{"title":"siRNA In Vitro Transfection Reagents","description":"","products":[{"product_id":"affigen-transfect-tm-dna-in-vitro-4000","title":"AffiGEN® Transfect(TM) DNA \u0026 siRNA In Vitro 4000 Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch4\u003e\u003cspan style=\"color: #00d0c5;\"\u003eProduct Description\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit, harnessed from our innovative and proprietary lipid-conjugation technology, incorporating novel fluorinated cationic lipids, showcases substantial advantages over other lipid-based transfection reagents in the market. AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit represents the most potent yet gentle gene delivery solution, aptly suited for a diverse array of applications, including the transfection of plasmid DNA and\/or siRNA into most mammalian cell types. When compared to leading products in the market, AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit proves to be not only more cost-effective but consistently delivers superior transfection efficiency with reduced cytotoxicity.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #00d0c5;\"\u003eKit content\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e - AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit Reagent, 1.0 ml, sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).\u003cbr\u003e - AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5x), 8.0 mL, to prepare a 40 mL working solution (1x).\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #28c3bb;\"\u003eFeatures\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e - Versatile: Supports DNA transfection, siRNA transfection, and DNA\/siRNA co-transfection.\u003cbr\u003e - Powerful: Achieves exceptional efficiency with adherent cells.\u003cbr\u003e - Remarkably low toxicity: Enhances cell viability post-transfection.\u003cbr\u003e - Economical: Enables more transfections with less reagent.\u003cbr\u003e - User-friendly: Conducted as a one-tube reaction and compatible with serum\/antibiotics.\u003cbr\u003e - Ideal for high-throughput applications.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eStore AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit Reagent at 4°C and AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5x) at room temperature (RT). When stored correctly, the product maintains stability for 12 months or longer.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eDNA In Vitro Transfection Reagents Specifications\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ccolgroup\u003e \u003ccol width=\"30%\"\u003e \u003ccol width=\"10%\"\u003e \u003ccol width=\"15%\"\u003e \u003ccol width=\"15%\"\u003e \u003ccol width=\"10%\"\u003e \u003ccol width=\"10%\"\u003e \u003ccol width=\"10%\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eType\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eDelivery Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eToxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSerum Tolerance\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-1000-mammalian-cell\" title=\"AffiGEN® Transfect(TM) 1000 - Mammalian Cell\"\u003eAffiGEN® Transfect™ 1000 - Mammalian Cell\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eCalcium phosphate\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePrecipitation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v1\" title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 V1\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 V1\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA, siRNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer complex\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v2\" title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 V2\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 V2\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA, siRNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer complex\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigher\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMedium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-plus\" title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 Plus\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer complex\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigher\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMedium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-3000\" title=\"AffiGEN® Transfect(TM) DNA In Vitro 3000\"\u003eAffiGEN® Transfect™ DNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLiposomal\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLiposome-mediated\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\" title=\"AffiGEN® Transfect(TM) DNA \u0026amp; siRNA In Vitro 4000 Kit\"\u003eAffiGEN® Transfect™ DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA, siRNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLiposomal\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLiposome-mediated\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVery low\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-5000\" title=\"AffiGEN® Transfect(TM) DNA In Vitro 5000\"\u003eAffiGEN® Transfect™ DNA In Vitro 5000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDNA, siRNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePolymer complex\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eDNA In Vitro Transfection Reagents Application Guide\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ccolgroup\u003e \u003ccol width=\"25%\"\u003e \u003ccol width=\"20%\"\u003e \u003ccol width=\"15%\"\u003e \u003ccol width=\"10%\"\u003e \u003ccol width=\"15%\"\u003e \u003ccol width=\"15%\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eCell Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eCell lines\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSuitable for\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) 1000 - Mammalian Cell\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-1000-mammalian-cell\"\u003eAffiGEN® Transfect™ 1000 - Mammalian Cell\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein production, stable cell line generation, viral vector production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAdherent, some suspension\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHEK293, HepG2, CHO\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLarge-scale transfection, cost-effective\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eLower calcium concentration than traditional methods\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 V1\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v1\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 V1\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eGene expression studies, protein production, virus production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMost, including sensitive\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSensitive cell lines, maximizing cell viability\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eMultiple formulations available\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 V2\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v2\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 V2\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eGene expression studies, protein production, virus production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMost, including sensitive\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBalancing efficiency and viability\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eReleased from DNA complex\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA In Vitro 2000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-plus\"\u003eAffiGEN® Transfect™ DNA In Vitro 2000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein