in absorbance at 450 nm is directly proportional to the ADH activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlcohol Metabolism Studies:\u003c\/strong\u003e Assess ADH activity as a key enzyme involved in alcohol metabolism and detoxification processes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential inhibitors or activators of ADH for their pharmacological effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Investigate the role of ADH in cellular signaling pathways and its implications in various physiological processes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eNAD\u003csup\u003e+\u003c\/sup\u003e (Nicotinamide Adenine Dinucleotide)\u003c\/li\u003e\n\u003cli\u003eAlcohol Substrate\u003c\/li\u003e\n\u003cli\u003eADH Enzyme\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ADH\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ADH. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known ADH activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, NAD\u003csup\u003e+\u003c\/sup\u003e, Alcohol Substrate, and ADH Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAbsorbance Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring absorbance at 450 nm, measure the absorbance of each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ADH activity in your samples based on their respective absorbance readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ADH activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289477963,"sku":"AFG-BV-0045","price":413.73,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_ee63cc80-c661-4f64-b29f-ec05a647c9df.png?v=1705258039"},{"product_id":"affiassay-aldehyde-dehydrogenase-activity-colorimetric-assay-kit","title":"AffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of aldehyde dehydrogenase (ALDH) activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ALDH activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cellular detoxification processes, investigating the effects of ALDH inhibitors, or screening potential therapeutic compounds, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of aldehydes into their corresponding carboxylic acids by ALDH, coupled with the reduction of NAD\u003csup\u003e+\u003c\/sup\u003e to NADH. The increase in absorbance at 450 nm is directly proportional to the ALDH activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetoxification Studies:\u003c\/strong\u003e Assess ALDH activity as a key enzyme involved in cellular detoxification processes, particularly in the metabolism of reactive aldehydes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential ALDH inhibitors or activators for their pharmacological effects, particularly in the context of diseases associated with oxidative stress and aldehyde toxicity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Investigate the role of ALDH in cellular signaling pathways and its implications in various physiological processes, including cell proliferation and apoptosis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eNAD\u003csup\u003e+\u003c\/sup\u003e (Nicotinamide Adenine Dinucleotide)\u003c\/li\u003e\n\u003cli\u003eAldehyde Substrate\u003c\/li\u003e\n\u003cli\u003eALDH Enzyme\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ALDH\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ALDH. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known ALDH activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, NAD\u003csup\u003e+\u003c\/sup\u003e, Aldehyde Substrate, and ALDH Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAbsorbance Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring absorbance at 450 nm, measure the absorbance of each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ALDH activity in your samples based on their respective absorbance readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ALDH activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Aldehyde Dehydrogenase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289543499,"sku":"AFG-BV-0046","price":405.18,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_56c77f94-3b6b-4716-85aa-f518e6423636.png?v=1705258042"},{"product_id":"affiassay-aldehyde-dehydrogenase-activity-fluorometric-assay-kit","title":"AffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit is designed to provide precise and reliable measurements of aldehyde dehydrogenase (ALDH) activity in biological samples. This assay kit offers a fluorometric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ALDH activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cellular detoxification processes, investigating the effects of ALDH inhibitors, or screening potential therapeutic compounds, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a fluorometric method based on the enzymatic conversion of aldehydes into their corresponding carboxylic acids by ALDH, coupled with the production of NADH. The increase in fluorescence intensity at specific wavelengths is directly proportional to the ALDH activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetoxification Studies:\u003c\/strong\u003e Assess ALDH activity as a key enzyme involved in cellular detoxification processes, particularly in the metabolism of reactive aldehydes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential ALDH inhibitors or activators for their pharmacological effects, particularly in the context of diseases associated with oxidative stress and aldehyde toxicity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Investigate the role of ALDH in cellular signaling pathways and its implications in various physiological processes, including cell proliferation and apoptosis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eNAD\u003csup\u003e+\u003c\/sup\u003e (Nicotinamide Adenine Dinucleotide)\u003c\/li\u003e\n\u003cli\u003eAldehyde Substrate\u003c\/li\u003e\n\u003cli\u003eALDH Enzyme\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ALDH\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ALDH. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known ALDH activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, NAD\u003csup\u003e+\u003c\/sup\u003e, Aldehyde Substrate, and ALDH Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring fluorescence intensity at specific wavelengths, measure the fluorescence intensity of each well.\u003c\/li\u003e\n\u003cli\u003eRecord the fluorescence emission at the appropriate excitation and emission wavelengths.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ALDH activity in your samples based on their respective fluorescence intensity readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ALDH activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Aldehyde Dehydrogenase Activity Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289576267,"sku":"AFG-BV-0047","price":341.05,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_c7ba8957-b19a-43f1-b37c-4a934f233d47.png?v=1705258044"},{"product_id":"affiassay-alkaline-phosphatase-activity-colorimetric-assay-kit","title":"AffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of alkaline phosphatase (ALP) activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ALP activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying bone metabolism, investigating ALP as a biomarker in disease, or screening for ALP inhibitors, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic hydrolysis of a phosphate ester substrate by ALP, leading to the production of a chromogenic product. The increase in absorbance at a specific wavelength is directly proportional to the ALP activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBone Metabolism Studies:\u003c\/strong\u003e Assess ALP activity as a marker of bone turnover and mineralization processes, particularly in osteoblast differentiation and bone formation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Diagnostics:\u003c\/strong\u003e Measure ALP activity in serum or plasma samples to diagnose liver or bone disorders, such as liver disease or bone fractures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential ALP inhibitors or activators for their pharmacological effects, particularly in the context of diseases associated with abnormal ALP activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003ePhosphate Ester Substrate\u003c\/li\u003e\n\u003cli\u003eALP Enzyme\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003ep-Nitrophenol Standard\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ALP\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ALP. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known ALP activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, Phosphate Ester Substrate, and ALP Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAbsorbance Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a microplate reader capable of measuring absorbance at a specific wavelength (typically around 405 nm), measure the absorbance of each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ALP activity in your samples based on their respective absorbance readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ALP activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Alkaline Phosphatase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"500 Assays","offer_id":47294289609035,"sku":"AFG-BV-0050","price":370.98,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_aa799533-6c52-4fe5-ab9c-f8ded261986e.png?v=1705258047"},{"product_id":"affiassay-alpha-galactosidase-α-gal-activity-fluorometric-assay-kit","title":"AffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit is designed to provide precise and reliable measurements of alpha galactosidase activity in biological samples. This assay kit offers a fluorometric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of α-Gal activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Alpha Galactosidase Activity Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying lysosomal storage disorders, investigating carbohydrate metabolism, or screening potential therapeutic compounds, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a fluorometric method based on the enzymatic hydrolysis of a synthetic fluorogenic substrate by α-Gal, leading to the release of a fluorescent product. The increase in fluorescence intensity at specific wavelengths is directly proportional to the α-Gal activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLysosomal Storage Disorders:\u003c\/strong\u003e Measure α-Gal activity as a diagnostic marker for lysosomal storage disorders, such as Fabry disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCarbohydrate Metabolism:\u003c\/strong\u003e Study the role of α-Gal in carbohydrate metabolism and glycosphingolipid degradation pathways.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential α-Gal inhibitors or activators for their pharmacological effects, particularly in the context of lysosomal storage disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eFluorogenic Substrate\u003c\/li\u003e\n\u003cli\u003eα-Gal Enzyme\u003c\/li\u003e\n\u003cli\u003ePositive Control\u003c\/li\u003e\n\u003cli\u003eFluorescence Enhancer\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing α-Gal\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eFluorescence microplate reader capable of measuring fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing α-Gal. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare a positive control sample with known α-Gal activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, Fluorogenic Substrate, α-Gal Enzyme, and Fluorescence Enhancer from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUsing a fluorescence microplate reader capable of measuring fluorescence intensity at specific wavelengths (excitation and emission wavelengths provided by the kit), measure the fluorescence intensity of each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the α-Gal activity in your samples based on their respective fluorescence intensity readings.\u003c\/li\u003e\n\u003cli\u003eCompare the activity levels to your positive control and perform any necessary normalization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in α-Gal activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Alpha Galactosidase (α-Gal) Activity Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289641803,"sku":"AFG-BV-0053","price":370.98,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_ccb7771b-a2b6-4bc8-b151-b2f903405dce.png?v=1705258050"},{"product_id":"affiassay-alpha-ketoglutarate-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of alpha-ketoglutarate (α-KG) levels in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of α-KG, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric and Fluorometric Detection:\u003c\/strong\u003e With dual detection options, our kit provides flexibility and versatility to meet your experimental needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRobust Performance:\u003c\/strong\u003e Our kit ensures robust and reproducible results, minimizing the margin for error and enhancing the reliability of your data.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a method based on the enzymatic conversion of α-KG into a product that reacts with a probe to generate a colorimetric or fluorometric signal. The intensity of the signal is directly proportional to the α-KG concentration in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Research:\u003c\/strong\u003e Study the role of α-KG in various metabolic pathways, including the tricarboxylic acid (TCA) cycle and amino acid metabolism.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Signaling:\u003c\/strong\u003e Investigate the involvement of α-KG in cellular signaling pathways, such as hypoxia-inducible factor (HIF) regulation and epigenetic modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisease Biomarker:\u003c\/strong\u003e Assess α-KG levels as a potential biomarker for diseases such as cancer, metabolic disorders, and neurodegenerative diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eα-Ketoglutarate Probe\u003c\/li\u003e\n\u003cli\u003eα-Ketoglutarate Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing α-KG\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing α-KG. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known α-KG concentrations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, α-Ketoglutarate Probe, and α-Ketoglutarate Enzyme Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFor colorimetric detection: Measure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection: Measure the fluorescence intensity of each well using a fluorescence microplate reader with appropriate excitation and emission wavelengths (provided by the kit).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the α-KG concentration in your samples based on their respective absorbance or fluorescence intensity readings.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in α-KG levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Alpha-Ketoglutarate Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289674571,"sku":"AFG-BV-0054","price":375.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_45a1705f-8d6e-43c9-893a-d7b4164f4fe8.png?v=1705258052"},{"product_id":"affiassay-ammonia-colorimetric-assay-kit","title":"AffiASSAY® Ammonia Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Ammonia Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Ammonia Colorimetric Assay Kit is designed to provide precise and reliable measurements of ammonia levels in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ammonia, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Ammonia Colorimetric Assay Kit can be used with various sample types, including cell culture supernatants, serum, plasma, and urine.