{"product_id":"affings-universal-plus-dna-library-prep-kit-v4","title":"AffiNGS® Universal Plus DNA Library Prep Kit V4","description":"\u003ch2\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(66, 149, 147);\"\u003eAffiNGS® Universal Plus DNA Library Prep Kit V4\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description : \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEnzyme-based kit for fragmented DNA library preparation designed for next-generation sequencing (NGS) platforms. It is optimized to improve library uniformity and increase tolerance to EDTA and other inhibitors present in DNA sample buffers.\u003c\/p\u003e\n\u003cp\u003eThe workflow integrates DNA fragmentation, end repair, and dA-tailing into a single step, without requiring purification before adapter ligation. Subsequent steps such as adapter ligation, library amplification, and size selection can be performed directly.\u003c\/p\u003e\n\u003cp\u003eIt enables efficient conversion of 1 ng to 1 μg of input DNA into libraries compatible with Illumina and MGI sequencing platforms.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features : \u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh tolerance to impurities such as elevated pH and EDTA, ensuring stable performance in challenging sample conditions.\u003c\/li\u003e\n\u003cli\u003eBroad species compatibility, allowing a unified workflow across different sample types and improving laboratory efficiency.\u003c\/li\u003e\n\u003cli\u003eStrong library uniformity, providing improved coverage of high-GC regions and more consistent sequencing results.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eComponents: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 247.532px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 110.375px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 110.375px;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 110.375px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eAFG-M-0525 \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e(24 Reactions) \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 110.375px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eAFG-M-0526 \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e(96 Reactions)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003eFEA Buffer V4\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e 480 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003eFEA Enzyme Mix V4\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e960 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003eRapid Ligation Buffer V4\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e600 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e 4  x 600 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003eRapid DNA Ligase V4\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e480 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003e Ultra Amplification Mix\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e600 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e4  x 600 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003ePCR Primer Mix for Illumina\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e480 μL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 47.8475%; height: 19.5938px;\"\u003ePCR Primer Mix for MGI\u003c\/td\u003e\n\u003ctd style=\"width: 15.1489%; height: 19.5938px;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 36.4621%; height: 19.5938px;\"\u003e480 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"AffiGEN","offers":[{"title":"24 Reactions","offer_id":57145295864139,"sku":"AFG-M-0525","price":1776.75,"currency_code":"USD","in_stock":true},{"title":"96 Reactions","offer_id":57145307562315,"sku":"AFG-M-0526","price":6112.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiNGS_1500x_ab328002-703b-4d46-a8b5-c155252da16b.png?v=1699871215","url":"https:\/\/affigen.com\/fr-ca\/products\/affings-universal-plus-dna-library-prep-kit-v4","provider":"AffiGEN","version":"1.0","type":"link"}