{"title":"AffiPURE®","description":"","products":[{"product_id":"affipure-cell-tissue-isolation-mini-kit","title":"AffiPURE® Cell\/Tissue DNA Isolation Mini Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE®  Cell\/Tissue DNA Isolation Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eAffiPURE® Cell\/Tissue DNA Isolation Mini Kit\u003c\/strong\u003e is intended for the extraction of genomic DNA from 1 - 20 mg of animal tissues and less than 5 × 10^6 cultured cells. This kit is based on silica gel column purification technology that eliminates the need for extraction using phenol\/chloroform organic solvents or time-consuming alcohol precipitation. The whole extraction process takes only 30 - 60 min. The obtained DNA is ready for use in enzyme digestion, PCR, library preparation, virus DNA detection, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\u003cstrong\u003eAFG-M-0073\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GB\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1,1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWashing Buffer A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e26 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWashing Buffer B\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE gDNA Columns\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNotes:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBuffer GA:\u003c\/strong\u003e Provide an environment for sample lysis. \u003cbr\u003e\u003cstrong\u003eRNase Solution:\u003c\/strong\u003e Degrade RNA in the sample. \u003cbr\u003e\u003cstrong\u003eWashing Buffer A:\u003c\/strong\u003e Remove proteins and other impurities from genomic DNA. \u003cbr\u003e\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e Elute genomic DNA from the spin column. \u003cbr\u003e\u003cstrong\u003eBuffer GB:\u003c\/strong\u003e Elute Proteinase K and provides an environment for binding. \u003cbr\u003e\u003cstrong\u003eProteinase K:\u003c\/strong\u003e Lyse tissue sample. \u003cbr\u003e\u003cstrong\u003eWashing Buffer B:\u003c\/strong\u003e Remove salt ions from genomic DNA. \u003cbr\u003e\u003cstrong\u003eAffiPURE gDNA Columns:\u003c\/strong\u003e Adsorb genomic DNA. \u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Collect filtrate\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore Proteinase K and RNase Solution at -30 ~ -15°C and transport at room temperature. \u003cbr\u003eStore the other components at 15 ~ 25°C and transport at room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 - 20 mg of animal tissues. \u003cbr\u003e\u0026lt;5 × 10^6 cultured cells. \u003cbr\u003eLiquid samples. \u003cbr\u003e\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100 Reactions","offer_id":46855745110347,"sku":"AFG-M-0073","price":130.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_ba6396b3-6bf5-4dc3-83de-c6f7da226488.png?v=1741089474"},{"product_id":"affipure-bacteria-isolation-mini-kit","title":"AffiPURE® Bacteria DNA Isolation Mini Kit","description":"\u003ch2\u003e\n\u003cspan style=\"color: #528e90;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Bacteria DNA Isolation Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eAffiPURE® Bacteria DNA Isolation Mini Kit\u003c\/strong\u003e is specially designed for genomic DNA isolation from bacteria of various origins (Gram-negative bacteria, Gram-positive bacteria). The kit uses silica gel membrane purification technology. No toxic reagents such as phenol chloroform are needed during the extraction process, and no time-consuming alcohol precipitation is required. It minimizes RNA, impurities, lipids and other inhibitory impurities to the utmost extent. The extracted genomic DNA has high purity and stable quality, and can be used in downstream experiments such as digestion, PCR, and Southern hybridization.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\" style=\"width: 79.7963%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 15.75pt; width: 19.8642%;\" width=\"96\" class=\"xl66\" height=\"21\"\u003e\u003cstrong\u003eAFG-M-0074\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eBuffer GA\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e44 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eProteinase K Dissolve Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e400 uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eBuffer GB\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eBuffer PB\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e26 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eBuffer PW\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eAffiPURE gDNA Mini Columns III\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 79.7963%;\"\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.8642%;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer GA:\u003c\/strong\u003e provides a sample enzymatic environment;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eProteinase K:\u003c\/strong\u003e