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying nitrogen metabolism, monitoring ammonia levels in clinical samples, or assessing environmental ammonia pollution, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of ammonia into a product that reacts with a probe to generate a colored compound. The intensity of the color is directly proportional to the ammonia concentration in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNitrogen Metabolism:\u003c\/strong\u003e Study the role of ammonia in nitrogen metabolism and urea cycle disorders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Diagnostics:\u003c\/strong\u003e Measure ammonia levels in serum or plasma samples to diagnose liver diseases, such as hepatic encephalopathy.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnvironmental Monitoring:\u003c\/strong\u003e Assess ammonia pollution levels in water or soil samples for environmental monitoring and assessment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eAmmonia Probe\u003c\/li\u003e\n\u003cli\u003eAmmonia Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Ammonia Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Ammonia Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ammonia\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ammonia. These samples can be cell culture supernatants, serum, plasma, urine, or other relevant biological fluids.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known ammonia concentrations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, Ammonia Probe, and Ammonia Enzyme Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ammonia concentration in your samples based on their respective absorbance readings and compare them to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ammonia levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Ammonia Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289707339,"sku":"AFG-BV-0058","price":319.68,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_9a9f3c89-19c7-4252-8649-2b6489cbb6a7.png?v=1705258055"},{"product_id":"affiassay-ammonia-colorimetric-assay-kit-v2","title":"AffiASSAY® Ammonia Colorimetric Assay Kit V2","description":"\u003ch2\u003e\n \u003cmeta charset=\"utf-8\"\u003e \u003cspan data-mce-fragment=\"1\" style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Ammonia Colorimetric Assay Kit V2\u003c\/span\u003e\n\u003c\/h2\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289740107,"sku":"AFG-BV-0702","price":358.15,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_879a1c48-8800-49e1-b9ed-e8c0ef33a01f.png?v=1705258058"},{"product_id":"affiassay-amylase-activity-colorimetric-assay-kit","title":"AffiASSAY® Amylase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Amylase Activity Colorimetric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Amylase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of amylase activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, enzymology, and food science.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of amylase activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Amylase Activity Colorimetric Assay Kit can be used with various sample types, including saliva, serum, plasma, urine, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying carbohydrate metabolism, assessing digestive enzyme activity, or screening potential inhibitors or activators of amylase, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic hydrolysis of a starch substrate by amylase, leading to the release of a colored product. The intensity of the color is directly proportional to the amylase activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCarbohydrate Metabolism:\u003c\/strong\u003e Study the role of amylase in carbohydrate digestion and metabolism, particularly in the context of diabetes and other metabolic disorders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDigestive Enzyme Analysis:\u003c\/strong\u003e Assess amylase activity in biological fluids, such as saliva and serum, to evaluate pancreatic function and diagnose related disorders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen potential amylase inhibitors or activators for their pharmacological effects, aiding in drug discovery and development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eStarch Substrate\u003c\/li\u003e\n\u003cli\u003eAmylase Enzyme\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Amylase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Amylase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing amylase\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing amylase. These samples can be saliva, serum, plasma, urine, or culture supernatants.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known amylase activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer and Starch Substrate from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the amylase activity in your samples based on their respective absorbance readings and compare them to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in amylase activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Amylase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289772875,"sku":"AFG-BV-0061","price":289.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_362e3a09-d963-44bd-878e-3d5432e617c1.png?v=1705258060"},{"product_id":"affiassay-angiotensin-ii-converting-enzyme-ace2-activity-fluorometric-assay-kit","title":"AffiASSAY® Angiotensin II Converting Enzyme (ACE2) Activity Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Angiotensin II Converting Enzyme (ACE2) Activity Fluorometric Assay Kit \u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Angiotensin II Converting Enzyme (ACE2) Activity Fluorometric Assay Kit is designed to provide precise and reliable measurements of ACE2 enzyme activity in biological samples. This assay kit offers a fluorometric detection method, making it a powerful tool for researchers in the fields of biochemistry, molecular biology, and pharmacology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of ACE2 activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® ACE2 Activity Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, serum, plasma, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying the renin-angiotensin system, investigating the role of ACE2 in viral infections, or screening potential ACE2 inhibitors or activators, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a fluorometric method based on the enzymatic conversion of a specific ACE2 substrate into a fluorescent product. The increase in fluorescence intensity is directly proportional to the ACE2 activity in your sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRenin-Angiotensin System:\u003c\/strong\u003e Study the role of ACE2 in the renin-angiotensin system and its implications in cardiovascular health and disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eViral Infections:\u003c\/strong\u003e Investigate the involvement of ACE2 in viral infections, particularly in the context of coronavirus entry into host cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen potential ACE2 inhibitors or activators for their pharmacological effects, aiding in drug development for cardiovascular and infectious diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eACE2 Substrate\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003eACE2 Enzyme\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® ACE2 Activity Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® ACE2 Activity Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ACE2\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eFluorescence microplate reader capable of measuring fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing ACE2. These samples can be cell lysates, tissue homogenates, serum, plasma, or culture supernatants.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known ACE2 activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, ACE2 Substrate, and ACE2 Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the fluorescence intensity of each well using a fluorescence microplate reader with appropriate excitation and emission wavelengths (provided by the kit).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the ACE2 activity in your samples based on their respective fluorescence intensity readings and compare them to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in ACE2 activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® ACE2 Activity Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289838411,"sku":"AFG-BV-0064","price":319.68,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_a2c5cd43-afb4-4a73-8a12-0d9ac220a4fe.png?v=1705258063"},{"product_id":"affiassay-asparaginase-activity-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Asparaginase Activity Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of asparaginase enzyme activity in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, providing flexibility for researchers in biochemistry, enzymology, and cancer research.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of asparaginase activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric and Fluorometric Detection:\u003c\/strong\u003e Choose between colorimetric or fluorometric detection methods based on your experimental needs, ensuring robust and reproducible results.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, serum, plasma, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cancer metabolism, evaluating asparaginase therapy efficacy, or screening potential inhibitors or activators of asparaginase, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs both colorimetric and fluorometric methods based on the enzymatic conversion of asparagine into aspartic acid and ammonia by asparaginase. The release of ammonia can be detected either by a colorimetric reaction or by measuring the fluorescence of a fluorogenic probe, allowing for precise quantification of asparaginase activity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCancer Metabolism:\u003c\/strong\u003e Study the role of asparaginase in cancer metabolism, particularly in the context of asparagine dependency in certain types of cancer cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTherapeutic Monitoring:\u003c\/strong\u003e Assess asparaginase activity in patient samples to monitor the efficacy of asparaginase therapy in leukemia and other malignancies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen potential asparaginase inhibitors or activators for their pharmacological effects, aiding in drug development for cancer treatment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eAsparagine Substrate\u003c\/li\u003e\n\u003cli\u003eAsparaginase Enzyme\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003eFluorogenic Probe (for fluorometric detection)\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing asparaginase\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths (for colorimetric detection) and fluorescence intensity (for fluorometric detection)\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing asparaginase. These samples can be cell lysates, tissue homogenates, serum, plasma, or culture supernatants.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known asparaginase activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer, Asparagine Substrate, and Asparaginase Enzyme from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFor Colorimetric Detection: Measure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor Fluorometric Detection: Measure the fluorescence intensity of each well using a fluorescence microplate reader with appropriate excitation and emission wavelengths (provided by the kit).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the asparaginase activity in your samples based on their respective absorbance readings (for colorimetric detection) or fluorescence intensity readings (for fluorometric detection) and compare them to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in asparaginase activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Asparaginase Activity Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289871179,"sku":"AFG-BV-0076","price":379.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_a8075c56-1962-4c57-93b6-0223736418cf.png?v=1705258065"},{"product_id":"affiassay-aspartate-aminotransferase-ast-or-sgot-activity-colorimetric-assay-kit","title":"AffiASSAY® Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of AST enzyme activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of clinical biochemistry, hepatology, and toxicology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of AST activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® AST Activity Colorimetric Assay Kit can be used with various sample types, including serum, plasma, cell lysates, tissue homogenates, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying liver function, assessing drug-induced hepatotoxicity, or evaluating the impact of toxins on cellular metabolism, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of aspartate and α-ketoglutarate into oxaloacetate and glutamate by AST. The generated oxaloacetate further reacts with a substrate to produce a colored product, which can be quantified spectrophotometrically.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLiver Function Assessment:\u003c\/strong\u003e Measure AST activity in serum or plasma samples to evaluate liver health and diagnose liver diseases, such as hepatitis, cirrhosis, and fatty liver disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eToxicology Studies:\u003c\/strong\u003e Assess the impact of drugs, chemicals, or environmental toxins on liver function by monitoring changes in AST activity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Research:\u003c\/strong\u003e Investigate the association between AST levels and various disease states, including cardiovascular diseases, skeletal muscle disorders, and renal diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eAST Substrate Mix\u003c\/li\u003e\n\u003cli\u003eDeveloper Solution\u003c\/li\u003e\n\u003cli\u003eStop Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® AST Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® AST Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing AST\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing AST. These samples can be serum, plasma, cell lysates, tissue homogenates, or culture supernatants.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples with known AST activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer and AST Substrate Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd 50-100 µL of the assay reaction mix to each well of the microplate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and control samples to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the AST activity in your samples based on their respective absorbance readings and compare them to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in AST activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® AST Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289903947,"sku":"AFG-BV-0078","price":370.98,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_11ce9e7a-4f17-4a11-81c5-a63be77e936e.png?v=1705258068"},{"product_id":"affiassay-branched-chain-amino-acid-leu-ile-val-colorimetric-assay-kit","title":"AffiASSAY® Branched Chain Amino Acid (Leu\/Ile\/Val) Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Branched Chain Amino Acid (Leu\/Ile\/Val) Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Branched Chain Amino Acid (Leu\/Ile\/Val) Colorimetric Assay Kit is designed to provide precise and reliable measurements of branched-chain amino acids (BCAAs) in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of nutrition, metabolism, and clinical biochemistry.