AffiGen
AffiELISA® Goat IL6 Kit: Enhanced Interleukin 6 ELISA Detection
AffiELISA® Goat IL6 Kit: Enhanced Interleukin 6 ELISA Detection
SKU:AFG-E4666
Regular price
$500.00 USD
Regular price
Sale price
$500.00 USD
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The AffiELISA® Goat IL6 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Interleukin 6 (IL6) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Interleukin 6 (IL6) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Interleukin 6 (IL6) in the samples can be determined.
