{"title":"AffiZYME®","description":"","products":[{"product_id":"affizyme-t4-dna-ligase","title":"AffiZYME® T4 DNA Ligase","description":"\u003cp\u003eAffiZYME® T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in blunt-end or sticky-end dsDNA. This product also can catalyze the ligation of RNA and ssDNA or RNA strands in double strands, but it cannot catalyze the ligation of whole single-stranded nucleotides. It is suitable for nucleic acid operations such as labeling RNA 3'-ends, circularizing RNA and DNA oligonucleotides or cloning cDNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"40,000 U","offer_id":46663597293899,"sku":"AFG-M-0154","price":58.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_2aa1fbf2-ae97-4834-9b44-574db82f884e.png?v=1741089620"},{"product_id":"affizyme-t7-endonuclease-i","title":"AffiZYME® T7 Endonuclease I","description":"\u003cp\u003eAffiZYME® T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5' to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR\/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003e250 U\u003c\/td\u003e\n\u003ctd\u003e1250 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT7 Endonuclease I\u003c\/td\u003e\n\u003ctd\u003e25 μL\u003c\/td\u003e\n\u003ctd\u003e1\u003cspan data-mce-fragment=\"1\"\u003e25 μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT7 Endonuclease I Reaction Buffer (10 ×)\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControl Template*\u003c\/td\u003e\n\u003ctd\u003e10 μL\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30 ~ -15℃ and transport at ≤0℃.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt's applicable for gene mutation, SNP and TALEN or CRISPR\/Cas9-mediated mutation detection.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp\u003eT7 endonuclease I is a structure-selective enzyme which acts on different DNA substrates with different activities.Therefore, it is essential to control the amount of enzyme and reaction time when cleaving a specific substrate. Nonspecific nuclease activity will be increased when the temperature is above 42℃. Control the temperature and make sure it won't exceed 55℃, or the activity of enzyme will be decreased.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"250 U","offer_id":46663605649739,"sku":"AFG-M-0298","price":87.0,"currency_code":"USD","in_stock":true},{"title":"1,250 U","offer_id":46663605682507,"sku":"AFG-M-0299","price":362.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME.png?v=1741089577"},{"product_id":"affizyme-t4-dna-polymerase","title":"AffiZYME® T4 DNA polymerase","description":"\u003ch2\u003eAffiZYME® T4 DNA polymerase\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiZYME® T4 DNA Polymerase\u003c\/strong\u003e catalyzes DNA synthesis in the 5'----\u0026gt;3' direction in the presence templates and primers. This enzyme also has 3'----\u0026gt;5' exonuclease activity which is more active than that of DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase has no 5'----\u0026gt;3' exonuclease activity. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-VZ-N101-01\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 2,000 U\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 DNA polymerase (3 U\/μL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e667 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X Blue Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReaction Buffer:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10X Blue Buffer:\u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003e100 mM Tris-HCI pH 7.9 at 25°C 500 mM NaCl \u003cbr\u003e100 mM MgCb \u003cbr\u003e10 mM DTT\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30°C to -15°C and transport at \u003cspan data-mce-fragment=\"1\"\u003e≤0°\u003c\/span\u003eC.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRemoval of 3' overhangs or fill-in of 5' overhangs to form blunt ends.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant E.coli strains carrying the cloned T4 bacteriophage DNA polymerase gene. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOne unit of activity (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble precipitates within 30 min at 37°C. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp\u003eIt is recommended to place the enzyme on ice during use and put it back to -20°C immediately after use .\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"2,000 U","offer_id":51385848299851,"sku":"AFG-M-0162","price":446.