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of BCAAs, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit can be used with various sample types, including serum, plasma, cell lysates, tissue homogenates, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying amino acid metabolism, assessing nutritional status, or investigating the role of BCAAs in disease states, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the enzymatic conversion of BCAAs into a product that reacts with a chromogenic reagent to produce a colored compound. The intensity of the color is directly proportional to the concentration of BCAAs in the sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNutritional Studies:\u003c\/strong\u003e Measure BCAA levels in serum or plasma samples to assess dietary intake, protein metabolism, and nutritional status.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Research:\u003c\/strong\u003e Investigate the role of BCAAs in energy metabolism, protein synthesis, and muscle function.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Biochemistry:\u003c\/strong\u003e Monitor changes in BCAA levels associated with metabolic disorders, liver diseases, and muscle wasting conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eBCAA Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eChromogenic Reagent\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing BCAAs\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your biological samples containing BCAAs. These samples can be serum, plasma, cell lysates, tissue homogenates, or culture supernatants.\u003c\/li\u003e\n\u003cli\u003eEnsure that your samples are stored and handled according to your specific experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without BCAAs to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Control Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and BCAA standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the assay reaction mix by combining the Assay Buffer and BCAA Enzyme Mix from the kit as instructed.\u003c\/li\u003e\n\u003cli\u003eAdd the assay reaction mix to each well of the microplate containing samples and standards.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eIncubation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic conversion of BCAAs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColor Development:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd the Chromogenic Reagent to each well to initiate the color development reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate for a specified time to allow the color to develop.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the BCAA standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the BCAA concentrations in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in BCAA levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289936715,"sku":"AFG-BV-0101","price":400.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_10d807e8-5534-4754-ad31-6dfbb866e758.png?v=1705258070"},{"product_id":"affiassay-calcium-colorimetric-assay-kit","title":"AffiASSAY® Calcium Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Calcium Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Calcium Colorimetric Assay Kit is designed to provide precise and reliable measurements of calcium levels in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of physiology, cell biology, and calcium signaling.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect even low levels of calcium, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Calcium Colorimetric Assay Kit can be used with various sample types, including serum, plasma, cell lysates, tissue homogenates, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying calcium homeostasis, investigating calcium-dependent signaling pathways, or evaluating the effects of calcium-modulating agents, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit employs a colorimetric method based on the reaction between calcium ions and a chromogenic reagent. The formation of a colored complex is directly proportional to the concentration of calcium in the sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Physiology:\u003c\/strong\u003e Measure calcium levels in cell lysates or culture supernatants to assess intracellular calcium signaling and calcium-dependent processes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMedical Research:\u003c\/strong\u003e Investigate the role of calcium in various physiological processes, such as muscle contraction, neurotransmitter release, and enzyme activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen compounds for their effects on calcium levels and calcium-dependent pathways, aiding in the development of novel therapeutics for diseases associated with calcium dysregulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eCalcium Chromogenic Reagent\u003c\/li\u003e\n\u003cli\u003eCalcium Standard\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Calcium Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Calcium Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing calcium\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Calcium Standard provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Calcium Standard to generate a standard curve. Typical concentrations may range from 0 to 10 mM.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and blanks.\u003c\/li\u003e\n\u003cli\u003ePrepare blank wells containing assay buffer only.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and Calcium Standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCalcium Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd the Calcium Chromogenic Reagent to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eMix the contents gently and incubate the microplate at room temperature for a specified time to allow color development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the Calcium Standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the calcium concentrations in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in calcium levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Calcium Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294289969483,"sku":"AFG-BV-0106","price":289.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_314ae070-d100-4698-961e-bfa17080df14.png?v=1705258073"},{"product_id":"affiassay-catalase-activity-colorimetric-assay-kit","title":"AffiASSAY® Catalase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Catalase Activity Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Catalase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of catalase enzyme activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and oxidative stress research.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect catalase activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Catalase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, blood, serum, plasma, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying oxidative stress, assessing antioxidant enzyme activity, or investigating the effects of drug compounds, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures catalase activity based on the decomposition of hydrogen peroxide (H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e) by catalase enzyme. The reaction produces water and oxygen, and the decrease in H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e concentration is monitored by the decrease in absorbance at a specific wavelength, allowing for the quantification of catalase activity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eOxidative Stress Research:\u003c\/strong\u003e Assess catalase activity as part of oxidative stress studies to understand the balance between reactive oxygen species (ROS) production and antioxidant defense mechanisms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Biology:\u003c\/strong\u003e Measure catalase activity in cell lysates or culture supernatants to evaluate cellular antioxidant capacity and response to oxidative insults.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiomedical Research:\u003c\/strong\u003e Investigate the role of catalase in various physiological processes, including inflammation, aging, and disease states such as cancer and neurodegenerative disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eCatalase Substrate (Hydrogen Peroxide)\u003c\/li\u003e\n\u003cli\u003eCatalase Standard\u003c\/li\u003e\n\u003cli\u003ePotassium Hydroxide (KOH)\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Catalase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Catalase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing catalase\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Catalase Standard provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Catalase Standard to generate a standard curve. Typical concentrations may range from 0 to 1000 U\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and blanks.\u003c\/li\u003e\n\u003cli\u003ePrepare blank wells containing assay buffer only.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and Catalase Standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eReaction Initiation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Catalase Substrate (Hydrogen Peroxide) to each well to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eReaction Termination:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Potassium Hydroxide (KOH) to each well after a specified incubation period to stop the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eMeasurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the Catalase Standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the catalase activity in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eReport catalase activity as units per milliliter (U\/mL) or units per milligram of protein (U\/mg protein) if protein normalization is performed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Catalase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290002251,"sku":"AFG-BV-0189","price":311.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_259d865e-64eb-4b97-849f-03b62774a4ef.png?v=1705258076"},{"product_id":"affiassay-cathepsin-b-activity-fluorometric-assay-kit","title":"AffiASSAY® Cathepsin B Activity Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cathepsin B Activity Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Cathepsin B Activity Fluorometric Assay Kit is designed to provide precise and reliable measurements of cathepsin B enzyme activity in biological samples. This assay kit offers a fluorometric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and cancer research.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect cathepsin B activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Cathepsin B Activity Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, serum, plasma, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cathepsin B in cellular processes, investigating its role in cancer progression, or screening potential inhibitors, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures cathepsin B activity based on the enzymatic cleavage of a fluorogenic substrate by cathepsin B. The substrate contains a fluorophore that is quenched in its intact state but becomes highly fluorescent upon cleavage by cathepsin B, allowing for the quantification of enzyme activity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCancer Research:\u003c\/strong\u003e Assess cathepsin B activity as a biomarker for tumor progression, metastasis, and therapeutic response in various cancer types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Biology:\u003c\/strong\u003e Study the role of cathepsin B in intracellular protein degradation, autophagy, and lysosomal function.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen compounds for their effects on cathepsin B activity and evaluate their potential as therapeutic agents for diseases associated with cathepsin dysregulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eFluorogenic Substrate\u003c\/li\u003e\n\u003cli\u003eCathepsin B Enzyme\u003c\/li\u003e\n\u003cli\u003eStop Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cathepsin B Activity Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Cathepsin B Activity Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing cathepsin B\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eFluorescence microplate reader capable of measuring fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Cathepsin B Enzyme provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Cathepsin B Enzyme to generate a standard curve. Typical concentrations may range from 0 to 1000 U\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003ePrepare control wells containing assay buffer only as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and Cathepsin B Standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Fluorogenic Substrate to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic cleavage of the substrate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eReaction Termination:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well to stop the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the fluorescence intensity of each well using a fluorescence microplate reader at the excitation and emission wavelengths provided by the kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the fluorescence values obtained from the Cathepsin B Standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the cathepsin B activity in your samples based on their respective fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in cathepsin B activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Cathepsin B Activity Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290035019,"sku":"AFG-BV-0190","price":392.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_e7372f51-13d5-46e6-82ea-6eb10537c200.png?v=1705258078"},{"product_id":"affiassay-cathepsin-d-activity-fluorometric-assay-kit","title":"AffiASSAY® Cathepsin D Activity Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cathepsin D Activity Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Cathepsin D Activity Fluorometric Assay Kit is designed to provide precise and reliable measurements of cathepsin D enzyme activity in biological samples. This assay kit offers a fluorometric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and cancer research.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect cathepsin D activity, allowing for accurate quantification in a wide range of samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorometric Detection:\u003c\/strong\u003e Utilizing a highly sensitive fluorometric assay, our kit ensures robust and reproducible results, minimizing the margin for error.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Cathepsin D Activity Fluorometric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, serum, plasma, and culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cathepsin D in cellular processes, investigating its role in cancer progression, or screening potential inhibitors, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures cathepsin D activity based on the enzymatic cleavage of a fluorogenic substrate by cathepsin D. The substrate contains a fluorophore that is quenched in its intact state but becomes highly fluorescent upon cleavage by cathepsin D, allowing for the quantification of enzyme activity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCancer Research:\u003c\/strong\u003e Assess cathepsin D activity as a biomarker for tumor progression, metastasis, and therapeutic response in various cancer types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Biology:\u003c\/strong\u003e Study the role of cathepsin D in intracellular protein degradation, autophagy, and lysosomal function.