enzymatic hydrolysis of bacterial samples;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eProteinase K Dissolve Solution:\u003c\/strong\u003e Proteinase K Dilution Buffer;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eRNase A:\u003c\/strong\u003e enzymatic hydrolysis of RNA in the sample;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer GB:\u003c\/strong\u003e inactivated Proteinase K, balancing the column;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer PB:\u003c\/strong\u003e removes impurities such as proteins and RNA remaining in DNA;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer PW:\u003c\/strong\u003e removes residual salt ions in DNA;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e elutes the binding DNA on the column;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eAffiPURE gDNA Mini Columns III:\u003c\/strong\u003e adsorbing genomic DNA;\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eCollection Tubes 2mL:\u003c\/strong\u003e filtrate collection tube.\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore RNase A and Proteinase K dry powder at -30°C ~ -15°C. and transport at room temperature. The other components should be stored at room temperature (15°C - 25°C) and transported at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGenomic DNA isolation from \u0026lt; 1.0 x 109 Gram-negative bacteria or Gram-positive bacteria.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdditional Materials Required:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLysozyme \u003cstrong\u003e(AffiGEN, Cat.# AFG-VZ-DE103)\u003c\/strong\u003e (for Gram-positive bacteria), absolute ethanol, 1.5 ml centrifuge tube, water bath or metal bath.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"100 Reactions","offer_id":46855754318155,"sku":"AFG-M-0074","price":130.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE.png?v=1741089473"},{"product_id":"affipure-blood-cell-tissue-bacteria-dna-isolation-mini-kit","title":"AffiPURE® Blood\/Cell\/Tissue\/Bacteria DNA Isolation Mini Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Blood\/Cell\/Tissue\/Bacteria DNA Isolation Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eAffiPURE® Blood\/Cell\/Tissue\/Bacteria DNA Isolation Mini Kit\u003c\/strong\u003e is suitable for extracting genomic DNA from ≤200 μL of fresh or frozen anticoagulated whole blood, \u0026lt;25 mg of animal tissues, \u0026lt;5 × 10^6 of cultured cells and \u0026lt;3 × 10^9 cultured bacterial samples. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol\/chloroform organic solvents or time-consuming alcohol precipitation step. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, enzyme digestion and virus detection.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0004\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 50 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0005\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e200 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer ACL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e48 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer BCL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e48 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WB\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE gDNA Mini Columns II\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003eBuffer ACL:\u003c\/strong\u003e Sample lysis.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eProteinase K:\u003c\/strong\u003e Perform enzymatic lysis of samples.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eBuffer BCL:\u003c\/strong\u003e Perform lysis of samples and provide the binding environment.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eBuffer WA:\u003c\/strong\u003e Remove residual protein contamination.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003eBuffer WB:\u003c\/strong\u003e Remove salt ions.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e Elute the bound DNA.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eAffiPURE gDNA Mini Columns II:\u003c\/strong\u003e Genomic DNA adsorption column.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\n\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Filtrate collection tubes.\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003cbr\u003e\u003c\/strong\u003eStore at 15 ~ 25°C and transport at room temperature. If ambient temperatures often exceed 25°C, we suggest storing Proteinase K at 2 ~ 8°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003eFresh or frozen anticoagulated whole blood without nuclei (≤200 μL).\u003cbr\u003eFresh or frozen anticoagulated whole blood with nuclei (5 - 20 μL).\u003cbr\u003eCultured cells (\u0026lt;5 × 10^6 ).\u003cbr\u003eAnimal tissues (\u0026lt;25 mg).\u003cbr\u003eBacteria (\u0026lt;3 × 10^9 ).