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_1_1500x_1500x_bba3ccb9-6e9a-4b41-9e36-50486cae8026.png?v=1741089521"},{"product_id":"affi-t4-polynucleotide-kinase","title":"AffiZYME® T4 Polynucleotide Kinase","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiZYME® T4 Polynucleotide Kinase\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiZYME® T4 Polynucleotide Kinase\u003c\/strong\u003e catalyzes the transfer of the y-phosphate from ATP to a 5'-hydroxy terminus (double-stranded or single-stranded DNA or RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also has 3'-phosphatase activity. which can hydrolyze 3'-phosphoryl groups from oligonucleotide 3'-phospahte termini, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates.\u003c\/p\u003e\n\u003cp\u003e \u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1000\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" style=\"text-align: left;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\" class=\"variant-sku\"\u003eAFG-VZ-\u003c\/span\u003eN102-01 10,000 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 Polynucleotide Kinase (10 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X T4 PNK Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReaction Buffer:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10X T4 PNK Buffer :\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\" data-mce-style=\"padding-left: 30px;\"\u003e700 mM Tris-HCI pH 7.6 @ 25°C\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\" data-mce-style=\"padding-left: 30px;\"\u003e100 mM MgCI\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\" data-mce-fragment=\"1\" data-mce-style=\"padding-left: 30px;\"\u003e50 mM DTT\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore t -30 ~-15° and transport at≤0°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10,000 U","offer_id":51385848201547,"sku":"AFG-M-0161","price":583.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_099797de-3dcc-49fc-8ae0-af89014dbc53.png?v=1741089521"},{"product_id":"affi-dna-polymerase-i-klenow-fragment","title":"AffiZYME® DNA polymerase I Klenow fragment","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiZYME® DNA polymerase I Klenow fragment\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThe marge (Klenow) fragment of AffiZYME®DNA polymerase I\u003c\/strong\u003e is a proteolytic product of E.coli DNA polymerase I that has the 5'→3' exonuclease activity. Klenow retains the high fidelity of the holoenzyme without degrading 5' termini.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents: \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"454\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"276\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"178\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\" class=\"variant-sku\"\u003e AFG-VZ-\u003c\/span\u003eN 104-01 5,000 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKlenow Fragment (5 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e1 mlL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X Blue Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e2 x 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReaction Buffer:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10X Blue Buffer :\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e100 mM Tris-HCI pH7.9 @25°C\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e500 mM NaCI\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e100 mM MgCI\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e10 mM DTT\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at~15°C and transport at ≤0°C.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5,000 U","offer_id":51385848136011,"sku":"AFG-M-0160","price":790.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_e4642704-9444-48b7-9c6b-551b9930567d.png?v=1741089520"},{"product_id":"affizyme-dna-polymerase-i-klenow-fragment-exo","title":"AffiZYME® DNA polymerase I Klenow fragment exo-","description":"","brand":"AffiGEN","offers":[{"title":"5,000 U","offer_id":51385848037707,"sku":"AFG-M-0159","price":790.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiGEN_1500x_4eae5014-d966-4fc3-b6ad-a01e04253701.png?v=1741089519"},{"product_id":"affi-t4-dna-ligase-1","title":"AffiZYME® T4 DNA Ligase","description":"\u003ch2 style=\"text-align: left;\"\u003e\u003cspan style=\"color: #429593;\"\u003eAffiZYME® T4 DNA Ligase\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffiZYME® T4 DNA Ligase\u003c\/strong\u003e catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. The enzyme can catalyze the ligation between blunt ends, and repair the single-stranded nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: left;\" width=\"30%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"30%\" style=\"text-align: center;\"\u003e\u003cstrong\u003eAFG-M-0407 \/ 600,000 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 DNA Ligase (Rapid) (600 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2X Rapid Ligation Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X T4 DNA Ligase Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30 ~ -15°C and transport at ≤0°C\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"600,000 U","offer_id":46664629387595,"sku":"AFG-M-0407","price":936.