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen compounds for their effects on cathepsin D activity and evaluate their potential as therapeutic agents for diseases associated with cathepsin dysregulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAssay Buffer\u003c\/li\u003e\n\u003cli\u003eFluorogenic Substrate\u003c\/li\u003e\n\u003cli\u003eCathepsin D Enzyme\u003c\/li\u003e\n\u003cli\u003eStop Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Cathepsin D Activity Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Cathepsin D Activity Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing cathepsin D\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eFluorescence microplate reader capable of measuring fluorescence intensity at specific wavelengths\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReconstitute the Cathepsin D Enzyme provided in the kit according to the kit's instructions.\u003c\/li\u003e\n\u003cli\u003eCreate a series of standard dilutions using the reconstituted Cathepsin D Enzyme to generate a standard curve. Typical concentrations may range from 0 to 1000 U\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003ePrepare control wells containing assay buffer only as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and Cathepsin D Standards to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Fluorogenic Substrate to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic cleavage of the substrate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eReaction Termination:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well to stop the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eFluorescence Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the fluorescence intensity of each well using a fluorescence microplate reader at the excitation and emission wavelengths provided by the kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the fluorescence values obtained from the Cathepsin D Standards.\u003c\/li\u003e\n\u003cli\u003eCalculate the cathepsin D activity in your samples based on their respective fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in cathepsin D activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Cathepsin D Activity Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290067787,"sku":"AFG-BV-0191","price":392.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_3bef7e99-51e6-4fad-8553-c833de199fda.png?v=1705258081"},{"product_id":"affiassay-choline-acetylcholine-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Choline\/Acetylcholine Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Choline\/Acetylcholine Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Choline\/Acetylcholine Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of choline and acetylcholine levels in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in neuroscience, pharmacology, and neurology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect choline and acetylcholine levels with high sensitivity, allowing for accurate quantification even in low concentration samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric \u0026amp; Fluorometric Detection:\u003c\/strong\u003e With the flexibility to choose between colorimetric or fluorometric detection methods, our kit provides options for different experimental setups and preferences.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Choline\/Acetylcholine Assay Kit can be used with various sample types, including brain tissue, serum, plasma, and cell lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying cholinergic neurotransmission, investigating the effects of drugs on acetylcholine levels, or exploring choline metabolism, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures choline and acetylcholine levels based on the enzymatic reactions involving choline and acetylcholine. Choline is oxidized to betaine, releasing hydrogen peroxide in the process, which reacts with a colorimetric or fluorometric probe to generate a signal proportional to choline concentration. Acetylcholine is hydrolyzed to choline and acetate by acetylcholinesterase, and the released choline is then detected using the same enzymatic reaction as for choline measurement.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNeuroscience:\u003c\/strong\u003e Study cholinergic neurotransmission and its role in cognitive function, learning, and memory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePharmacology:\u003c\/strong\u003e Assess the effects of drugs targeting cholinergic receptors or acetylcholinesterase inhibitors.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNeurology:\u003c\/strong\u003e Investigate choline and acetylcholine levels as potential biomarkers for neurological disorders such as Alzheimer's disease.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCholine\/Acetylcholine Assay Buffer\u003c\/li\u003e\n\u003cli\u003eCholine\/Acetylcholine Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eCholine Probe\u003c\/li\u003e\n\u003cli\u003eAcetylcholine Probe\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Choline\/Acetylcholine Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Choline\/Acetylcholine Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing choline and\/or acetylcholine\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence intensity\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of choline and\/or acetylcholine.\u003c\/li\u003e\n\u003cli\u003eDilute the Choline\/Acetylcholine Probe provided in the kit with the Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without choline or acetylcholine to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Choline\/Acetylcholine Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSignal Detection:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFor colorimetric detection: Measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection: Measure the fluorescence intensity using a microplate reader with excitation and emission wavelengths suitable for the probes provided in the kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the choline and\/or acetylcholine concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in choline and\/or acetylcholine levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Choline\/Acetylcholine Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290100555,"sku":"AFG-BV-0703","price":430.83,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_539e5320-d1ee-4acf-a7b0-79c6eaec5efc.png?v=1705258083"},{"product_id":"affiassay-citrate-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Citrate Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Citrate Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Citrate Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of citrate levels in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in biochemistry, physiology, and nutrition.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect citrate levels with high sensitivity, allowing for accurate quantification even in low concentration samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric \u0026amp; Fluorometric Detection:\u003c\/strong\u003e With the flexibility to choose between colorimetric or fluorometric detection methods, our kit provides options for different experimental setups and preferences.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Citrate Assay Kit can be used with various sample types, including serum, plasma, urine, tissue homogenates, and cell lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying citrate metabolism, investigating its role in metabolic diseases, or assessing dietary intake, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures citrate levels based on the enzymatic reactions involving citrate. Citrate is metabolized to produce a product that reacts with a colorimetric or fluorometric probe to generate a signal proportional to citrate concentration.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Research:\u003c\/strong\u003e Study citrate metabolism and its involvement in various metabolic pathways, including the citric acid cycle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNutrition Studies:\u003c\/strong\u003e Assess dietary intake of citrate and its correlation with health outcomes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMedical Diagnostics:\u003c\/strong\u003e Measure citrate levels as potential biomarkers for metabolic disorders such as diabetes or metabolic syndrome.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCitrate Assay Buffer\u003c\/li\u003e\n\u003cli\u003eCitrate Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eCitrate Probe\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Citrate Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Citrate Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing citrate\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence intensity\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of citrate.\u003c\/li\u003e\n\u003cli\u003eDilute the Citrate Probe provided in the kit with the Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without citrate to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Citrate Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSignal Detection:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFor colorimetric detection: Measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection: Measure the fluorescence intensity using a microplate reader with excitation and emission wavelengths suitable for the probe provided in the kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the citrate concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in citrate levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Citrate Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290133323,"sku":"AFG-BV-0223","price":566.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_0553c0bc-00dd-4e61-b9e3-a99a883901ce.png?v=1705258086"},{"product_id":"affiassay-creatinine-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Creatinine Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Creatinine Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAffiASSAY® Creatinine Colorimetric \u0026amp; Fluorometric Assay Kit is designed to provide precise and reliable measurements of creatinine levels in biological samples. This assay kit offers both colorimetric and fluorometric detection methods, making it a versatile tool for researchers in clinical diagnostics, nephrology, and physiology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit is optimized to detect creatinine levels with high sensitivity, allowing for accurate quantification even in low concentration samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric \u0026amp; Fluorometric Detection:\u003c\/strong\u003e With the flexibility to choose between colorimetric or fluorometric detection methods, our kit provides options for different experimental setups and preferences.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Creatinine Assay Kit can be used with various sample types, including serum, plasma, urine, and cell culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e Whether you're studying renal function, assessing kidney health, or investigating drug-induced nephrotoxicity, our kit provides the versatility you need.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures creatinine levels based on the enzymatic reaction involving creatinine. Creatinine is converted to a product that reacts with a colorimetric or fluorometric probe to generate a signal proportional to creatinine concentration.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Diagnostics:\u003c\/strong\u003e Measure creatinine levels as a marker of renal function and kidney health in patient samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNephrology:\u003c\/strong\u003e Study renal physiology and pathophysiology, including acute kidney injury and chronic kidney disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Toxicology:\u003c\/strong\u003e Assess drug-induced nephrotoxicity by monitoring changes in creatinine levels.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCreatinine Assay Buffer\u003c\/li\u003e\n\u003cli\u003eCreatinine Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eCreatinine Probe\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Creatinine Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Creatinine Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing creatinine\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence intensity\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of creatinine.\u003c\/li\u003e\n\u003cli\u003eDilute the Creatinine Probe provided in the kit with the Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without creatinine to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of Creatinine Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSignal Detection:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFor colorimetric detection: Measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection: Measure the fluorescence intensity using a microplate reader with excitation and emission wavelengths suitable for the probe provided in the kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the creatinine concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eNote any variations or trends in creatinine levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Creatinine Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290198859,"sku":"AFG-BV-0233","price":302.58,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_c3051824-3f32-48bb-b8cb-1b55a8ec03eb.png?v=1705258088"},{"product_id":"affiassay-crystal-violet-cell-cytotoxicity-colorimetric-assay-kit","title":"AffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit provides a precise and reliable method for assessing cell viability and cytotoxicity in various biological samples. This assay kit utilizes the crystal violet dye staining method, offering a simple, efficient, and cost-effective solution for researchers in cell biology, drug discovery, and toxicology.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit detects subtle changes in cell viability, allowing for accurate assessment of cytotoxic effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing the crystal violet dye staining method, our kit provides a straightforward and easy-to-read colorimetric readout.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Crystal Violet Assay Kit is compatible with various cell types, including adherent and suspension cells, making it suitable for a wide range of experimental setups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit offers an affordable solution for assessing cell viability and cytotoxicity, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures cell viability and cytotoxicity based on the ability of viable cells to adhere to the culture plate and retain crystal violet dye. Dead or dying cells lose their ability to adhere and are washed away during the assay process, resulting in a decrease in the staining intensity.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen compounds for cytotoxic effects and assess their impact on cell viability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eToxicology Studies:\u003c\/strong\u003e Evaluate the cytotoxicity of environmental toxins, chemicals, or pharmaceutical agents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Biology:\u003c\/strong\u003e Study cell proliferation, growth inhibition, and cell death mechanisms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCrystal Violet Staining Solution\u003c\/li\u003e\n\u003cli\u003eFixation Solution\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eCell culture plates with adherent cells\u003c\/li\u003e\n\u003cli\u003eCell culture medium\u003c\/li\u003e\n\u003cli\u003eCell culture incubator\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eCell Seeding:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSeed cells in a suitable culture plate and incubate until they reach the desired confluence.\u003c\/li\u003e\n\u003cli\u003eEnsure that the cells are in the exponential growth phase for optimal results.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eRemove the culture medium from the cells and wash them gently with phosphate-buffered saline (PBS).