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46855769293131,"sku":"AFG-M-0004","price":159.0,"currency_code":"USD","in_stock":true},{"title":"200 Reactions","offer_id":46855792820555,"sku":"AFG-M-0005","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_748a9808-15ba-4847-903f-98140d1afb94.png?v=1741089472"},{"product_id":"affipure-plasmid-mini-kit","title":"AffiPURE® Plasmid Mini Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE®  Plasmid Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cmeta charset=\"utf-8\"\u003eTake 1 - 5 ml of overnight bacteria culture and lyse the cells by an optimized SDS-alkaline method. With this kit, the extraction of multiple samples can be completed within 30 min without involving phenol-chloroform extraction or ethanol precipitation. The kit uses a unique spin column to specifically bind plasmid DNA in the solution in a highly efficient manner under high-salt and low-pH conditions and remove proteins, genomic DNA, RNA, and other impurities to the greatest extent. In the end, pure plasmid DNA is eluted from the silica membrane with a low-salt, high-pH eluent. Each column adsorbs up to 35 µg of plasmid DNA. The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, transfection, and common passaging cells experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cp\u003e\u003cstrong\u003eAFG-M-0075\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e100 Reactions\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P3\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PW1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PW2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE DNA Mini Columns\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRNase A:\u003c\/strong\u003e Degrade RNA in the sample. \u003cbr\u003e\u003cstrong\u003eBuffer P1:\u003c\/strong\u003e Suspend bacteria. Add RNase A to Buffer P1 before the first use. \u003cbr\u003e\u003cstrong\u003eBuffer P2:\u003c\/strong\u003e Lyse bacteria (containing SDS\/NaOH). \u003cbr\u003e\u003cstrong\u003eBuffer P3:\u003c\/strong\u003e Neutralizing buffer. \u003cbr\u003e\u003cstrong\u003eBuffer PW1:\u003c\/strong\u003e Remove proteins and other impurities from plasmids. \u003cbr\u003e\u003cstrong\u003eBuffer PW2:\u003c\/strong\u003e Remove residual salt ions from plasmids. \u003cbr\u003e\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e Elute DNA. \u003cbr\u003e\u003cstrong\u003eAffiPURE DNA Mini Columns:\u003c\/strong\u003e Adsorb plasmid DNA. \u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL: \u003c\/strong\u003eCollect filtrate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cmeta charset=\"utf-8\"\u003eStore at 15 ~ 25°C and transport at room temperature. \u003cbr\u003eIf ambient temperatures often exceed 25°C, we suggest storing RNase A at 2 ~ 8°C. \u003cbr\u003eAfter addition of RNase A, Buffer P1 is stable for 6 months when stored at 2 ~ 8°C. \u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eThis product is is suitable for 1 - 5 ml of overnight bacterial cultures. \u003cbr\u003eWhen preparing low-copy plasmids, scale up the volumes of Buffer P1, Buffer P2, and Buffer P3, and increase the volume of bacterial solution to 5 - 10 ml.  \u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"100 Reactions","offer_id":46855846854987,"sku":"AFG-M-0075","price":100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_ef94567b-03ce-45b5-b0ff-f997fa8df9ed.png?v=1741089470"},{"product_id":"affipure-cell-tissue-total-rna-isolation-kit","title":"AffiPURE®  Cell\/Tissue Total RNA Isolation Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Cell\/Tissue Total RNA Isolation Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003eThe \u003cstrong\u003eAffiPURE® Cell\/Tissue Total RNA Isolation Kit\u003c\/strong\u003e provides a rapid method for extracting total RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology and does not require β-mercaptoethanol, phenol\/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains AffiPURE® gDNA-Filter Columns III which can effectively remove impurities and gDNA. AffiPURE® RNA Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-M-0003\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 50 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e35 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e45 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE gDNA-Filter Columns III (each in a 2 mL Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE RNA Columns III (each in a 2 mL Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free Collection Tubes 1.5 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBuffer RL:\u003c\/strong\u003e Provide the environment required for animal tissue and cell lysis. \u003cbr\u003e\u003cstrong\u003eBuffer RW1:\u003c\/strong\u003e Remove impurities such as proteins and DNA. \u003cbr\u003e\u003cstrong\u003eBuffer RW2:\u003c\/strong\u003e Remove salt ion residues. \u003cbr\u003e\u003cstrong\u003eRNase-free ddH2O:\u003c\/strong\u003e Elute total RNA. \u003cbr\u003e\u003cstrong\u003eAffiPURE gDNA-Filter Columns III:\u003c\/strong\u003e Adsorb DNA and remove impurities in the lysate. \u003cbr\u003e\u003cstrong\u003eAffiPURE RNA Columns III:\u003c\/strong\u003e Adsorb RNA specifically. \u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Collect filtrate. \u003cbr\u003e\u003cstrong\u003eRNase-free Collection Tubes 1.5 mL:\u003c\/strong\u003e Collect RNA. \u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eStorage:\u003cbr\u003e\u003c\/strong\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cmeta charset=\"utf-8\"\u003eStore at 15 ~ 25℃ and transport at room temperature.