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiNGS_dd2e17d3-fa93-46a0-bbaf-1cf24b4cd68e.png?v=1741089489"},{"product_id":"affizyme-glucose-6-phosphate-dehydrogenase","title":"AffiZYME® Glucose-6-phosphate Dehydrogenase","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAffiZYME® Glucose-6-phosphate Dehydrogenase\u003c\/strong\u003e is a powerful enzyme with high specificity for the glucose-6-phosphate substrate. This enzyme has been designed to exhibit maximum activity for glucose-6-phosphate, providing over 95% efficiency. Utilize this enzyme to achieve reliable results for metabolic studies.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAbbreviation: \u003c\/strong\u003eG6PDH\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 KU","offer_id":46853653233995,"sku":"AFG-HZM-002-10KU","price":356.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":47637364638027,"sku":"AFG-HZM-002-100KU","price":615.0,"currency_code":"USD","in_stock":true},{"title":"500 KU","offer_id":47637364670795,"sku":"AFG-HZM-002-500KU","price":2060.0,"currency_code":"USD","in_stock":true},{"title":"1 000 KU","offer_id":47637364703563,"sku":"AFG-HZM-002-1000KU","price":3290.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_1.png?v=1693131770"},{"product_id":"affizyme-creatinine-amidohydrolase","title":"AffiZYME® Creatinine 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\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"3 KU","offer_id":46853768610123,"sku":"AFG-HZM-026-3KU","price":350.0,"currency_code":"USD","in_stock":true},{"title":"50 KU","offer_id":47637586772299,"sku":"AFG-HZM-026-50KU","price":785.0,"currency_code":"USD","in_stock":true},{"title":"500 KU","offer_id":47637586805067,"sku":"AFG-HZM-026-500KU","price":5480.0,"currency_code":"USD","in_stock":true},{"title":"1 000 KU","offer_id":47637586837835,"sku":"AFG-HZM-026-1000KU","price":8990.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_cf6725c7-dfe3-4b23-b74d-fd37c25a2a91.png?v=1693137190"},{"product_id":"affizyme-lactate-oxidase","title":"AffiZYME® Lactate Oxidase","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAffiZYME® Lactate Oxidase\u003c\/strong\u003e \u003c\/p\u003e\n \u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAbbreviation: 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This convenient liquid format enables efficient enzyme delivery, perfect for a range of molecular biology applications.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e20 mg\/mL\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eNote: \u003c\/strong\u003e5mL is sample size，specific protein activity≥800U\/mL，concentration is 20mg\/mL, No Nickase residue\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":46854341427531,"sku":"AFG-HZM-055","price":300.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":46854341460299,"sku":"AFG-HZM-056","price":350.0,"currency_code":"USD","in_stock":true},{"title":"1000 mL","offer_id":46854341493067,"sku":"AFG-HZM-057","price":303.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":46854341525835,"sku":"AFG-HZM-058","price":301.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_f66c3abb-07ae-4b7d-b658-5fcd6d0088b3.png?v=1693154547"},{"product_id":"affizyme-proteinase-k-mngs-liquid","title":"AffiZYME® Proteinase K (mNGS) (liquid)","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAffiZYME® α-L-Fucosidase\u003c\/strong\u003e \u003c\/p\u003e\n \u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e20 mg\/mL\u003c\/p\u003e\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong\u003eNote: \u003c\/strong\u003elowest nucleic acid residue\u003c\/p\u003e\n \u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"1 mL","offer_id":46854341591371,"sku":"AFG-HZM-059","price":300.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":46854341624139,"sku":"AFG-HZM-060","price":300.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":46854341656907,"sku":"AFG-HZM-061","price":303.0,"currency_code":"USD","in_stock":true},{"title":"1000 mL","offer_id":46854341689675,"sku":"AFG-HZM-062","price":307.