production, viral vector production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMost, including sensitive\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLong DNA, high efficiency\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eProprietary polymer\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA In Vitro 3000\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-3000\"\u003eAffiGEN® Transfect™ DNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eViral vector production, antibody production, protein production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAdherent, suspension\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLentivirus production, high efficiency\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eEnhanced conjugate\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA \u0026amp; siRNA In Vitro 4000 Kit\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAffiGEN® Transfect™ DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein production, gene editing, virus production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMost, including sensitive\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLow toxicity, high efficiency\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eFluorinated cationic lipids\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca title=\"AffiGEN® Transfect(TM) DNA In Vitro 5000\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-5000\"\u003eAffiGEN® Transfect™ DNA In Vitro 5000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eGene expression studies, protein production, virus production\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMost, including sensitive\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eVarious\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eCommon cell lines, biodegradable\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eBiodegradable polycationic polymer\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Very Low Toxicity - DNA \u0026 siRNA Transfection - Best Transfection with Least Toxicity","offer_id":47107566240075,"sku":"AFG-SNG-TR-011","price":410.4,"currency_code":"USD","in_stock":true},{"title":"5 X 1 mL \/ Very Low Toxicity - DNA \u0026 siRNA Transfection - Best Transfection with Least Toxicity","offer_id":47107565748555,"sku":"AFG-SNG-TR-012","price":1574.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-11.png?v=1700314138"},{"product_id":"affigen-transfect-tm-dna-in-vitro-4000-buffer-5x","title":"AffiGEN® Transfect(TM) DNA \u0026 siRNA In Vitro 4000 Buffer (5X)","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5X)\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch4\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eProduct Description\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5X) has been specifically designed to complement the \u003ca title=\"AffiGEN® Transfect(TM) DNA \u0026amp; siRNA In Vitro 4000 Kit\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/a\u003e (Cat# \u003ca title=\"AFG-SNG-TR-11\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAFG-SNG-TR-11\u003c\/a\u003e \u0026amp; \u003ca title=\"AFG-SNG-TR-12\" href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAFG-SNG-TR-12\u003c\/a\u003e), ensuring maximal DNA transfection efficiency and optimal gene silencing outcomes. Prior to use, it is necessary to dilute the AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5X) by adding 4 parts of ddH2O. The resulting AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer working solution is employed to dilute DNA, siRNA, and the AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Reagent to form the most effective transfection complex.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3acbc3;\"\u003ePackage\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eEach vial of AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5X) contains 8.0 mL of the solution, which, when appropriately diluted, yields a 40 mL working solution (1X). This quantity is sufficient for approximately 600 transfections in a 24-well plate.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eStore AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Buffer (5X) at room temperature (RT). When stored under proper conditions, the product remains stable for 12 months or longer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eDNA In Vitro Transfection Reagents Specifications\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\" data-mce-selected=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"20%\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"10%\"\u003e\u003cstrong\u003eType\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Toxicity\"}' width=\"10%\"\u003e\u003cstrong\u003eToxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Efficiency\"}' width=\"10%\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Serum Tolerance\"}' width=\"10%\"\u003e\u003cstrong\u003eSerum Tolerance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Delivery Method\"}' width=\"10%\"\u003e\u003cstrong\u003eDelivery Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Transfection Type\"}' width=\"10%\"\u003e\u003cstrong\u003eTransfection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Package\"}' width=\"10%\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Shelf Life\"}' width=\"10%\"\u003eShelf Life\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Additional Notes\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-1000-mammalian-cell\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e 1000 - Mammalian Cell\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Low\"}'\u003eLow\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Calcium Phosphate Precipitation\"}'\u003eCalcium Phosphate Precipitation\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Non-liposomal\"}'\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"No serum required, simple protocol, can be cytotoxic in some cell lines\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v1\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 V1\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Low\"}'\u003eLow\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Liposomal\"}'\u003eLiposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Cationic Liposomal\"}'\u003eCationic Liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High efficiency in stem cells, low cytotoxicity\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v2\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 V2\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Liposomal\"}'\u003eLiposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Cationic Liposomal\"}'\u003eCationic Liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Efficient for virus production, compatible with wide range of media\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Liposomal\"}'\u003eLiposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Cationic Liposomal\"}'\u003eCationic Liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Improved efficiency and viability compared to AffiGEN® Transfect\u0026lt;sup\u0026gt;TM\u0026lt;\/sup\u0026gt; DNA In Vitro 2000 V2, reduced cytotoxicity\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Low\"}'\u003eLow\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Liposomal\"}'\u003eLiposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Cationic Liposomal\"}'\u003eCationic Liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High efficiency with serum\/antibiotics, compatible with wide range of media\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA \u0026amp; siRNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Low\"}'\u003eLow\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Moderate\"}'\u003eModerate\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Polymer-based\"}'\u003ePolymer-based\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"N\/A\"}'\u003eN\/A\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Biodegradable, compatible