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003cbr\u003e\u003c\/strong\u003e\u003cmeta charset=\"utf-8\"\u003eAnimal tissues (10 - 20 mg) Cultured cells (\u0026lt;5 × 10^6). \u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46855985693003,"sku":"AFG-M-0003","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_53ee6c0c-8051-416f-8393-9b3c0246fa6b.png?v=1741089469"},{"product_id":"affipure-plant-total-rna-isolation-kit-polysaccharides-polyphenolics-rich","title":"AffiPURE® Plant Total RNA Isolation Kit (Polysaccharides \/ Polyphenolics-Rich)","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Plant Total RNA Isolation Kit (Polysaccharides \/ Polyphenolics-Rich)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cmeta charset=\"utf-8\"\u003eThis kit is suitable for the rapid extraction of total RNA from plant tissues, especially those rich in polysaccharides, polyphenols, or starch (e.g., cotton, mature rice, pine needles,populus, loquat, potato, banana, grape, apple, pear, China rose, buckwheat seeds, and Arabidopsis thaliana seeds), with extraction protocols provided for common plant tissues (e.g., Arabidopsis thaliana and tobacco). The kit is based on silica gel membrane purification technology without time-consuming alcohol precipitation and does not require phenol\/chloroform, or any other toxic reagent during the extraction process. AffiPURE® gDNA-Filter Columns II can effectively remove impurities, adsorbing and removing genomic DNA. AffiPURE® RNA Columns IV can efficiently bind RNA, and with an optimized buffer the obtained total RNA has high purity. The isolated RNA has no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys, Northern Blot, Dot Blot, Poly(A) screening, in vitro translation, RNase protection assay, molecular cloning and other downstream experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0071\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 50 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PRL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PRLPlus\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PRW1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PRW2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE gDNA-Filter Columns II (each in a 2 ml Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE RNA Columns Ⅳ (each in a 2 ml Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free Collection Tubes 1.5 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBuffer PRL:\u003c\/strong\u003e Provide the environment required for plant tissues rich in polysaccharides and polyphenols. \u003cbr\u003e\u003cstrong\u003eBuffer PRLPlus:\u003c\/strong\u003e Provide the environment required for the separation of RNA and DNA (polysaccharide- and polyphenol-rich plants). \u003cbr\u003eProvide the environment required for the lysis of plant tissues and separation of RNA and DNA (common plants). \u003cbr\u003e\u003cstrong\u003eBuffer PRW1:\u003c\/strong\u003e Remove proteins and DNA impurities. Buffer PRW2: Remove residual salt ions. \u003cbr\u003e\u003cstrong\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO:\u003c\/strong\u003e Elute total RNA. \u003cbr\u003e\u003cstrong\u003eAffiPURE gDNA-Filter Columns II:\u003c\/strong\u003e Adsorb DNA and RNA, and remove lysis impurities.  \u003cbr\u003e\u003cstrong\u003eAffiPURE RNA Columns IV:\u003c\/strong\u003e Specifically adsorb RNA. \u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Collect filtrate. \u003cbr\u003e\u003cstrong\u003eRNase-free Collection Tubes 1.5 mL: \u003c\/strong\u003eCollect RNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cmeta charset=\"utf-8\"\u003eStore at 15 ~ 25°C and transport at room temperature.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e50 - 100 mg of plant samples, such as pine needles and wheat leaves. \u003cbr\u003e10 - 20 mg of seed samples, such as red bean seeds and wheat seeds.