\u003c\/li\u003e\n\u003cli\u003eAdd the Crystal Violet Staining Solution to each well and incubate for a specified time period.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eStaining:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eRemove the staining solution and wash the cells with PBS to remove excess dye.\u003c\/li\u003e\n\u003cli\u003eAdd the Fixation Solution to each well to fix the cells in place.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter fixation, solubilize the crystal violet dye with an appropriate solvent.\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance of each well at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the percentage of cell viability or cytotoxicity based on the absorbance values obtained from treated and untreated samples.\u003c\/li\u003e\n\u003cli\u003eCompare the results between different experimental conditions to assess the cytotoxic effects.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any changes in cell viability or cytotoxicity induced by experimental treatments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Crystal Violet Cell Cytotoxicity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290264395,"sku":"AFG-BV-0234","price":225.63,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_d69faa83-8a39-4b46-878e-89bc37daf245.png?v=1705258091"},{"product_id":"affiassay-d-lactate-colorimetric-assay-kit","title":"AffiASSAY® D-Lactate Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® D-Lactate Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® D-Lactate Colorimetric Assay Kit provides a precise and reliable method for measuring D-lactate levels in various biological samples. This assay kit offers a simple and efficient solution for researchers in fields such as clinical diagnostics, microbiology, and nutrition.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit can detect low levels of D-lactate, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a colorimetric assay, our kit offers a simple and easy-to-read readout, suitable for both manual and automated analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® D-Lactate Assay Kit is compatible with various sample types, including serum, plasma, urine, tissue homogenates, and cell lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring D-lactate levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThis assay kit measures D-lactate levels based on the enzymatic conversion of D-lactate to generate a product that reacts with a colorimetric probe. The intensity of the color formed is proportional to the D-lactate concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClinical Diagnostics:\u003c\/strong\u003e Measure D-lactate levels in patient samples for the diagnosis and monitoring of lactate acidosis, bacterial infections, and other metabolic disorders.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMicrobiology:\u003c\/strong\u003e Assess D-lactate production by bacteria and other microorganisms in culture media or biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNutrition Studies:\u003c\/strong\u003e Evaluate D-lactate levels in food products and beverages to assess fermentation processes and food quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eD-Lactate Assay Buffer\u003c\/li\u003e\n\u003cli\u003eD-Lactate Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eD-Lactate Probe\u003c\/li\u003e\n\u003cli\u003e96-Well Assay Plate\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® D-Lactate Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® D-Lactate Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing D-lactate\u003c\/li\u003e\n\u003cli\u003e96-well microplate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of D-lactate.\u003c\/li\u003e\n\u003cli\u003eDilute the D-Lactate Probe provided in the kit with the Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without D-lactate to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 µL of D-Lactate Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance of each well at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the D-lactate concentrations in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in D-lactate levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® D-Lactate Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290592075,"sku":"AFG-BV-0251","price":375.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_513d4dc8-7cc5-4b68-890a-83863b5b45aa.png?v=1705258094"},{"product_id":"affiassay-elastin-assay-kit-colorimetric","title":"AffiASSAY® Elastin Assay Kit (Colorimetric)","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Elastin Assay Kit (Colorimetric)\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Elastin Assay Kit (Colorimetric) provides a precise and reliable method for quantifying elastin content in various biological samples. This assay kit is designed to meet the needs of researchers in fields such as cardiovascular biology, connective tissue research, and pulmonary studies.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit can detect low levels of elastin, allowing for accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColorimetric Detection:\u003c\/strong\u003e Utilizing a colorimetric assay, our kit offers a simple and easy-to-read readout, suitable for both manual and automated analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Elastin Assay Kit is compatible with various sample types, including tissues, cell lysates, and extracellular matrix extracts.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring elastin levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe colorimetric assay measures the elastin content based on the reaction of elastin with a dye reagent to form a colored complex. The intensity of the color formed is directly proportional to the amount of elastin present in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCardiovascular Research:\u003c\/strong\u003e Study the role of elastin in cardiovascular diseases, such as atherosclerosis and hypertension.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConnective Tissue Studies:\u003c\/strong\u003e Investigate the structural integrity and elasticity of connective tissues in health and disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePulmonary Research:\u003c\/strong\u003e Assess elastin content in lung tissues to understand the pathogenesis of pulmonary diseases, including emphysema and fibrosis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eElastin Assay Buffer\u003c\/li\u003e\n\u003cli\u003eElastin Assay Reagent\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eElastin Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Elastin Assay Kit (Colorimetric) Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Elastin Assay Kit (Colorimetric)\u003c\/li\u003e\n\u003cli\u003eBiological samples containing elastin\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of elastin standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Elastin Assay Reagent with Elastin Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without elastin to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd an equal volume of Elastin Assay Reagent to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the color to develop.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance of each well at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate a standard curve using the absorbance values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the elastin concentrations in your samples based on their respective absorbance readings and the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in elastin levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Elastin Assay Kit (Colorimetric) for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290624843,"sku":"AFG-BV-0265","price":456.48,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_35a5c062-78af-4b29-8ec5-6f877e7cf2bc.png?v=1705258096"},{"product_id":"affiassay-ethanol-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Ethanol Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Ethanol Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Ethanol Colorimetric \u0026amp; Fluorometric Assay Kit provides a precise and reliable method for measuring ethanol levels in various biological samples. This assay kit offers flexibility with options for both colorimetric and fluorometric detection, catering to the diverse needs of researchers in fields such as toxicology, pharmacology, and alcohol metabolism studies.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection:\u003c\/strong\u003e Our assay kit allows for ethanol measurement using both colorimetric and fluorometric methods, offering flexibility based on experimental requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of ethanol, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Ethanol Assay Kit is compatible with various sample types, including blood, serum, urine, saliva, and cell culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring ethanol levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures ethanol levels based on the enzymatic conversion of ethanol to acetaldehyde and hydrogen peroxide. The hydrogen peroxide generated reacts with a probe to produce a colorimetric or fluorometric signal, which is directly proportional to the ethanol concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eToxicology Studies:\u003c\/strong\u003e Assess ethanol levels in biological samples to study the effects of alcohol consumption and exposure on cellular and physiological processes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePharmacological Research:\u003c\/strong\u003e Evaluate ethanol metabolism and clearance rates to understand drug-alcohol interactions and pharmacokinetics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlcohol Metabolism Studies:\u003c\/strong\u003e Investigate ethanol metabolism pathways and enzymatic activities in tissues and cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eEthanol Assay Buffer\u003c\/li\u003e\n\u003cli\u003eEthanol Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eEthanol Probe\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eEthanol Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Ethanol Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Ethanol Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing ethanol\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance and fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of ethanol standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Ethanol Probe with Ethanol Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without ethanol to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd an equal volume of Ethanol Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric and Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance and fluorescence of each well using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor colorimetric detection, measure the absorbance at the appropriate wavelength.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection, measure the fluorescence intensity using appropriate excitation and emission wavelengths.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate standard curves using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the ethanol concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in ethanol levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Ethanol Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290690379,"sku":"AFG-BV-0269","price":392.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_b9512378-80c8-4c0e-bcf8-e9b0e2c0887f.png?v=1705258099"},{"product_id":"affiassay-free-fatty-acid-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Free Fatty Acid Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Free Fatty Acid Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Free Fatty Acid Colorimetric \u0026amp; Fluorometric Assay Kit offers a reliable method for the quantification of free fatty acids (FFAs) in various biological samples. This assay kit provides flexibility with options for both colorimetric and fluorometric detection, making it suitable for a wide range of research applications in lipid metabolism, metabolic diseases, and nutritional studies.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection:\u003c\/strong\u003e Our assay kit enables the measurement of FFAs using both colorimetric and fluorometric methods, offering versatility based on experimental needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The kit can detect low levels of FFAs, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Free Fatty Acid Assay Kit is compatible with various sample types, including plasma, serum, tissues, cell lysates, and culture media.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring FFAs, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures FFAs based on the enzymatic hydrolysis of fatty acids to produce a colorimetric or fluorometric signal. The signal intensity is directly proportional to the concentration of FFAs in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLipid Metabolism Research:\u003c\/strong\u003e Study the regulation of lipid metabolism and the role of FFAs in various physiological processes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolic Disease Studies:\u003c\/strong\u003e Investigate the association between FFA levels and metabolic disorders such as obesity, diabetes, and cardiovascular diseases.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNutritional Research:\u003c\/strong\u003e Assess the impact of dietary interventions and nutritional supplements on FFA metabolism and lipid profiles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFree Fatty Acid Assay Buffer\u003c\/li\u003e\n\u003cli\u003eFree Fatty Acid Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eFree Fatty Acid Probe\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eFree Fatty Acid Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Free Fatty Acid Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Free Fatty Acid Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing free fatty acids\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance and fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the free fatty acid standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Free Fatty Acid Probe with Free Fatty Acid Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without free fatty acids to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd an equal volume of Free Fatty Acid Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reactions to occur.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric and Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance and fluorescence of each well using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor colorimetric detection, measure the absorbance at the appropriate wavelength.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection, measure the fluorescence intensity using appropriate excitation and emission wavelengths.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate standard curves using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the free fatty acid concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in free fatty acid levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Free Fatty Acid Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290723147,"sku":"AFG-BV-0294","price":366.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_5836adb0-dbe2-4e04-aceb-1e9e802c898b.png?v=1705258101"},{"product_id":"affiassay-gapdh-colorimetric-activity-assay-kit","title":"AffiASSAY® GAPDH Colorimetric Activity Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® GAPDH Colorimetric Activity Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® GAPDH Colorimetric Activity Assay Kit provides a reliable method for the quantification of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity in various biological samples. This assay kit offers a colorimetric detection method, enabling researchers to study glycolysis, cellular metabolism, and GAPDH-associated pathways with precision and convenience.