with wide range of media, low cytotoxicity\"}' width=\"348\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-5000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 5000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eDNA\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Low\"}'\u003eLow\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High\"}'\u003eHigh\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Liposomal\"}'\u003eLiposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Cationic Liposomal\"}'\u003eCationic Liposomal\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"1 mL, 5X 1 mL\"}'\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e2 years at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eDNA In Vitro Transfection Reagents Application Guide\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\" data-sheets-root=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"20%\" data-sheets-value='{\"1\":2,\"2\":\"Product\"}'\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" data-sheets-value='{\"1\":2,\"2\":\"Target Organisms\"}'\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" data-sheets-value='{\"1\":2,\"2\":\"Applications\"}'\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"176\" data-sheets-value='{\"1\":2,\"2\":\"Cell Types\"}'\u003e\u003cstrong\u003eCell Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\u003cstrong\u003eSuitable for\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" data-sheets-value='{\"1\":2,\"2\":\"Additional Notes\"}'\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-1000-mammalian-cell\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e 1000 - Mammalian Cell\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, protein production\"}'\u003eGene expression, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eVirus, antibody and protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"No serum required, simple protocol, can be cytotoxic in some cell lines\"}'\u003eNo serum required, simple protocol, can be cytotoxic in some cell lines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v1\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 V1\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Mammalian cells (HEK293, CHO, COS, etc.)\"}'\u003eMammalian cells (HEK293, CHO, COS, etc.)\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, virus production, antibody production\"}'\u003eGene expression, virus production, antibody production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"HEK293, CHO, COS, stem cells\"}'\u003eHEK293, CHO, COS, stem cells\u003c\/td\u003e\n\u003ctd\u003eSensitive Cells \u0026amp; maximum cell viability\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High efficiency in stem cells, low cytotoxicity\"}'\u003eHigh efficiency in stem cells, low cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-v2\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 V2\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, virus production, protein production\"}'\u003eGene expression, virus production, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eCommon cell types\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Efficient for virus production, compatible with wide range of media\"}'\u003eEfficient for virus production, compatible with wide range of media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-2000-plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, protein production\"}'\u003eGene expression, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eVery long DNAs\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Improved efficiency and viability compared to AffiGEN® Transfect\u0026lt;sup\u0026gt;TM\u0026lt;\/sup\u0026gt; DNA In Vitro 2000 V2, reduced cytotoxicity\"}'\u003eImproved efficiency and viability compared to AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 2000 V2, reduced cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, virus production, protein production\"}'\u003eGene expression, virus production, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eLentivirus, antibody \u0026amp; protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High efficiency with serum\/antibiotics, compatible with wide range of media\"}'\u003eHigh efficiency with serum\/antibiotics, compatible with wide range of media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA \u0026amp; siRNA In Vitro 4000 Kit\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, protein production\"}'\u003eGene expression, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eBest transfection with least toxicity\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Biodegradable, compatible with wide range of media, low cytotoxicity\"}'\u003eBiodegradable, compatible with wide range of media, low cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"2 years at 4°C\"}'\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfect-tm-dna-in-vitro-5000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e DNA In Vitro 5000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect mammalian cells\"}'\u003eWide range, including hard-to-transfect mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Gene expression, virus production, protein production\"}'\u003eGene expression, virus production, protein production\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\"}'\u003eWide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.)\u003c\/td\u003e\n\u003ctd\u003eCommon mammalian cells\u003c\/td\u003e\n\u003ctd data-sheets-value='{\"1\":2,\"2\":\"High in-vivo delivery efficiency, compatible with wide range of media\"}'\u003eHigh in-vivo delivery efficiency, compatible with wide range of media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47107615752523,"sku":"AFG-SNG-TR-015","price":102.75,"currency_code":"USD","in_stock":true},{"title":"4 mL","offer_id":47107600417099,"sku":"AFG-SNG-TR-016","price":111.0,"currency_code":"USD","in_stock":true},{"title":"8 mL","offer_id":47107593732427,"sku":"AFG-SNG-TR-017","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen-transfect-buffer.png?v=1700313793"},{"product_id":"affigen-transfecttm-sirna-in-vitro-1000","title":"AffiGEN® Transfect(TM) siRNA In Vitro 1000","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eIntroducing AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000, a revolutionary tool for siRNA delivery. This innovative reagent, inspired by the simulation of virus cell-penetrating peptides (CPPs), achieves exceptional results, boasting over 95% silencing efficiency when used with just 1 nM siRNA across a range of mammalian cells.\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000\u003c\/span\u003e emerged as the standout choice, validated for its remarkable ability to condense and transfect short nucleic acids, including siRNA, miRNA mimics, and DNA oligos (under 100 bp), into a wide spectrum of mammalian cells.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eKey Features:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUnprecedented silencing efficacy at just 1.0 nM siRNA concentration\u003c\/li\u003e\n\u003cli\u003eAchieves over 95% gene silencing across diverse cell types\u003c\/li\u003e\n\u003cli\u003eStreamlined one-tube reaction with a straightforward standard protocol\u003c\/li\u003e\n\u003cli\u003eCompatible with serum and antibiotics for added versatility\u003c\/li\u003e\n\u003cli\u003eEasily adaptable to High Throughput Screening (HTS) applications\u003c\/li\u003e\n\u003cli\u003eRemarkably low cytotoxicity ensures cell viability\u003c\/li\u003e\n\u003cli\u003eAffordability that doesn't compromise quality\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003ePackage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 (1.0 mL):\u003c\/span\u003e Suitable for approximately 1,200 reactions when transfecting 0.5~5.0 pmol siRNA or miRNA mimics in a 24-well plate.