{"title":"mRNA Vaccine Products","description":"","products":[{"product_id":"affigen-double-stranded-rna-dsrna-elisa-assay-kit","title":"AffiGEN® double-stranded RNA (dsRNA) ELISA Assay Kit","description":"\u003ch2\u003eAffiGEN® double-stranded RNA (dsRNA) ELISA Assay Kit\u003c\/h2\u003e\n\u003cp\u003eThe AffiGEN® dsRNA ELISA Kit is designed to detect and measure double-stranded RNA (dsRNA) contamination in in vitro transcribed (IVT) mRNA products. dsRNA is a common byproduct formed during mRNA production, and its presence can trigger immune responses and reduce the effectiveness of mRNA-based vaccines and therapies. Removing or minimizing dsRNA is essential for developing safe and high-quality mRNA drugs.\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses a highly specific anti-dsRNA antibody that recognizes only double-stranded RNA, without cross-reacting with single-stranded RNA or DNA. It provides reliable, sensitive, and quantitative results, making it useful across different stages of mRNA drug development—from early research and process optimization to final product quality control.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eIntended use:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis kit is designed for the quantitative measurement of residual double-stranded RNA (dsRNA).\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage conditions:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eStore the entire kit at 2–8°C for the duration of its shelf life. To maintain stability, avoid exposure to strong light.\u003c\/li\u003e\n\u003cli\u003eAfter opening the microplate, cover any unused wells with the plate sealer and place it back into the original foil pouch with the desiccant pack. Seal the pouch tightly and return it to 2–8°C as soon as possible.\u003c\/li\u003e\n\u003cli\u003eAll other reagents should also be returned to 2–8°C immediately after use to preserve performance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit components:\u003c\/h3\u003e\n\u003ctable style=\"width: 100%;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 40%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eELISA Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e8x 12 Well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eBiotinylated Detection Antibody (100x)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e120 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eHRP Streptavidin (100x)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e120 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eDilution Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eTMB Substrate Solution\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eConcentrated Wash Buffer (20x)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eStandard (UTP, 5ng\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e15 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eStandard (pUTP, 5ng\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e15 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eStandard (N1-Me-pUTP, 5ng\/μL) \u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e15 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eStandard (5-OMe-UTP, 5ng\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e15 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eSTE Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e4 PCS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.5793%;\"\u003eInstruction Manual \u0026amp; COA\u003c\/td\u003e\n\u003ctd style=\"width: 66.0517%;\"\u003e1 Copy Each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c!----\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eMaterials Required But Not Supplied:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eMicroplate reader with a 450 ± 10 nm filter (preferably with detection capability at both 450 nm and 650 nm wavelengths).\u003c\/li\u003e\n\u003cli\u003eMicroplate shaker.\u003c\/li\u003e\n\u003cli\u003eRNase-free tips and centrifuge tubes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eProcedure workflow:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e1. Prepare reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGet all necessary reagents ready (standards, samples, buffers, DA conjugate, HRP-SA, TMB substrate, stop solution).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e2. Add standards and samples – 100 µL\/well\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePipette 100 µL of both standards and unknown samples into each well of the ELISA plate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e3. Incubate\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAt room temperature (R.T.)\u003c\/li\u003e\n\u003cli\u003e500 rpm shaking\u003c\/li\u003e\n\u003cli\u003eFor 60 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e4. Discard liquid and snap plate on absorbent paper\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInvert the plate to remove contents.\u003c\/li\u003e\n\u003cli\u003eTap the plate firmly on absorbent paper to remove residual fluid.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e5. Wash with 1× wash buffer – 4 times\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWash each well with the buffer.\u003c\/li\u003e\n\u003cli\u003eTypically involves filling, soaking briefly, and discarding 4 times.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e6. Add biotinylated DA – 100 µL\/well\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDispense 100 µL of biotinylated detection antibody (DA) into each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e7. Incubate\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eR.T.\u003c\/li\u003e\n\u003cli\u003e500 rpm shaking\u003c\/li\u003e\n\u003cli\u003eFor 60 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e8. Discard liquid and snap plate on absorbent paper\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRemove unbound detection antibody.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e9. Wash with 1× wash buffer – 4 times\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSame washing procedure to remove any excess unbound detection antibody.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e10. Add HRP-SA – 100 µL\/well\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 100 µL of horseradish peroxidase-streptavidin (HRP-SA) conjugate to each well.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e11. Incubate\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eR.T.