\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46856007582027,"sku":"AFG-M-0071","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_cc92c75c-f80a-4e31-a3e2-2e0cb0f63d50.png?v=1741089468"},{"product_id":"affipure-gel-dna-extraction-mini-kit","title":"AffiPURE® Gel DNA Extraction Mini Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Gel DNA Extraction Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cmeta charset=\"utf-8\"\u003eThis kit adopts optimized buffer system and silica gel column purification technology, which can recover 70 bp - 20 kb DNA fragments from various concentrations of TAE or TBE agarose gel. DNA adsorption column can specially adsorp DNA under high-salt condition. In addition, the kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by other methods, and remove impurities such as proteins, other organic compounds, inorganic salt ions and oligonucleotide primers. It can ensure that the purification can be completed within 10 - 15 min. The purified DNA can be used directly for ligation, transformation, enzyme digestion, in vitro transcription, PCR, sequencing, microinjection, etc. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0078\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 100 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GDP\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE DNA Mini Columns-G\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBuffer GDP:\u003c\/strong\u003e DNA binding buffer. \u003cbr\u003e\u003cstrong\u003eBuffer GW:\u003c\/strong\u003e Washing buffer; add absolute ethanol by the indicated volume on the bottle before use. \u003cbr\u003e\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e Elution. \u003cbr\u003e\u003cstrong\u003eAffiPURE DNA Mini Columns-G:\u003c\/strong\u003e DNA adsorption columns. \u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Collection tubes for filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cmeta charset=\"utf-8\"\u003eStore at 15 ~ 25℃ and transport at room temperature.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eVarious concentrations of TAE or TBE agarose gel; PCR products, enzymatic reaction systems or other crude DNA products obtained by various methods. Recovered fragments ranged from 70 bp - 20 kb.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif; font-size: 0.875rem;\"\u003e\u003cmeta charset=\"utf-8\"\u003eSelf-prepared Materials :\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cmeta charset=\"utf-8\"\u003e1.5 mL sterilized tubes, absolute ethanol and isopropanol (when DNA fragment ≤100 bp, add 1 volume isopropanol to 1 volume gel), water bath.\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"AffiGEN","offers":[{"title":"100 Reactions","offer_id":46856491401547,"sku":"AFG-M-0078","price":162.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_024fa4fb-f4cd-42af-a3b1-b22ee9efdc55.png?v=1741089467"},{"product_id":"affipure-plant-dna-isolation-mini-kit","title":"AffiPURE® Plant DNA Isolation Mini Kit","description":"\u003ch2\u003e\n\u003cspan style=\"color: #528e90;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Plant DNA Isolation Mini Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003eThis kit is suitable for the rapid extraction of genomic DNA from plant tissues, especially those rich in polysaccharides and polyphenols. The kit is based on silica gel membrane purification technology without time-consuming alcohol precipitation and does not require phenol\/chloroform, or any other toxic reagent during the extraction process, which can effectively remove impurities and other organic compounds from plant cell. The obtained genomic DNA with high purity and stable quality can be directly used in PCR, qPCR and enzyme digestion, etc. The amount of genomic DNA extracted from different plant tissues will vary.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0079\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 50 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e250 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A3\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AW\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPure gDNA Columns IV\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eRNase A:\u003c\/strong\u003e Used to remove RNA.\u003cbr\u003e\u003cstrong\u003eBuffer A1:\u003c\/strong\u003e Provide the lysis enviroment.\u003cbr\u003e\u003cstrong\u003eBuffer A2:\u003c\/strong\u003e Remove protein, cell debris and other impurities.\u003cbr\u003e\u003cstrong\u003eBuffer A3:\u003c\/strong\u003e Provide the binding environment.\u003cbr\u003e\u003cstrong\u003eBuffer AW:\u003c\/strong\u003e Remove residual salt ions of genomic DNA.\u003cbr\u003e\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e Elute genomic DNA.\u003cbr\u003e\u003cstrong\u003eAffiPure gDNA Columns IV:\u003c\/strong\u003e Adsorb genomic DNA.\u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e Collect filtrate. \u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eStore at 15 ~ 25°C and transport at room temperature. If ambient temperatures often exceed 25°C, we suggest storing RNase A at 2 ~ 8°C.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e≤100 mg fresh plant samples.\u003cbr\u003e≤20 mg dried plant samples.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelf-prepared Materials:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eAbsolute ethanol, sterilized 1.5 ml centrifuge tube, mortar, and water bath or thermal shaker (Optional: PVP-40, β-Mercaptoethanol).