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Buffer (5X):\u003c\/span\u003e Expertly formulated for maximal transfection efficiency, comes in an 8.0 mL concentrated stock solution that can be used to prepare 40 mL of working solution.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eApplications:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIdeal for siRNA, miRNA mimics, or mRNA transfection\u003c\/li\u003e\n\u003cli\u003eEnables seamless DNA\/siRNA co-transfection\u003c\/li\u003e\n\u003cli\u003ePerfect for DNA oligo transfection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv style=\"padding-left: 30px;\" data-mce-style=\"padding-left: 30px;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e: Store at 4 °C\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\" data-mce-style=\"padding-left: 30px;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e Buffer (5X)\u003c\/span\u003e: Store at room temperature (RT)\u003c\/div\u003e\n\u003cp\u003eWhen stored correctly, the product maintains stability for at least 12 months or longer, ensuring long-term usability and reliability.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Moderate Toxicity - siRNA Transfection","offer_id":47197018292555,"sku":"AFG-SNG-TR-018","price":200.0,"currency_code":"USD","in_stock":true},{"title":"5 × 1 mL \/ Moderate Toxicity - siRNA Transfection","offer_id":47197018325323,"sku":"AFG-SNG-TR-019","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-01_4c708614-ed6c-4b5e-a730-7caa0d64f1bd.png?v=1702553989"},{"product_id":"affigen-transfecttm-sirna-in-vitro-2000","title":"AffiGEN® Transfect(TM) siRNA In Vitro 2000","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eIntroducing AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000, a groundbreaking biodegradable non-liposomal solution designed for efficient siRNA\/miRNA and DNA delivery. Powered by our proprietary pH Dependent Conformational Change (PDCC) technology, this innovative transfection reagent sets new standards by incorporating pre-screened hydrophobic groups into its side chain. The result is a versatile and potent gene delivery tool that stands at the forefront of the market.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eKey Features:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUnmatched efficiency in delivering single siRNA\/miRNA or co-transfecting DNA\/siRNA or DNA\/miRNA into a wide range of mammalian cells.\u003c\/li\u003e\n\u003cli\u003eExceptional gene silencing efficacy even at low concentrations, with as little as 1.0 nM siRNA\/miRNA mimics.\u003c\/li\u003e\n\u003cli\u003eMitigates non-specific and off-target effects within the cellular environment.\u003c\/li\u003e\n\u003cli\u003eSimplified one-tube reaction process for ease of use.\u003c\/li\u003e\n\u003cli\u003eIdeal for use across diverse mammalian cell types.\u003c\/li\u003e\n\u003cli\u003eRemarkably low cytotoxicity ensures cell viability.\u003c\/li\u003e\n\u003cli\u003eCost-effective without compromising quality.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003ePackage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000 (1.0 mL):\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e Ample supply for approximately 2,000 reactions, allowing transfection of 5.0 pmol siRNA or miRNA mimics in a 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem; text-decoration-line: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000 Buffer (5X):\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e A carefully formulated solution (8.0 mL) that, when mixed, yields 40 mL of a working solution optimized for maximal transfection efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan style=\"color: #00d0c5;\"\u003e\u003c\/span\u003e\u003cspan style=\"color: #00d0c5;\"\u003eApplications:\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for single siRNA transfection or co-transfection of DNA\/siRNA into various mammalian cells.\u003c\/li\u003e\n\u003cli\u003eTailored for single miRNA, miRNA mimics, or inhibitors transfection, as well as co-transfection of DNA\/miRNA or DNA\/miRNA mimics into a diverse array of mammalian cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\" style=\"font-size: 0.875rem;\"\u003e siRNA In Vitro 2000\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e: Store at 4°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\" style=\"font-size: 0.875rem;\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\" style=\"font-size: 0.875rem;\"\u003e siRNA In Vitro 2000\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e Buffer (5X): Store at room temperature (RT).\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003c\/div\u003e\n\u003cp\u003eWith proper storage, the product maintains stability for at least 12 months or longer, ensuring reliability and longevity. \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 2000\u003c\/span\u003e is your go-to solution for precise and efficient gene delivery in your research.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eStandard Protocols:\u003c\/span\u003e\u003c\/h3\u003e\n\u003ch4\u003eProtocol I: siRNA Transfection per Cell Culture Vessel\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e1. Preparation of Working Solution (1X):\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDilute the \u003cstrong\u003eTransfection Buffer (5X)\u003c\/strong\u003e with ddH2O at a 1:4 ratio to obtain a \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e. Store at room temperature for up to 24 months.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e2. Cell Seeding:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePlate cells 18 to 24 hours prior to transfection to achieve approximately 50% confluency at the time of transfection.\u003c\/li\u003e\n\u003cli\u003eAdd complete medium containing serum and antibiotics to each vessel 30-60 minutes before transfection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e3. siRNA Transfection:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRecommended siRNA concentrations range from 1 to 100 nM. Start with 5.0 nM siRNA for most adherent cell lines, adjusting to 50 nM for challenging cell types.\u003c\/li\u003e\n\u003cli\u003eFollow these steps per vessel:\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ea. Add complete medium with serum and antibiotics.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003eb. Dilute siRNA into the \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e and mix.\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003ec. Add the \u003cstrong\u003eTransfection Reagent\u003c\/strong\u003e to the siRNA solution and mix gently.\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003ed. Incubate for approximately 15 minutes at room temperature.\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003ee. Add the transfection mix drop-wise to the cells.\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003ef. Replace transfection medium with growth medium approximately 5 hours post-transfection if needed.