{"title":"Traditional \u0026 TA Cloning","description":"","products":[{"product_id":"affizyme-t4-dna-ligase","title":"AffiZYME® T4 DNA Ligase","description":"\u003cp\u003eAffiZYME® T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in blunt-end or sticky-end dsDNA. This product also can catalyze the ligation of RNA and ssDNA or RNA strands in double strands, but it cannot catalyze the ligation of whole single-stranded nucleotides. It is suitable for nucleic acid operations such as labeling RNA 3'-ends, circularizing RNA and DNA oligonucleotides or cloning cDNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"40,000 U","offer_id":46663597293899,"sku":"AFG-M-0154","price":58.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiZYME_2aa1fbf2-ae97-4834-9b44-574db82f884e.png?v=1741089620"},{"product_id":"afficlone-5min-universal-ligation-mix","title":"AffiCLONE® 5 min Universal Ligation Mix","description":"\u003cp\u003e5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt end DNA ligation and TA ligation. Buffer with optimized ligation enhancer make the reaction more efficient and convenient. Ligation can be completed in 5 min at 25℃. The product can be used directly to transform many chemically competent cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003cbr\u003eStore at temperatures between -30℃ and -15℃, and ensure transportation at ≤0℃. After thawing, they can be stored at 4℃ for up to three months, maintaining their quality and efficacy.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions (10 µL\/Reaction)","offer_id":46663597359435,"sku":"AFG-M-0155","price":145.0,"currency_code":"USD","in_stock":true},{"title":"100 Reactions (10 µL\/Reaction)","offer_id":46663597392203,"sku":"AFG-M-0156","price":232.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_f2050bf1-16d2-4bf4-9a6d-59667b78de8a.png?v=1741089619"}],"url":"https:\/\/affigen.com\/en-es\/collections\/traditional-ta-cloning.oembed","provider":"AffiGEN","version":"1.0","type":"link"}