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_612fd21d-1bd7-4d5b-a5fe-65d8c010ce99.png?v=1693154550"},{"product_id":"affizyme-rnase-a-100-mg-ml","title":"AffiZYME® RNase A (100 mg\/mL)","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiZYME®  RNase A (100 mg\/mL)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eRNase A is a high-quality endoribonuclease extracted from bovine pancreas that can specifically degrade single-stranded RNA at the C and U residue positions. The enzyme can cleave the phosphodiester bond between the 5' - ribose on the nucleoside and the phosphate group on the 3' - ribose of the adjacent pyrimidine nucleoside, and products are 3' - pyrimidine mononucleotide or oligonucleotide ending in 3' - pyrimidine nucleotide. It is widely used to remove RNA from DNA samples.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\" width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd height=\"21\" class=\"xl66\" width=\"96\" style=\"height: 15.75pt; width: 19.8642%;\"\u003e\u003cstrong\u003eAFG-M-0103\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eRNase A (100 mg\/mL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 15 ~ 25°C for three months, and store at -30 ~ -15°C for long-term storage. Transport at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003eIt can be used to remove RNA during plasmid and genomic DNA preparation.\u003c\/li\u003e\n\u003cli\u003eIt can be used to remove RNA in solution during the preparation of recombinant protein.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eFor research use only. 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This leads to cell wall rupture and bacteriolysis in the end.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\" style=\"width: 79.7963%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 15.75pt; width: 19.8642%;\" width=\"96\" class=\"xl66\" height=\"21\"\u003e\u003cstrong\u003e   AFG-M-0104\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eLysozyme\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e200 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eLysozyme Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eStore Lysozyme dry powder at 2 ~ 8°C and transport at room temperature. Store the other components at room temperature (15 ~ 25°C) and transport at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eMainly for the digestion of Gram-positive bacteria cell walls during DNA and RNA preparation.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"200 mg","offer_id":46856657207627,"sku":"AFG-M-0104","price":18.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_ef47ee94-0528-4c78-9f6d-05ecbbef1880.png?v=1741089464"},{"product_id":"affizyme-gold-t4-dna-ligase-5-u-ul-afg-ysn-061","title":"AffiZYME® Gold T4 DNA Ligase (5 U\/µL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAffiZYME® Gold T4 DNA Ligase can catalyze the formation of phosphodiester bonds at the 5' phosphate end and 3' hydroxyl end of adjacent nucleic acids at blunt or sticky ends of dsDNA when ATP is used as a coenzyme, and can also catalyze RNA to link with ssDNA or ssRNA in double strands, but cannot catalyze linkages between fully single-stranded nucleotides. This product is suitable for labeling the 3'-end of RNA, circularizing RNA and DNA oligonucleotides, cloning cDNA, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5 x 1000 Units","offer_id":47092038566219,"sku":"AFG-YSN-061","price":276.0,"currency_code":"USD","in_stock":true},{"title":"50000 Units","offer_id":47092038598987,"sku":"AFG-YSN-062","price":801.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_1_1500x_1500x_0ee78406-fbb3-49bb-8133-de7c9ee3db93.png?v=1700992454"},{"product_id":"affizyme-proteinase-k-afg-ysn-065","title":"AffiZYME® Proteinase K","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eProteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg\/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5 x 100mg","offer_id":47092039745867,"sku":"AFG-YSN-065","price":444.0,"currency_code":"USD","in_stock":true},{"title":"5 x 180mg","offer_id":47092039778635,"sku":"AFG-YSN-066","price":612.0,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":47092039811403,"sku":"AFG-YSN-067","price":204.6,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":47092039844171,"sku":"AFG-YSN-068","price":763.2,"currency_code":"USD","in_stock":true},{"title":"5 g","offer_id":47092039876939,"sku":"AFG-YSN-069","price":2917.