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e \u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e4. Assessment:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEvaluate gene silencing 24-72 hours after transfection using appropriate assays. Adjust conditions based on cell type and experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eProtocol II: DNA\/siRNA Co-transfection:\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e1. Preparation of Working Solution (1X):\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDilute the \u003cstrong\u003eTransfection Buffer (5X)\u003c\/strong\u003e with ddH2O at a ratio of 1:4 to create a stable \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e. Store at room temperature for up to 24 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e Maintain the \u003cstrong\u003eTransfection Buffer (5X)\u003c\/strong\u003e at room temperature to prevent precipitation. Refrigeration may induce white precipitates, which do not compromise transfection efficiency. Upon dilution to \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e, any precipitates will dissolve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e2. Cell Seeding:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePlate cells 18 to 24 hours before transfection to achieve approximately 70% confluency at the time of transfection.\u003c\/li\u003e\n\u003cli\u003eAdd complete medium containing serum and antibiotics to each vessel 30-60 minutes before transfection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e The \u003cstrong\u003eTransfection Reagent\u003c\/strong\u003e is compatible with serum and antibiotics throughout the experiment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e3. DNA \u0026amp; siRNA Co-transfection Protocol:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRecommended for each vessel: Use 0.3-0.5 µg DNA and 1-20 nM siRNA, starting with 0.5 µg DNA and 10 pmoles siRNA (final concentration 10 nM) per vessel.\u003c\/li\u003e\n\u003cli\u003eFollow these steps per vessel:\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ea.\u003c\/strong\u003e Add complete medium with serum and antibiotics.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eb.\u003c\/strong\u003e Dilute 0.5 µg DNA and 10 pmoles siRNA into 100 µL of the  \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e and mix thoroughly.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e Use the \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e for optimal transfection efficiency and maximal gene silencing.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ec.\u003c\/strong\u003e Prepare siRNA stock solution at 10 µM and add 1.0 µL per vessel for a final 10 nM siRNA concentration.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ed.\u003c\/strong\u003e Add 3 µL of the \u003cstrong\u003eTransfection Reagent\u003c\/strong\u003e immediately and mix gently.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003ee.\u003c\/strong\u003eIncubate for approximately 15 minutes at room temperature to allow transfection complex formation. Do not exceed 30 minutes.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ef.\u003c\/strong\u003e Add the transfection complex drop-wise to the cells.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eg.\u003c\/strong\u003e Gently rock the vessel and return it to the incubator.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eh.\u003c\/strong\u003e Replace the transfection medium with cell growth medium approximately 5 hours post-transfection if necessary.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e \u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003e4. Assessment:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAssess gene silencing 24-72 hours post-transfection. Adjust parameters based on cell type and experimental requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eProtocol III: DNA transfection to Mammalian Cells\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e1. Preparation of Working Solution (1X):\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDilute the \u003cstrong\u003eTransfection Buffer (5X)\u003c\/strong\u003e with ddH2O at a ratio of 1:4 to create a stable \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e. Store at room temperature for up to 24 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e Maintain the \u003cstrong\u003eTransfection Buffer (5X)\u003c\/strong\u003e at room temperature to prevent precipitation. Refrigeration may induce white precipitates, which do not compromise transfection efficiency. Upon dilution to \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e, any precipitates will dissolve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e2. Cell Seeding:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePlate cells 18 to 24 hours before transfection to achieve approximately 80% confluency at the time of transfection. Add complete medium containing serum and antibiotics to each vessel 30-60 minutes before transfection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e the \u003cstrong\u003eTransfection Rreagent\u003c\/strong\u003e is compatible with serum and antibiotics throughout the experiment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e3. DNA Transfection Protocol:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRecommended for each vessel: Use 0.5 to 1.0 µg DNA per well in a 6-well plate. Starting with 1.0 µg DNA and 3.0 µL of the \u003cstrong\u003eTransfection Reagent\u003c\/strong\u003e per well typically yields satisfactory efficiency without visible toxicity.\u003c\/li\u003e\n\u003cli\u003eFollow these steps per vessel:\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ea.\u003c\/strong\u003e Add 1.0 mL of complete medium with serum and antibiotics to each well 30-60 minutes before transfection.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eb.\u003c\/strong\u003e Dilute 1.0 µg DNA into 100 µL of the \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e and mix thoroughly.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e Use the \u003cstrong\u003eWorking Solution (1X)\u003c\/strong\u003e for optimal transfection efficiency and maximal gene expression.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ec.\u003c\/strong\u003e Add 3 µL of the \u003cstrong\u003eTransfection Reagent\u003c\/strong\u003e and vortex briefly.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ed.\u003c\/strong\u003e Incubate for approximately 10 minutes at room temperature to allow transfection complex formation. Do not exceed 20 minutes.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ee.\u003c\/strong\u003e Add the transfection complex drop-wise to the cells.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003ef.\u003c\/strong\u003e Gently rock the vessel and return it to the incubator.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 40px;\"\u003e\n\u003cstrong\u003eg.\u003c\/strong\u003e Replace the transfection medium with cell growth medium 24 hours after transfection and proceed with analysis as required. h. Adjust parameters based on cell type and experimental requirements.\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Moderate Toxicity - siRNA miRNA \u0026 DNA Transfection","offer_id":47197137633611,"sku":"AFG-SNG-TR-020","price":250.0,"currency_code":"USD","in_stock":true},{"title":"5 × 1 mL \/ Moderate Toxicity - siRNA miRNA \u0026 DNA Transfection","offer_id":47197137666379,"sku":"AFG-SNG-TR-021","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-03_13f3350b-9fab-4643-a3d8-60c77285b257.png?v=1702555046"},{"product_id":"affigen-transfecttm-sirna-in-vitro-3000","title":"AffiGEN® Transfect(TM) siRNA In Vitro 3000","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eRevolutionary Liposome-Based RNA Delivery System\u003c\/p\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000 is at the forefront of RNA delivery technology, offering an advanced liposome formulation designed to optimize in vitro RNA transfection. It represents a leap forward in the field, providing researchers with an exceptionally powerful tool for their experiments.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eKit Composition and Volume:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000:\u003c\/span\u003e Comes in a 1.0 ml volume, enough to perform approximately 1330 transfections, assuming the use of 5.0 pmol siRNA per reaction in a 24-well plate format.