\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46856527348043,"sku":"AFG-M-0079","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_1e0b773c-621e-45f7-94eb-74221348700a.png?v=1741089466"},{"product_id":"affipure-endofree-plasmid-maxi-kit","title":"AffiPURE® EndoFree Plasmid Maxi Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® EndoFree Plasmid Maxi Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003c\/strong\u003eThis kit is suitable for extraction from 150 - 300 ml of bacterial solution cultured overnight, using an improved SDS-alkaline lysis method to lyse the bacteria. The crude extract is selectively combined with a unique Endotoxin Scavenger and separated by centrifugation to remove endotoxins. Then, the silica gel membrane selectively binds to plasmid DNA in the solution under conditions of high-salt and low-pH. This is followed by addition of wash buffer to remove impurities and other bacterial components. Finally, a low-salt, high-pH elution buffer is used to elute pure plasmid DNA from the silicon matrix membrane. The silica gel membrane employs special adsorption membrane, and the adsorption amount difference between the column and the column is very small and the repeatability is good. Phenol, chloroform and other toxic reagents are not required, and neither is ethanol precipitation steps. This kit can be used to rapidly extract 0.2 - 1.5 mg of pure high-copy plasmid DNA, with an extraction rate of 80% - 90%. The kit uses an unique process formula removes endotoxin, the content of endotoxin is extremely low and the cell transfection effect is excellent. The extracted plasmid could be directly used in enzyme digestion, PCR, in vitro transcription, transformation, sequencing and other molecular biology experiments.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"80%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"20%\"\u003e\n\u003cstrong\u003eAFG-M-0076\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e 10 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e750 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P1\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e75 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e75 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer P4\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e75 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndotoxin Scavenger\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AW\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 22 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TB\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAffiPURE DNA Maxi Columns (each in a 50 ml Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndotoxin-free Collection Tube\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eRNase A:\u003c\/strong\u003e 10 mg\/ml, used to remove RNA.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer P1:\u003c\/strong\u003e bacterial suspension buffer, add RNase A to Buffer P1 before first use.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer P2:\u003c\/strong\u003e bacterial lysis buffer (containing SDS\/NaOH).\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer P4:\u003c\/strong\u003e neutralizing buffer.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eEndotoxin Scavenger:\u003c\/strong\u003e effectively remove endotoxin from the crude plasmid extract.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer PW:\u003c\/strong\u003e wash buffer, add the stipulated volume of ethanol before first use.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBuffer TB:\u003c\/strong\u003e elution buffer.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eAffiPURE DNA Maxi Columns:\u003c\/strong\u003e plasmid DNA adsorption columns.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eCollection Tubes 50 ml:\u003c\/strong\u003e filtrate collection tubes.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eEndotoxin-free Collection Tube:\u003c\/strong\u003e plasmid DNA collection tubes.\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRNase A should be stored at -30 ~ -15°C and transported at ≤0°C. \u003cbr\u003eEndotoxin Scavenger can be stored at 2 ~ 8°C for one month, stored at -30 ~ -15°C for long-term storage and transported at ≤0°C. \u003cbr\u003eOther components should be stored at room temperature (15 ~ 25°C) and transported at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is suitable for large-scale extraction of plasmids from 150 - 300 ml of bacterial solution cultured overnight.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelf-prepared Materials:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAbsolute ethanol, isopropanol, 50 ml round-bottom centrifuge tubes and 50 ml endotoxinfree centrifuge tubes.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"10 Reactions","offer_id":46856565129547,"sku":"AFG-M-0076","price":198.