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of GAPDH activity, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® GAPDH Colorimetric Activity Assay Kit is compatible with various sample types, including cell lysates, tissues, and purified enzyme preparations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring GAPDH activity, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures GAPDH activity based on the enzymatic conversion of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (1,3-BPG), coupled with the reduction of NAD\u003csup\u003e+\u003c\/sup\u003e to NADH. The increase in absorbance at 450 nm is directly proportional to the GAPDH activity in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGlycolysis Studies:\u003c\/strong\u003e Investigate the role of GAPDH in the glycolytic pathway and energy metabolism.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular Metabolism Research:\u003c\/strong\u003e Study the regulation of GAPDH activity in response to metabolic changes and cellular signaling pathways.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen for compounds that modulate GAPDH activity as potential therapeutics for metabolic disorders and cancer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGAPDH Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGAPDH Substrate Mix\u003c\/li\u003e\n\u003cli\u003eGAPDH Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eNAD\u003csup\u003e+\u003c\/sup\u003e\n\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® GAPDH Colorimetric Activity Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® GAPDH Colorimetric Activity Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing GAPDH\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without GAPDH to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the GAPDH Assay Buffer by diluting as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the GAPDH Substrate Mix and GAPDH Enzyme Mix to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at 450 nm using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the GAPDH activity in your samples based on the absorbance readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in GAPDH activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® GAPDH Colorimetric Activity Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294290755915,"sku":"AFG-BV-0312","price":302.58,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_0927e0e2-40de-42e6-a531-6e5d83ceb459.png?v=1705258104"},{"product_id":"affiassay-glucose-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Glucose Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glucose Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glucose Colorimetric \u0026amp; Fluorometric Assay Kit offers a reliable method for the quantification of glucose in various biological samples. This versatile assay kit provides flexibility with options for both colorimetric and fluorometric detection, making it suitable for a wide range of research applications in metabolism, diabetes, and glucose homeostasis studies.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection:\u003c\/strong\u003e Our assay kit enables the measurement of glucose using both colorimetric and fluorometric methods, offering versatility based on experimental needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The kit can detect low levels of glucose, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Glucose Assay Kit is compatible with various sample types, including serum, plasma, urine, cell lysates, and culture media.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring glucose levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures glucose based on the enzymatic oxidation of glucose to generate a colorimetric or fluorometric signal. The signal intensity is directly proportional to the glucose concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolism Studies:\u003c\/strong\u003e Investigate glucose metabolism and its role in energy production and storage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDiabetes Research:\u003c\/strong\u003e Study glucose homeostasis, insulin resistance, and diabetes-related complications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNutritional Studies:\u003c\/strong\u003e Assess the impact of dietary interventions and nutritional supplements on blood glucose levels and metabolic health.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGlucose Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGlucose Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eGlucose Probe\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eGlucose Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glucose Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glucose Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing glucose\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance and fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the glucose standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Glucose Probe with Glucose Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without glucose to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the Glucose Assay Buffer by diluting as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the Glucose Enzyme Mix and Glucose Probe to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric and Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance and fluorescence of each well using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eFor colorimetric detection, measure the absorbance at the appropriate wavelength.\u003c\/li\u003e\n\u003cli\u003eFor fluorometric detection, measure the fluorescence intensity using appropriate excitation and emission wavelengths.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate standard curves using the absorbance or fluorescence values obtained from the standard solutions.\u003c\/li\u003e\n\u003cli\u003eCalculate the glucose concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in glucose levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glucose Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294291968331,"sku":"AFG-BV-0324","price":294.03,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_6154684b-d5af-482b-b35e-2f0e3503fd1d.png?v=1705258106"},{"product_id":"affiassay-glutamate-colorimetric-assay-kit","title":"AffiASSAY® Glutamate Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutamate Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glutamate Colorimetric Assay Kit provides a reliable method for the quantification of glutamate in various biological samples. This assay kit offers a colorimetric detection method, enabling researchers to study glutamate metabolism, neurotransmission, and neurodegenerative disorders with precision and convenience.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of glutamate, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Glutamate Colorimetric Assay Kit is compatible with various sample types, including brain tissue homogenates, cerebrospinal fluid, and cell lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring glutamate levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures glutamate based on the enzymatic oxidation of glutamate by glutamate dehydrogenase (GLDH) to generate a colorimetric signal. The signal intensity is directly proportional to the glutamate concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNeuroscience Research:\u003c\/strong\u003e Investigate glutamate signaling, excitotoxicity, and synaptic transmission in neurological diseases such as Alzheimer's disease and Parkinson's disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolism Studies:\u003c\/strong\u003e Study the role of glutamate in amino acid metabolism, energy production, and neurotransmitter synthesis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen for compounds that modulate glutamate levels or activity as potential therapeutics for neurological disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGlutamate Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGlutamate Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eGlutamate Probe\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eGlutamate Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutamate Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glutamate Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing glutamate\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the glutamate standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Glutamate Probe with Glutamate Assay Buffer to prepare the working solution.\u003c\/li\u003e\n\u003cli\u003eAdd the working solution to each standard solution to initiate the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without glutamate to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the Glutamate Assay Buffer by diluting as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the Glutamate Enzyme Mix and Glutamate Probe to each well to initiate the reaction.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at 450 nm using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the glutamate concentrations in your samples based on the absorbance readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in glutamate levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glutamate Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292001099,"sku":"AFG-BV-0339","price":375.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_8121838a-47d5-4112-8554-905c45bb2b52.png?v=1705258109"},{"product_id":"affiassay-glutamine-colorimetric-assay-kit","title":"AffiASSAY® Glutamine Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutamine Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glutamine Colorimetric Assay Kit offers a reliable method for the quantification of glutamine in various biological samples. This assay kit provides a colorimetric detection method, enabling researchers to study glutamine metabolism, amino acid metabolism, and cellular signaling pathways with precision and convenience.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of glutamine, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Glutamine Colorimetric Assay Kit is compatible with various sample types, including cell lysates, tissue homogenates, and culture media.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring glutamine levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures glutamine based on the enzymatic conversion of glutamine to glutamate by glutaminase, coupled with the oxidation of NADH to NAD\u003csup\u003e+\u003c\/sup\u003e. The decrease in absorbance at 450 nm is directly proportional to the glutamine concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolism Studies:\u003c\/strong\u003e Investigate glutamine metabolism, including its role in amino acid biosynthesis, protein synthesis, and nitrogen transport.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Signaling:\u003c\/strong\u003e Study the involvement of glutamine in cellular signaling pathways, including mTOR signaling and glutaminolysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCancer Research:\u003c\/strong\u003e Explore the role of glutamine metabolism in cancer cell proliferation, survival, and metastasis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGlutamine Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGlutaminase Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eNADH\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eGlutamine Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutamine Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glutamine Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing glutamine\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 450 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the glutamine standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Glutamine Assay Buffer and Glutaminase Enzyme Mix as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without glutamine to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Glutamine Assay Buffer, Glutaminase Enzyme Mix, and NADH.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at 450 nm using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the glutamine concentrations in your samples based on the absorbance readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in glutamine levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glutamine Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292033867,"sku":"AFG-BV-0342","price":405.18,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_3883d485-0765-4fc4-845f-8ec31fc1928c.png?v=1705258111"},{"product_id":"affiassay-glutathione-peroxidase-activity-colorimetric-assay-kit","title":"AffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit offers a reliable method for the quantification of glutathione peroxidase (GPx) activity in biological samples. This assay kit provides a colorimetric detection method, enabling researchers to study antioxidant enzyme activity, oxidative stress, and redox signaling with precision and convenience.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of GPx activity, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e The AffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit is compatible with various sample types, including cell lysates, tissue homogenates, and plasma.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring GPx activity, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures GPx activity based on the reduction of hydroperoxide by GPx, coupled with the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) by GPx. The decrease in absorbance at 340 nm is directly proportional to GPx activity in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntioxidant Research:\u003c\/strong\u003e Investigate the role of GPx in protecting cells from oxidative damage and maintaining cellular redox homeostasis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePathophysiology Studies:\u003c\/strong\u003e Study the involvement of GPx activity in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery:\u003c\/strong\u003e Screen for compounds that modulate GPx activity as potential therapeutics for oxidative stress-related diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGPx Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGPx Substrate\u003c\/li\u003e\n\u003cli\u003eGPx Enzyme\u003c\/li\u003e\n\u003cli\u003eGlutathione Reductase (GR)\u003c\/li\u003e\n\u003cli\u003eNADPH\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eGPx Standard\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing GPx\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance at 340 nm\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the GPx standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the GPx Substrate and GPx Enzyme as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without GPx to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the GPx Assay Buffer, GPx Substrate, GPx Enzyme, Glutathione Reductase (GR), and NADPH.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at 340 nm using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the GPx activity in your samples based on the absorbance readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in GPx activity among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glutathione Peroxidase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292066635,"sku":"AFG-BV-0347","price":366.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_7b165d1d-40a8-402c-b532-456b9bf3a4eb.png?v=1705258114"},{"product_id":"affiassay-glycogen-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Glycogen Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit provides a reliable method for the quantification of glycogen content in various biological samples. This assay kit offers both colorimetric and fluorometric detection methods, allowing researchers to study glycogen metabolism, glycogen storage disorders, and carbohydrate metabolism with precision and flexibility.