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000 Buffer:\u003c\/span\u003e An 8.0 ml buffer, engineered to enhance transfection efficiency to its maximum potential.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage Guidelines:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000:\u003c\/span\u003e Should be stored at a constant 4 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000 Buffer:\u003c\/span\u003e Keep at room temperature (RT) and note it's provided in a 5x concentrate form.\u003c\/p\u003e\n\u003cp\u003eAdherence to these storage conditions ensures the kit remains effective for 12 months or even longer, maintaining its stability and efficacy.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eDistinguished Advantages:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThe \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 3000\u003c\/span\u003e distinguishes itself through its cutting-edge lipid-conjugation technology and proprietary formulation, setting a new standard in the RNA delivery domain:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eUnmatched Efficiency:\u003c\/span\u003e Engineered to deliver siRNA with exceptional efficiency across a wide array of mammalian cell types, including those that are traditionally challenging to transfect. It achieves this with remarkably low cytotoxicity, safeguarding cell viability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eCost Efficiency:\u003c\/span\u003e Offers a more economical option without compromising on quality or effectiveness, making high-quality transfection accessible to more researchers.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eAdditional Benefits:\u003c\/span\u003e This reagent excels in various aspects that enhance its usability and effectiveness:\u003cbr\u003e\n\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003eResistance to sera interference, ensuring consistent and reliable transfection outcomes.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eHigh stability for dependable performance over time.\u003c\/li\u003e\n\u003cli\u003eA straightforward and rapid procedure, facilitating ease of use.\u003c\/li\u003e\n\u003cli\u003eIdeal for high-throughput applications, allowing for scalable research projects.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003cp\u003eElevate your research with AffiGEN® TransfectTM siRNA In Vitro 3000 — the epitome of efficiency and innovation in RNA delivery.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Low Toxicity - DNA \u0026 siRNA Transfection","offer_id":47197153067339,"sku":"AFG-SNG-TR-022","price":197.0,"currency_code":"USD","in_stock":true},{"title":"5 x 1 mL \/ Low Toxicity - DNA \u0026 siRNA Transfection","offer_id":47197153100107,"sku":"AFG-SNG-TR-023","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-10_8a773bc4-1a54-445a-9205-23eb79802559.png?v=1702555188"},{"product_id":"affigen-transfecttm-sirna-in-vitro-4000","title":"AffiGEN® Transfect(TM) siRNA In Vitro 4000","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eRevolutionary Hybrid Formulation for Optimal siRNA Delivery\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000 stands at the forefront of genetic research tools, thanks to its innovative hybrid composition that combines the best aspects of liposome and polymer technologies. This cutting-edge approach ensures not only superior siRNA delivery efficiency but also minimal to no cytotoxicity, setting a new standard in the field.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eContents and Specifications:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000:\u003c\/span\u003e 1.0 mL, designed for approximately 1330 reactions, assuming the use of 5.0 pmol siRNA per reaction in a 24-well plate format.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000 Buffer (5X):\u003c\/span\u003e 8.0 mL, engineered to enhance transfection efficiency, yields 40 mL of ready-to-use solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage Guidelines:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-fragment=\"1\" data-mce-style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000:\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eRefrigerate at 4 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-fragment=\"1\" data-mce-style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eBuffer (5X):\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eStore at room temperature (RT).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eAdhering to these conditions will ensure the product remains stable and effective for 12 months or more.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eDistinguished Advantages:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eLeveraging proprietary technologies in lipid-conjugation and polymer synthesis, \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 4000\u003c\/span\u003e distinguishes itself from competitors with several key benefits:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eUnmatched Efficiency:\u003c\/span\u003e Ideal for transfecting cells that are traditionally challenging, ensuring high-level performance across a diverse range of mammalian cells, all while maintaining virtually undetectable cytotoxicity levels.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eCost-Effectiveness:\u003c\/span\u003e Offers a superior, budget-friendly alternative without compromising on quality or efficacy.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"color: #000000;\"\u003eAdditional Benefits:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003eFree from sera interference, facilitating consistent and reliable results.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003eDemonstrates exceptional stability for long-term storage and use.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003eSimplified and expedited protocol, saving valuable time in the lab.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #000000;\"\u003eFully compatible with high-throughput screening methods, making it perfect for extensive research projects.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 4000\u003c\/span\u003e merges unparalleled efficiency with ease of use, making it an indispensable tool for researchers aiming to push the boundaries of genetic discovery.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Very Low Toxicity - siRNA Transfection","offer_id":47197172957515,"sku":"AFG-SNG-TR-024","price":303.0,"currency_code":"USD","in_stock":true},{"title":"5 X 1 mL \/ Very Low Toxicity - siRNA Transfection","offer_id":47197172990283,"sku":"AFG-SNG-TR-025","price":1064.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-11_6e247880-5e37-4037-bb83-7c11793ca1bb.png?v=1702555356"},{"product_id":"affigen-transfecttm-sirna-in-vitro-1000-plus","title":"AffiGEN® Transfect(TM) siRNA In Vitro 1000 Plus","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eAdvanced Formulation for Unrivaled Gene Delivery Performance\u003c\/p\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus represents a significant leap forward in gene delivery technology. By integrating pre-screened hydrophobic amino acids into a peptide backbone that spans 36 amino acids in length, this reagent leverages our proprietary pH-Dependent Conformational Change (PDCC) technology. This innovation enables the \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000 Plus\u003c\/span\u003e to self-assemble during the formation of the transfection complex, establishing it as an exceptionally versatile and potent tool for gene delivery. Demonstrating over 95% silencing efficiency with just 1 nM siRNA across a wide range of mammalian cells, \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000 Plus\u003c\/span\u003e sets a new benchmark for efficiency and versatility in gene transfection.