\u003c\/li\u003e\n\u003cli\u003e500 rpm shaking\u003c\/li\u003e\n\u003cli\u003eFor 30 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e12. Discard liquid and snap plate on absorbent paper\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRemove unbound HRP-SA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e13. Wash with 1× wash buffer – 4 times\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFinal wash to ensure minimal background noise in detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e14. Add TMB substrate – 100 µL\/well\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 100 µL of tetramethylbenzidine (TMB) solution.\u003c\/li\u003e\n\u003cli\u003eThis reacts with HRP to produce a blue color.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e15. Incubate at R.T. in the dark for 30 minutes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAllow color to develop.\u003c\/li\u003e\n\u003cli\u003eProtect from light to avoid degradation of the TMB.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e16. Add stop solution – 50 µL\/well\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTypically sulfuric acid (H₂SO₄) or another acidic solution to stop the reaction.\u003c\/li\u003e\n\u003cli\u003eThe color changes from blue to yellow.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e17. Read absorbance at OD450\/650 nm\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure the absorbance using a microplate reader.\u003c\/li\u003e\n\u003cli\u003eOD450 is for quantification; OD650 may be used as reference.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"48 Well","offer_id":50087655637323,"sku":"AFG-HZM-132","price":800.0,"currency_code":"USD","in_stock":true},{"title":"96 Well","offer_id":50087655670091,"sku":"AFG-HZM-133","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/affigen_27b3f592-6cbe-4dae-992e-62128fb54f78.png?v=1721733383"},{"product_id":"affigen-lnp-kit-for-fast-in-vivo-rna-delivery","title":"AffiGEN® LNP Kit for Fast in vivo RNA Delivery","description":"\u003ch2\u003eAffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h2\u003e\n\u003cp\u003eThe AffiGEN® LNP Kit for Fast in vivo RNA Delivery is a state-of-the-art, ready-to-use lipid nanoparticle (LNP) encapsulation solution. This innovative product is specifically crafted for efficient in vivo RNA delivery in experimental animals. Utilizing a unique endocytosis delivery and endosomal escape mechanism, the AffiGEN® LNP Kit ensures highly effective delivery of various RNAs, including mRNAs, gRNAs, and saRNAs.\u003c\/p\u003e\n\u003ch3\u003eApplications of AffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene Editing Efficiency Analysis:\u003c\/strong\u003e Enables precise evaluation of gene editing techniques in living organisms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast in vivo RNA Candidate Evaluation:\u003c\/strong\u003e Accelerates the testing of new RNA sequences in animal models.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003emRNA Vaccine and Drug Development:\u003c\/strong\u003e Facilitates the rapid development and testing of RNA-based vaccines and therapeutics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmune Response Stimulation:\u003c\/strong\u003e Ideal for antibody preparation and studying immune responses.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-throughput Screening:\u003c\/strong\u003e Supports large-scale screening of RNA sequences in vivo.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Features of AffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe and Secure:\u003c\/strong\u003e High biosafety with ethanol-free components, reducing potential toxicity and side effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid Screening:\u003c\/strong\u003e Significantly shortens the mRNA screening and validation cycle, providing results faster than traditional methods.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-Use:\u003c\/strong\u003e Simple, efficient mixing protocol minimizes sample loss, allowing encapsulation of mRNA as low as 1μg for in vivo experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExclusive Formulation:\u003c\/strong\u003e Proprietary lipid and formulation technology eliminates patent issues, ensuring unrestricted use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Eliminates the need for specialized encapsulation equipment, saving on both equipment and labor costs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAdministration Routes of AffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntravenous Injection:\u003c\/strong\u003e Ensures systemic distribution, targeting multiple organs efficiently.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntramuscular Injection:\u003c\/strong\u003e Suitable for localized delivery and sustained release in muscle tissue.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntraperitoneal Injection:\u003c\/strong\u003e Ideal for widespread abdominal distribution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSubcutaneous Injection:\u003c\/strong\u003e Provides a slow, sustained release and localized targeting.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eAdvantages of AffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Targeting Efficiency:\u003c\/strong\u003e Demonstrates comparable targeting effects to microfluidic encapsulated LNPs in liver and spleen tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMulti-Mode Compatibility:\u003c\/strong\u003e Compatible with various administration routes, offering flexibility in experimental design.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTime-Saving Protocol:\u003c\/strong\u003e The one-step solution allows for rapid encapsulation and injection, streamlining the workflow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStable and Robust:\u003c\/strong\u003e The formulation is stable under different storage conditions, maintaining efficacy over time.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eTechnical Specifications of AffiGEN® LNP Kit for Fast in vivo RNA Delivery\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLNP Concentration:\u003c\/strong\u003e Optimized for high efficiency in RNA delivery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParticle Size:\u003c\/strong\u003e Uniform and consistent particle size for predictable performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e Stable at 4°C; 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