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection Methods:\u003c\/strong\u003e The kit offers both colorimetric and fluorometric assays, providing flexibility in detecting glycogen content based on experimental requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of glycogen, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including tissues, cells, and biological fluids, the AffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit offers versatility in glycogen analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring glycogen content, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures glycogen content based on the hydrolysis of glycogen to glucose by glycogenase, followed by the enzymatic oxidation of glucose to generate a colorimetric or fluorometric signal. The signal intensity is directly proportional to the glycogen concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolism Studies:\u003c\/strong\u003e Investigate glycogen metabolism in various tissues and cell types under different physiological and pathological conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisease Research:\u003c\/strong\u003e Study glycogen storage disorders and their underlying mechanisms using reliable glycogen quantification methods.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen for compounds that modulate glycogen metabolism as potential therapeutics for metabolic disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGlycogen Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGlycogenase Enzyme\u003c\/li\u003e\n\u003cli\u003eGlucose Assay Reagent\u003c\/li\u003e\n\u003cli\u003eGlucose Standard\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing glycogen\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the glucose standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Glycogen Assay Buffer and Glycogenase Enzyme as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without glycogen to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Glycogen Assay Buffer and Glycogenase Enzyme.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric or Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at the appropriate wavelength or fluorescence using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the glycogen content in your samples based on the absorbance or fluorescence readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in glycogen content among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glycogen Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292099403,"sku":"AFG-BV-0356","price":426.55,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_e579e4f2-c66e-44c5-a25c-06db5d2129ea.png?v=1705258117"},{"product_id":"affiassay-glycogen-colorimetric-assay-kit-v2","title":"AffiASSAY® Glycogen Colorimetric Assay Kit V2","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glycogen Colorimetric Assay Kit V2\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Glycogen Colorimetric Assay Kit V2 provides a reliable method for the quantification of glycogen content in various biological samples. This assay kit offers a colorimetric detection method, allowing researchers to study glycogen metabolism, glycogen storage disorders, and carbohydrate metabolism with precision and convenience.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of glycogen, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including tissues, cells, and biological fluids, the AffiASSAY® Glycogen Colorimetric Assay Kit V2 offers versatility in glycogen analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring glycogen content, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures glycogen content based on the hydrolysis of glycogen to glucose by glycogenase, followed by the enzymatic oxidation of glucose to generate a colorimetric signal. The signal intensity is directly proportional to the glycogen concentration in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetabolism Studies:\u003c\/strong\u003e Investigate glycogen metabolism in various tissues and cell types under different physiological and pathological conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisease Research:\u003c\/strong\u003e Study glycogen storage disorders and their underlying mechanisms using reliable glycogen quantification methods.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Screening:\u003c\/strong\u003e Screen for compounds that modulate glycogen metabolism as potential therapeutics for metabolic disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGlycogen Assay Buffer\u003c\/li\u003e\n\u003cli\u003eGlycogenase Enzyme\u003c\/li\u003e\n\u003cli\u003eGlucose Assay Reagent\u003c\/li\u003e\n\u003cli\u003eGlucose Standard\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Glycogen Colorimetric Assay Kit V2 Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Glycogen Colorimetric Assay Kit V2\u003c\/li\u003e\n\u003cli\u003eBiological samples containing glycogen\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the glucose standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Glycogen Assay Buffer and Glycogenase Enzyme as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without glycogen to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Glycogen Assay Buffer and Glycogenase Enzyme.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the glycogen content in your samples based on the absorbance readings and standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in glycogen content among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Glycogen Colorimetric Assay Kit V2 for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292132171,"sku":"AFG-BV-0701","price":328.23,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_ee516345-c652-496c-9405-32e53eccd9d4.png?v=1705258119"},{"product_id":"affiassay-hdl-ldl-cholesterol-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit provides a comprehensive solution for the quantification of both HDL (High-Density Lipoprotein) and LDL (Low-Density Lipoprotein) cholesterol levels in biological samples. This versatile assay kit offers both colorimetric and fluorometric detection methods, allowing researchers to assess lipid metabolism and investigate cardiovascular health with precision and flexibility.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection Methods:\u003c\/strong\u003e The kit offers both colorimetric and fluorometric assays, providing flexibility in detecting HDL and LDL cholesterol levels based on experimental requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of HDL and LDL cholesterol, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including serum, plasma, and tissue homogenates, the AffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit offers versatility in lipid metabolism studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring HDL and LDL cholesterol levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures HDL and LDL cholesterol levels based on the enzymatic reactions involving cholesterol esterase, cholesterol oxidase, and peroxidase. The reactions result in the formation of a colored product, which can be quantified using a microplate reader. The intensity of the color is directly proportional to the concentration of HDL and LDL cholesterol in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCardiovascular Research:\u003c\/strong\u003e Investigate the role of HDL and LDL cholesterol in cardiovascular health and disease.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLipid Metabolism Studies:\u003c\/strong\u003e Assess lipid metabolism and its regulation in various physiological and pathological conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Development:\u003c\/strong\u003e Screen for compounds that modulate HDL and LDL cholesterol levels as potential therapeutics for cardiovascular disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCholesterol Assay Buffer\u003c\/li\u003e\n\u003cli\u003eCholesterol Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eCholesterol Developer Solution\u003c\/li\u003e\n\u003cli\u003eCholesterol Standard\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing HDL and LDL cholesterol\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of the cholesterol standard provided in the kit.\u003c\/li\u003e\n\u003cli\u003eDilute the Cholesterol Assay Buffer, Cholesterol Enzyme Mix, and Cholesterol Developer Solution as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without cholesterol to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Cholesterol Assay Buffer, Cholesterol Enzyme Mix, and Cholesterol Developer Solution.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric or Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance or fluorescence at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the HDL and LDL cholesterol levels in your samples based on the absorbance or fluorescence readings and the standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in HDL and LDL cholesterol levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® HDL-LDL Cholesterol Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292164939,"sku":"AFG-BV-0699","price":379.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_40d2fa2f-101c-4bcb-bee0-58595a923518.png?v=1705258122"},{"product_id":"affiassay-hemin-colorimetric-assay-kit","title":"AffiASSAY® Hemin Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan data-mce-fragment=\"1\" style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hemin Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Hemin Colorimetric Assay Kit is a cutting-edge solution designed to streamline and simplify the quantification of hemin in your research samples. Hemin, also known as haematin, is a vital component in various biological processes and is frequently used in the study of heme-containing proteins, such as hemoglobin and cytochromes. With our user-friendly kit, you can achieve accurate and reproducible results with ease, saving you time and effort in the laboratory.\u003c\/p\u003e\n\u003ch3\u003eKey Features:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Our assay kit boasts an exceptional sensitivity, enabling the detection of even trace amounts of hemin in your samples. This makes it suitable for a wide range of applications, from basic research to clinical studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple Colorimetric Assay:\u003c\/strong\u003e The kit employs a straightforward colorimetric assay method, making it accessible to both novice and experienced researchers. The color change is directly proportional to the concentration of hemin in your sample, facilitating precise quantification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Efficient:\u003c\/strong\u003e Say goodbye to lengthy procedures and complex protocols. The AffiASSAY® Hemin Colorimetric Assay Kit offers rapid results, allowing you to increase your workflow efficiency and productivity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Dynamic Range:\u003c\/strong\u003e This assay kit provides a wide dynamic range, accommodating various sample concentrations without the need for dilution or concentration steps. This versatility ensures you can work with diverse sample types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReliable and Reproducible:\u003c\/strong\u003e With stringent quality control standards and optimized reagents, you can trust in the accuracy and reproducibility of your hemin measurements, ensuring consistent results from experiment to experiment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetailed Protocol:\u003c\/strong\u003e Included in the kit is a comprehensive protocol that guides you through the assay step-by-step, ensuring that you can perform the assay with confidence and achieve reliable results.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e The AffiASSAY® Hemin Colorimetric Assay Kit is suitable for a variety of applications, including heme-containing protein research, clinical diagnostics, drug development, and more.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHow It Works:\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your samples and standards according to the provided protocol.\u003c\/li\u003e\n\u003cli\u003eAdd the assay buffer and samples\/standards to the assay plate.\u003c\/li\u003e\n\u003cli\u003eAdd the developer solution to each well and incubate.\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eCalculate the hemin concentration in your samples based on the standard curve generated from the standards.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications:\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eResearch on heme-containing proteins\u003c\/li\u003e\n\u003cli\u003eDrug discovery and development\u003c\/li\u003e\n\u003cli\u003eClinical diagnostics\u003c\/li\u003e\n\u003cli\u003eBiochemical studies\u003c\/li\u003e\n\u003cli\u003eHematology research\u003c\/li\u003e\n\u003cli\u003eBiomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eOrder Your AffiASSAY® Hemin Colorimetric Assay Kit Today:\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eUnlock the power of precise hemin quantification in your research with the AffiASSAY® Hemin Colorimetric Assay Kit. Whether you are working in academia, industry, or clinical settings, this kit is your key to achieving accurate and reproducible results. Order now and experience the simplicity and efficiency of our cutting-edge solution. Elevate your research with AffiASSAY® today!\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hemin Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eAssay Procedure:\u003c\/h3\u003e\n\u003ch4\u003eSample Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your samples, ensuring they are properly homogenized if necessary. The concentration of hemin in your samples should be within the dynamic range of the assay.\u003c\/li\u003e\n\u003cli\u003eIf your samples are in a solid form, you may need to extract hemin according to your specific application. Consult relevant literature or resources for sample preparation guidance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a set of hemin standards with known concentrations. Typically, you would use a series of standard solutions with concentrations ranging from low to high.\u003c\/li\u003e\n\u003cli\u003eIt is recommended to prepare standard dilutions using the provided Assay Buffer or a suitable diluent as per the kit instructions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the wells of the assay plate(s) accordingly. Designate some wells for your standards, and others for your samples and controls.\u003c\/li\u003e\n\u003cli\u003eIt's advisable to include blank wells containing only the Assay Buffer to account for any background absorbance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50 μL of each standard solution, sample, and control into the respective wells of the assay plate.\u003c\/li\u003e\n\u003cli\u003eAdd 50 μL of the Assay Buffer to each well.\u003c\/li\u003e\n\u003cli\u003eMix the contents of each well gently by pipetting up and down or by tapping the plate to ensure proper mixing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAdd Developer Solution:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eQuickly add 100 μL of the Developer Solution to each well, ensuring thorough mixing.\u003c\/li\u003e\n\u003cli\u003eIncubate the plate at a specified temperature (as per the kit manual) for a recommended duration to allow the color to develop. This incubation time may vary, but it is typically around 10-20 minutes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAbsorbance Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance of the contents of each well at the appropriate wavelength using a microplate reader. The specific wavelength for measuring hemin absorbance should be provided in the kit manual.