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eContents and Specifications:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus:\u003c\/span\u003e 1.0 mL, enough for approximately 1000 reactions, with each reaction designed for transfecting 2.5 pmol siRNA in a 24-well plate.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus Buffer (5X):\u003c\/span\u003e 8.0 mL, optimized to ensure maximum transfection efficiency, yielding 40 mL of working solution.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage Recommendations:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus:\u003c\/span\u003e Refrigerate at 4 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus Buffer (5X):\u003c\/span\u003e Keep at room temperature (RT).\u003c\/p\u003e\n\u003cp\u003eWhen stored under the recommended conditions, the product will maintain its stability and effectiveness for 12 months or more.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eKey Advantages:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eExceptional Efficiency:\u003c\/span\u003e Achieves up to 95% gene silencing with as little as 0.5 pmol of siRNA (equivalent to 1.0 nM siRNA in a 24-well plate), setting a high standard for performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eDesigned for Challenging Cells:\u003c\/span\u003e Formulated specifically to transfect mammalian cells that are typically resistant to conventional methods.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eSimplified Protocol:\u003c\/span\u003e Offers a straightforward, one-tube reaction process, making transfections easier and more convenient than ever.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eBroad Applicability:\u003c\/span\u003e Excelling across a wide spectrum of mammalian cells, including those that are notoriously hard to transfect, ensuring broad utility in research.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"text-decoration: underline;\"\u003eMinimal Cytotoxicity:\u003c\/span\u003e Maintains cell viability and health, thanks to its very low cytotoxic profile.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus is the culmination of advanced research and development, designed to offer researchers unparalleled efficacy, ease of use, and versatility in genetic manipulation. Whether for routine laboratory applications or cutting-edge genetic research, PepMute™ Plus provides a robust solution for achieving high-efficiency DNA and siRNA delivery with minimal impact on cell health.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL \/ Moderate Toxicity - siRNA \u0026 DNA Transfection","offer_id":47197185376587,"sku":"AFG-SNG-TR-026","price":200.0,"currency_code":"USD","in_stock":true},{"title":"5 × 1 mL \/ Moderate Toxicity - siRNA \u0026 DNA Transfection","offer_id":47197185409355,"sku":"AFG-SNG-TR-027","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/transfect-01_bd5705d4-4c61-439c-8043-c32ef8fd5b4f.png?v=1702555448"},{"product_id":"affigen-transfecttm-sirna-in-vitro-1000-buffer-5x","title":"AffiGEN® Transfect(TM) siRNA In Vitro 1000 Buffer (5X)","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Buffer (5X)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eIntroducing our cutting-edge Advanced AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Buffer (5X), precisely formulated to achieve optimal transfection efficiency and maximize gene expression knockdown when paired with \u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000\u003c\/span\u003e\u003c\/a\u003e and \u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000 Plus\u003c\/span\u003e\u003c\/a\u003e. This innovative buffer is your key to success when diluting siRNA\/DNA and combining them with \u003ca data-mce-fragment=\"1\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" data-mce-href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000\u003c\/span\u003e\u003c\/a\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\"\u003e(Plus)\u003c\/a\u003e\u003c\/span\u003e to create the most efficient transfection complex.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eProduct Details:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003eProduct Name:\u003c\/span\u003e \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 1000 Buffer (5X)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003eVolume:\u003c\/span\u003e Each vial contains 8.0 mL of 5X concentrated stock solution, enabling you to prepare 40 mL of working solution at a 1X concentration.\u003c\/p\u003e\n\u003cp\u003eSufficient for up to 1000 transfections in a standard 24-well plate.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eStore at room temperature (RT).\u003c\/p\u003e\n\u003cp\u003eWith proper storage, this product maintains stability for a minimum of 12 months, and potentially even longer.\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47197216735563,"sku":"AFG-SNG-TR-037","price":102.75,"currency_code":"USD","in_stock":true},{"title":"4 mL","offer_id":47197216768331,"sku":"AFG-SNG-TR-038","price":111.0,"currency_code":"USD","in_stock":true},{"title":"8 mL","offer_id":47197216801099,"sku":"AFG-SNG-TR-039","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen-transfect-buffer_b8dece23-bbe0-40f1-9f0d-b7ba2523750b.png?v=1702555661"},{"product_id":"affigen-transfecttm-sirna-in-vitro-2000-buffer-5x","title":"AffiGEN® Transfect(TM) siRNA In Vitro 2000 Buffer (5X)","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000 Buffer (5X)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eOur Enhanced AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000 Buffer (5X) has been specially formulated to ensure the highest level of transfection efficiency and optimal gene expression knockdown when used in conjunction with our \u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e. This advanced buffer is designed for diluting siRNA\/DNA and our \u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 2000\u003c\/span\u003e\u003c\/a\u003e, resulting in the formation of the most efficient transfection complex possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eProduct Details:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eProduct Name:\u003c\/span\u003e \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 2000 Buffer (5X)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eSize:\u003c\/span\u003e 8.0 mL\/vial, to prepare 40 mL of working solution (1X concentration)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eSufficient for 1000 transfections in a 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eStore at room temperature (RT).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eWhen stored properly, this product maintains stability for at least 12 months or longer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47197229711691,"sku":"AFG-SNG-TR-028","price":102.75,"currency_code":"USD","in_stock":true},{"title":"4 mL","offer_id":47197229744459,"sku":"AFG-SNG-TR-029","price":111.0,"currency_code":"USD","in_stock":true},{"title":"8 mL","offer_id":47197229777227,"sku":"AFG-SNG-TR-030","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen-transfect-buffer_1b248a68-8497-43e6-8e01-7f317447464b.png?v=1702555767"},{"product_id":"affigen-transfecttm-sirna-in-vitro-3000-buffer-5x","title":"AffiGEN® Transfect(TM) siRNA In Vitro 3000 Buffer (5X)","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000 Buffer (5X)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eIntroducing our cutting-edge Advanced \u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e Buffer (5X), thoughtfully crafted to elevate transfection efficiency to its pinnacle and achieve unparalleled gene expression knockdown. This extraordinary buffer is exclusively designed to complement our \u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003esiRNA In Vitro 3000\u003c\/a\u003e, ensuring optimal results when diluting siRNA\/DNA and combining them with \u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\"\u003eAffiGEN® Transfect\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003esiRNA In Vitro 3000\u003c\/a\u003e to form the most effective transfection complex.