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_9b62d3ad-f620-4dd9-8353-ad040ab417d8.png?v=1699869245"},{"product_id":"affizyme-ribonuclease-a-rnase-a-from-bovine-pancreas-afg-ysn-070","title":"AffiZYME® Ribonuclease A (RNase A) from bovine pancreas","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eRibonuclease A (RNase A) is a single-stranded polypeptide containing 4 disulfide bonds with a molecular weight of about 13.7 kDa. RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Specifically, the cleavage recognizes the phosphodiester bond formed by the 5'-ribose of a nucleotide and the phosphate group on the 3'-ribose of the adjacent pyrimidine nucleotide, so that the 2', 3' - Cyclic phosphates are hydrolyzed to the corresponding 3'-nucleoside phosphates (eg, pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-PG) .RNase A is the most active in cleaving single-stranded RNA .Recommended working concentration is 1-100 μ G\/mL, compatible with various reaction systems. Low salt concentration (0-100 mM NaCl) can be used to cut single-stranded RNA, double-stranded RNA, and RNA chains formed by RNA-DNA hybridization. However, at high salt concentration (≥ 0.3 M) , RNase A only specifically cleaves single-stranded RNA.\n RNase A is most commonly used to remove RNA during the preparation of plasmid DNA or genomic DNA. Whether or not DNase is active during the preparation process can easily affect the reaction. The traditional method of boiling in a water bath can be used to inactivate DNase activity. This product does not contain DNase and protease, and does not require heat treatment before use. In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003ePowder\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100mg","offer_id":47092039975243,"sku":"AFG-YSN-070","price":288.6,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":47092040008011,"sku":"AFG-YSN-071","price":1993.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_6e10371b-68dd-4784-83ec-d2aad4f3a608.png?v=1699869248"},{"product_id":"affizyme-murine-rnase-inhibitor-40-u-l-afg-ysn-081","title":"AffiZYME® Murine RNase inhibitor (40 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eMurine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1: 1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (\u0026lt; 1mmol\/L) . This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription\/translation\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 x 2 K Units","offer_id":47092040171851,"sku":"AFG-YSN-081","price":339.0,"currency_code":"USD","in_stock":true},{"title":"10 K Units","offer_id":47092040204619,"sku":"AFG-YSN-082","price":166.8,"currency_code":"USD","in_stock":true},{"title":"20 K Units","offer_id":47092040237387,"sku":"AFG-YSN-083","price":284.4,"currency_code":"USD","in_stock":true},{"title":"100 K Units","offer_id":47092040270155,"sku":"AFG-YSN-084","price":939.6,"currency_code":"USD","in_stock":true},{"title":"1000 K Units","offer_id":47092040302923,"sku":"AFG-YSN-085","price":11494.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_1ceb3268-768a-48d8-a914-4ef5cc705597.png?v=1699869259"},{"product_id":"affizyme-deo-yribonuclease-i-dnase-i-gmp-grade-2-u-l-afg-ysn-086","title":"AffiZYME® Deoxyribonuclease I (DNase I) GMP-grade (2 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eDNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones) . The optimum pH range is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions, such as Co2+, Mn2+, Zn2+, etc. 5 mM Ca2+ can protect the enzyme from being hydrolyzed. In the presence of Mg2+, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn2+, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.\n This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 x 500 Units","offer_id":47092040335691,"sku":"AFG-YSN-086","price":339.0,"currency_code":"USD","in_stock":true},{"title":"5 x 2000 Units","offer_id":47092040368459,"sku":"AFG-YSN-087","price":486.0,"currency_code":"USD","in_stock":true},{"title":"10000 Units","offer_id":47092040401227,"sku":"AFG-YSN-088","price":393.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_e1f552d1-ab31-4417-b4a5-a1a52419abb1.png?v=1699869262"},{"product_id":"affizyme-mrna-cap-2-o-methyltransferase-gmp-grade-50-u-l-afg-ysn-089","title":"AffiZYME® mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U\/μl)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003emRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.\n This product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo\/in vitro.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10000 Units","offer_id":47092040499531,"sku":"AFG-YSN-089","price":183.6,"currency_code":"USD","in_stock":true},{"title":"50000 Units","offer_id":47092040532299,"sku":"AFG-YSN-090","price":620.4,"currency_code":"USD","in_stock":true},{"title":"250000 Units","offer_id":47092040565067,"sku":"AFG-YSN-091","price":2325.