\u003c\/li\u003e\n\u003cli\u003eRecord the absorbance values for all the standards, samples, and controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUse the absorbance values obtained from the standard solutions to generate a standard curve.\u003c\/li\u003e\n\u003cli\u003eCalculate the hemin concentration in your samples by comparing their absorbance values to the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults and Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eYour results will indicate the concentration of hemin in your samples.\u003c\/li\u003e\n\u003cli\u003eEnsure you follow any specific data analysis procedures provided in the kit manual.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eQuality Control:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAlways perform appropriate quality control checks as recommended in the kit manual to ensure the accuracy and reproducibility of your results.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003eReporting:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eReport your findings, including the concentration of hemin in your samples, any statistical analyses performed, and any other relevant information, as required for your research or application.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eRemember to adhere to the specific instructions provided in the AffiASSAY® Hemin Colorimetric Assay Kit manual to ensure the best results and accuracy in your hemin quantification experiments.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292197707,"sku":"AFG-BV-0704","price":590.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_d42cc24c-93b6-4546-a26b-c1d8bc5a7a1d.png?v=1705258125"},{"product_id":"affiassay-hydrogen-peroxide-colorimetric-fluorometric-assay-kit","title":"AffiASSAY® Hydrogen Peroxide Colorimetric \u0026 Fluorometric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit offers a reliable method for the quantification of hydrogen peroxide levels in various biological samples. This versatile assay kit provides both colorimetric and fluorometric detection methods, allowing researchers to study oxidative stress, enzymatic reactions, and cellular signaling pathways with precision and flexibility.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDual Detection Methods:\u003c\/strong\u003e The kit offers both colorimetric and fluorometric assays, providing flexibility in detecting hydrogen peroxide levels based on experimental requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of hydrogen peroxide, ensuring accurate quantification in biological samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including cell lysates, tissue homogenates, and biological fluids, the AffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit offers versatility in oxidative stress studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring hydrogen peroxide levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures hydrogen peroxide levels based on the enzymatic reaction involving horseradish peroxidase (HRP), which oxidizes the substrate to produce a colored or fluorescent product. The intensity of the color or fluorescence is directly proportional to the concentration of hydrogen peroxide in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eOxidative Stress Research:\u003c\/strong\u003e Investigate the role of hydrogen peroxide in oxidative stress-related diseases and cellular damage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzymatic Reaction Studies:\u003c\/strong\u003e Assess the activity of enzymes involved in hydrogen peroxide metabolism and signaling pathways.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Signaling Pathways:\u003c\/strong\u003e Study the involvement of hydrogen peroxide in cellular signaling events and redox regulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eHydrogen Peroxide Assay Buffer\u003c\/li\u003e\n\u003cli\u003eHydrogen Peroxide Substrate\u003c\/li\u003e\n\u003cli\u003eHydrogen Peroxide Enzyme Mix\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing hydrogen peroxide\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance or fluorescence\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of hydrogen peroxide.\u003c\/li\u003e\n\u003cli\u003eDilute the Hydrogen Peroxide Assay Buffer, Hydrogen Peroxide Substrate, and Hydrogen Peroxide Enzyme Mix as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without hydrogen peroxide to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Hydrogen Peroxide Assay Buffer, Hydrogen Peroxide Substrate, and Hydrogen Peroxide Enzyme Mix.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric or Fluorometric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance or fluorescence at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the hydrogen peroxide levels in your samples based on the absorbance or fluorescence readings and the standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in hydrogen peroxide levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Hydrogen Peroxide Colorimetric \u0026amp; Fluorometric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"200 Assays","offer_id":47294292230475,"sku":"AFG-BV-0395","price":272.65,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_4e502fd5-5c5f-404d-a1c2-ddcf2da681be.png?v=1705258127"},{"product_id":"affiassay-hydroxyproline-colorimetric-assay-kit","title":"AffiASSAY® Hydroxyproline Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hydroxyproline Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Hydroxyproline Colorimetric Assay Kit provides a reliable method for the quantification of hydroxyproline levels in various biological samples. Hydroxyproline is a major component of collagen, and its measurement is crucial for studying collagen turnover, extracellular matrix remodeling, and tissue fibrosis. This assay kit offers a sensitive colorimetric detection method, allowing researchers to accurately assess hydroxyproline content with ease.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of hydroxyproline, ensuring accurate quantification in biological samples with minimal interference.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including tissues, cell lysates, and biological fluids, the AffiASSAY® Hydroxyproline Colorimetric Assay Kit offers versatility in collagen-related research.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring hydroxyproline levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures hydroxyproline levels based on the chloramine-T oxidation method. Hydroxyproline reacts with chloramine-T to form an intermediate, which reacts with p-dimethylaminobenzaldehyde (DMAB) to produce a colored compound. The intensity of the color is directly proportional to the concentration of hydroxyproline in the sample.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCollagen Turnover:\u003c\/strong\u003e Study collagen synthesis and degradation processes in physiological and pathological conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExtracellular Matrix Remodeling:\u003c\/strong\u003e Assess tissue remodeling events associated with wound healing, fibrosis, and tissue repair.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFibrosis Research:\u003c\/strong\u003e Investigate the role of hydroxyproline in tissue fibrosis and explore potential therapeutic interventions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eHydroxyproline Assay Buffer\u003c\/li\u003e\n\u003cli\u003eHydroxyproline Chloramine-T Reagent\u003c\/li\u003e\n\u003cli\u003eHydroxyproline DMAB Reagent\u003c\/li\u003e\n\u003cli\u003eHydroxyproline Standard\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Hydroxyproline Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Hydroxyproline Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing hydroxyproline\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of hydroxyproline.\u003c\/li\u003e\n\u003cli\u003eDilute the Hydroxyproline Assay Buffer, Hydroxyproline Chloramine-T Reagent, and Hydroxyproline DMAB Reagent as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without hydroxyproline to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Hydroxyproline Assay Buffer, Hydroxyproline Chloramine-T Reagent, and Hydroxyproline DMAB Reagent.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the color development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the hydroxyproline levels in your samples based on the absorbance readings and the standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in hydroxyproline levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Hydroxyproline Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292263243,"sku":"AFG-BV-0398","price":409.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_a79ad7eb-87fc-4a18-abf3-8b5cbbff032d.png?v=1705258130"},{"product_id":"affiassay-iron-colorimetric-assay-kit","title":"AffiASSAY® Iron Colorimetric Assay Kit","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan style=\"color: #7ad1c9;\" data-mce-fragment=\"1\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Iron Colorimetric Assay Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe AffiASSAY® Iron Colorimetric Assay Kit offers a reliable method for the quantification of iron levels in various biological samples. Iron is an essential mineral involved in various physiological processes, and its measurement is crucial for studying iron metabolism, iron deficiency, and iron overload conditions. This assay kit provides a sensitive colorimetric detection method, allowing researchers to accurately assess iron content with ease.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e The assay kit can detect low levels of iron, ensuring accurate quantification in biological samples with minimal interference.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Application:\u003c\/strong\u003e Compatible with various sample types, including serum, plasma, tissue homogenates, and cell lysates, the AffiASSAY® Iron Colorimetric Assay Kit offers versatility in iron-related research.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuick and Easy:\u003c\/strong\u003e With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Our kit provides an affordable solution for measuring iron levels, reducing experimental costs without compromising data quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAssay Principle:\u003c\/h3\u003e\n\u003cp\u003eThe assay kit measures iron levels based on the formation of a colored complex between iron ions and a chromogenic reagent. The intensity of the color is directly proportional to the concentration of iron in the sample, allowing for precise quantification.\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIron Metabolism:\u003c\/strong\u003e Study iron uptake, transport, and storage mechanisms in physiological and pathological conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAnemia Research:\u003c\/strong\u003e Assess iron deficiency and anemia prevalence in clinical and research settings.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIron Overload Conditions:\u003c\/strong\u003e Investigate the role of iron overload in diseases such as hemochromatosis and hemosiderosis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Contents:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIron Assay Buffer\u003c\/li\u003e\n\u003cli\u003eIron Chromogenic Reagent\u003c\/li\u003e\n\u003cli\u003eIron Reducing Agent\u003c\/li\u003e\n\u003cli\u003eIron Standard\u003c\/li\u003e\n\u003cli\u003eMicroplate (96 wells)\u003c\/li\u003e\n\u003cli\u003eDetailed Protocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhy Choose AffiASSAY®?\u003c\/h3\u003e\n\u003cp\u003eOur commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #7ad1c9;\" data-mce-style=\"color: #7ad1c9;\"\u003eAffiASSAY® Iron Colorimetric Assay Kit Protocol:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #b4648d;\"\u003e\u003cstrong\u003e[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Needed:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffiASSAY® Iron Colorimetric Assay Kit\u003c\/li\u003e\n\u003cli\u003eBiological samples containing iron\u003c\/li\u003e\n\u003cli\u003eMicroplate reader capable of measuring absorbance\u003c\/li\u003e\n\u003cli\u003ePipettes and tips\u003c\/li\u003e\n\u003cli\u003eMicrocentrifuge tubes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol:\u003c\/h3\u003e\n\u003ch4\u003eStandard Curve Preparation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare a series of standard solutions containing known concentrations of iron.\u003c\/li\u003e\n\u003cli\u003eDilute the Iron Assay Buffer, Iron Chromogenic Reagent, and Iron Reducing Agent as instructed in the kit manual.\u003c\/li\u003e\n\u003cli\u003eAdd the standard solutions to each well of the microplate to generate the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Plate Setup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLabel the microplate wells according to your experimental design, including wells for your samples, standards, and controls.\u003c\/li\u003e\n\u003cli\u003eIf using controls, prepare control samples without iron to serve as background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eSample and Standard Addition:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.\u003c\/li\u003e\n\u003cli\u003eEnsure that the final reaction volume in each well is consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eAssay Reaction:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare the reaction mixture by combining the Iron Assay Buffer, Iron Chromogenic Reagent, and Iron Reducing Agent.\u003c\/li\u003e\n\u003cli\u003eAdd the reaction mixture to each well of the microplate containing the samples and standards.\u003c\/li\u003e\n\u003cli\u003eIncubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the color development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eColorimetric Measurement:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAfter the incubation period, measure the absorbance at the appropriate wavelength using a microplate reader.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Analysis:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCalculate the iron levels in your samples based on the absorbance readings and the standard curve generated from the standard solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eResults Interpretation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eInterpret and analyze your results according to your research objectives.\u003c\/li\u003e\n\u003cli\u003eObserve any variations or trends in iron levels among your samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eData Presentation:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003ePrepare your data for presentation or publication, and include the appropriate statistical analysis if required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eCleanup:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDispose of all waste materials, including used assay components, according to your laboratory's safety protocols.\u003c\/li\u003e\n\u003cli\u003eAlways refer to the specific instructions provided with the AffiASSAY® Iron Colorimetric Assay Kit for any kit-specific details or variations in the protocol.\u003c\/li\u003e\n\u003cli\u003eAdditionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"100 Assays","offer_id":47294292296011,"sku":"AFG-BV-0409","price":328.23,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/affiassay-affigen_1500x_12fa61ab-db02-47c7-9bfe-19be3b21ae1e.png?v=1705258133"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/collections\/AffiASSAY.png?v=1722792823","url":"https:\/\/affigen.com\/nl-nl\/collections\/affiassay.oembed?page=3","provider":"AffiGEN","version":"1.0","type":"link"}