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eProduct Details:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eProduct Name:\u003c\/span\u003e \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 3000 Buffer (5X)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eVolume:\u003c\/span\u003e Each vial contains 8.0 mL of 5X concentrated stock solution, yielding 40 mL of working solution at 1X concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eCapable of facilitating up to 1,200 transfections in a standard 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eStore at room temperature (RT).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003eWhen stored under proper conditions, this product maintains stability for a minimum of 12 months, and potentially even longer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47197256745291,"sku":"AFG-SNG-TR-034","price":102.75,"currency_code":"USD","in_stock":true},{"title":"4 mL","offer_id":47197256778059,"sku":"AFG-SNG-TR-035","price":111.0,"currency_code":"USD","in_stock":true},{"title":"8 mL","offer_id":47197256810827,"sku":"AFG-SNG-TR-036","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen-transfect-buffer_4c2c63ab-3811-44b4-b9b2-4cc0dd340004.png?v=1702555915"},{"product_id":"affigen-transfecttm-sirna-in-vitro-4000-buffer-5x","title":"AffiGEN® Transfect(TM) siRNA In Vitro 4000 Buffer (5X)","description":"\u003ch2\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000 Buffer (5X)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eOur Enhanced AffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000 Buffer (5X) has been meticulously formulated to achieve the highest level of transfection efficiency and maximal gene expression knockdown, specifically optimized for use with our \u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN®\u003c\/span\u003e \u003cspan data-mce-fragment=\"1\"\u003eTransfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 4000\u003c\/span\u003e\u003c\/a\u003e. This advanced buffer is the ideal solution for diluting siRNA\/DNA and the \u003ca title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\" href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eTransfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 4000\u003c\/span\u003e\u003c\/a\u003e, ensuring the formation of the most efficient transfection complex.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eProduct Details:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eProduct Name:\u003c\/span\u003e \u003cspan data-mce-fragment=\"1\"\u003eAffiGEN® Transfect\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003eTM\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e siRNA In Vitro 4000 Buffer (5X)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003eVolume:\u003c\/span\u003e 8.0 mL per vial, yielding a 40 mL working solution at 1X concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eDesigned for up to 1000 transfections in a standard 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eStorage:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eStore at room temperature (RT).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eUnder proper storage conditions, this product maintains stability for a minimum of 12 months and potentially even longer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan style=\"color: #00d0c5;\" data-mce-style=\"color: #00d0c5;\"\u003esiRNA In Vitro Transfection Reagents Specifications and Guide Application\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000\" title=\"AffiGEN® Transfect(TM) siRNA In Vitro 1000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-2000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 2000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 2000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-3000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 3000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 3000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-4000\" title=\"AffiGEN® TransfectTM siRNA In Vitro 4000\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 4000\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e\u003ca href=\"https:\/\/affigen.com\/products\/affigen-transfecttm-sirna-in-vitro-1000-plus\" title=\"AffiGEN® TransfectTM siRNA In Vitro 1000 Plus\"\u003eAffiGEN® Transfect\u003csup\u003eTM\u003c\/sup\u003e siRNA In Vitro 1000 Plus\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eNucleic Acid\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA, miRNA, DNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003esiRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTechnology\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eNon-liposomal\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLiposome formulation\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePolymer-based\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003ePeptide-based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eEfficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eHigh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eOff-target effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCytotoxicity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eLow\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eVery Low\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eModerate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003ePackage sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e1 mL, 5X 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003e4°C, Buffer at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, RNAi\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing, miRNA delivery, DNA delivery\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eGene silencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eTarget Organisms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eMammalian cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eCell Types Suitable For\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including suspension cells and difficult-to-transfect cells\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eWide range of cell types, including hard-to-transfect cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cstrong\u003eAdditional Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eCan be used for in vivo applications\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eN\/A\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eOptimized for siRNA delivery, but can also be used for DNA transfection\u003c\/td\u003e\n\u003ctd style=\"width: 18%;\"\u003eEffective at low concentrations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":47197266248011,"sku":"AFG-SNG-TR-031","price":102.75,"currency_code":"USD","in_stock":true},{"title":"4 mL","offer_id":47197266280779,"sku":"AFG-SNG-TR-032","price":111.0,"currency_code":"USD","in_stock":true},{"title":"8 mL","offer_id":47197266313547,"sku":"AFG-SNG-TR-033","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen-transfect-buffer_f7765f53-0439-4813-8e52-d1f5c7d4d6f8.png?v=1702555990"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/collections\/affigen-transfect_0f7f09ab-832e-4088-b590-1153adfa10d2.png?v=1707143444","url":"https:\/\/affigen.com\/it-it\/collections\/sirna-in-vitro-transfection-reagents.oembed","provider":"AffiGEN","version":"1.0","type":"link"}