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_61a3115e-93a8-41a8-b4b2-80a69319827a.png?v=1699869264"},{"product_id":"affizyme-mrna-vaccinia-capping-enzyme-gmp-grade-10-u-l-afg-ysn-092","title":"AffiZYME® mRNA Vaccinia Capping Enzyme GMP-grade (10 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eEukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It\"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N) . Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP) , S-adenosylmethionine (SAM) , etc.\n This product is produced by GMP process requirements and provided in a liquid form, used for in vivo\/in vitro pre-translation mRNA capping reaction or mRNA 5'end labeling reaction.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"2 K Units","offer_id":47092040597835,"sku":"AFG-YSN-092","price":351.6,"currency_code":"USD","in_stock":true},{"title":"10 K Units","offer_id":47092040630603,"sku":"AFG-YSN-093","price":1258.8,"currency_code":"USD","in_stock":true},{"title":"100 K Units","offer_id":47092040663371,"sku":"AFG-YSN-094","price":8802.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_84b471dd-930a-45dc-931d-6b822286e2f7.png?v=1699869267"},{"product_id":"affizyme-pyrophosphatase-inorganic-gmp-grade-1-u-l-afg-ysn-099","title":"AffiZYME® Pyrophosphatase, Inorganic GMP-grade (1 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eThis product is an inorganic pyrophosphatase derived from recombinant expression in Escherichia coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions. This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 x 10 Units","offer_id":47092042006859,"sku":"AFG-YSN-099","price":339.0,"currency_code":"USD","in_stock":true},{"title":"100 Units","offer_id":47092042039627,"sku":"AFG-YSN-100","price":200.4,"currency_code":"USD","in_stock":true},{"title":"1000 Units","offer_id":47092042072395,"sku":"AFG-YSN-101","price":1107.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_89e1299b-2aa9-4c6d-bac3-f397307c7609.png?v=1699869272"},{"product_id":"affizyme-murine-rnase-inhibitor-gmp-grade-40-u-l-afg-ysn-102","title":"AffiZYME® Murine RNase inhibitor GMP-grade (40 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eRNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C) . Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.) . This product is produced in accordance with GMP process requirements and is provided in liquid form\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eLiquid\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 K Units","offer_id":47092042170699,"sku":"AFG-YSN-102","price":208.8,"currency_code":"USD","in_stock":true},{"title":"20 K Units","offer_id":47092042203467,"sku":"AFG-YSN-103","price":343.2,"currency_code":"USD","in_stock":true},{"title":"100 K Units","offer_id":47092042236235,"sku":"AFG-YSN-104","price":1225.2,"currency_code":"USD","in_stock":true},{"title":"1 M Units","offer_id":47092042269003,"sku":"AFG-YSN-105","price":8298.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_8b3dc622-a009-48ba-a752-dd0c97a96312.png?v=1699869274"},{"product_id":"affizyme-pyrophosphatase-inorganic-gmp-grade-0-1-u-l-afg-ysn-165","title":"AffiZYME® Pyrophosphatase, Inorganic GMP-grade (0.1 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eThis product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions.\n This product is produced by GMP process requirements, and the product is provided in liquid form\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"15 x 1 Units","offer_id":47092202438987,"sku":"AFG-YSN-165","price":234.0,"currency_code":"USD","in_stock":true},{"title":"5 x 10 Units","offer_id":47092202471755,"sku":"AFG-YSN-166","price":612.0,"currency_code":"USD","in_stock":true},{"title":"100 Units","offer_id":47092202504523,"sku":"AFG-YSN-167","price":1015.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiZYME_1_1500x_2000x_cefa4988-bbd8-4394-a36d-b59631ff9113.png?v=1699871102"},{"product_id":"affizyme-murine-rnase-inhibitor-200-u-l-glycerol-free-afg-ysn-168","title":"AffiZYME® Murine RNase inhibitor (200 U\/μL, Glycerol-free)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eMurine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits RNases A, B and C through binding noncovalently in a 1: 1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteines which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (\u0026lt; 1mmol\/L) . Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription\/translation. 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