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_55f0dc7d-4b81-4311-8a21-c8c0b90af8a0.png?v=1741089465"},{"product_id":"affipure-blood-dna-isolation-mini-kit-v2","title":"AffiPURE® Blood DNA Isolation Mini Kit V2","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Blood DNA Isolation Mini Kit V2\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp align=\"justify\"\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eAffiPURE® Blood DNA Isolation Mini Kit V2\u003c\/strong\u003e is specially designed for rapid and efficient purification of genomic DNA from 0.1 ml - 1 ml of fresh and frozen whole blood with common anticoagulants. The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for toxic phenol-chloroform extractions, or time-consuming ethanol precipitation. RNA, proteins, Lipids and other impurities such as enzyme inhibitors are removed effectively by the wash buffer. Isolated DNA with high purity and stable quality can be used directly in down-steam applications including restriction enzyme digestion, PCR, Southern blotting.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-VZ-DC111-01\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\n\u003cstrong\u003eAFG-VZ-DC111-02\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e200 Reactions\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eACK Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer BCL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e48 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WB\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFastPure gDNA Mini Columns\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 Columns\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 100 Columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCollection Tubes 2 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 Columns\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 x 100 Columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: \u003c\/strong\u003e \u003cbr\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eACK Lysis Buffer:\u003c\/strong\u003e used for cracking red blood cells in blood samples;\u003cbr\u003e\u003cstrong\u003eProteinase K:\u003c\/strong\u003e used for enzyme digestion of blood samples;\u003cbr\u003e\u003cstrong\u003eBuffer BCL:\u003c\/strong\u003e used for cells lysis and providing binding condition of the DNA to the membrane;\u003cbr\u003e\u003cstrong\u003eBuffer WA:\u003c\/strong\u003e used for the removal of protein residues;\u003cbr\u003e\u003cstrong\u003eBuffer WB:\u003c\/strong\u003e used for the removal of salt ions residues; \u003cbr\u003e\u003cstrong\u003eElution Buffer:\u003c\/strong\u003e used for the elution of genomic DNA;\u003cbr\u003e\u003cstrong\u003eFastPure gDNA Mini Columns:\u003c\/strong\u003e used for adsorbing genomic DNA.\u003cbr\u003e\u003cstrong\u003eCollection Tubes 2 mL:\u003c\/strong\u003e collection tube for filtrate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eAll components except Proteinase K are stored and transported at room temperature (15°C - 25°C). Proteinase K can be stored at 15°C - 25°C for three months (at 2°C - 8°C for long-term), and transported at room temperature. \u003cbr\u003eThe buffer may precipitate when stored at low temperature. Dissolve at room temperature for a while or at 37°C for 10 min to thaw the precipitation and mix thoroughly before use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e0.1 ml - 1 ml of fresh or frozen anticoagulated blood with non-nucleated red blood cells; \u003cbr\u003e5 µl - 20 µl of fresh or frozen anticoagulated blood with nucleated red blood cells.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46859489542475,"sku":"AFG-M-0507","price":86.25,"currency_code":"USD","in_stock":true},{"title":"200 Reactions","offer_id":46859535974731,"sku":"AFG-M-0508","price":319.63,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_d4664746-b14a-4068-9f9f-7b6dda40b788.png?v=1741089463"},{"product_id":"affipure-viral-dna-rna-mini-kit-pro","title":"AffiPURE® Viral DNA\/RNA Mini Kit Pro","description":"\u003ch2\u003e\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® Viral DNA\/RNA Mini Kit Pro\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThis kit is suitable for the rapid extraction of high-purity viral DNA\/RNA from various samples such as blood, serum, plasma, swab, tissue, bronchoalveolar lavage fluid, and cell culture supernatant. Due to the unique lysis system, the kit does not require high temperature incubation, and the addition of Carrier RNA can significantly improve the extraction efficiency of trace nucleic acids. The viral DNA\/RNA can be purified quickly and efficiently by specific adsorption of silica membrane. The kit has a wide range of sample compatibility, and the obtained nucleic acid has a high purity and high yield. That nucleic acid can be directly used in downstream related experiments such as reverse transcription, PCR, fluorescence quantitative PCR, next-generation sequencing and Northern hybridization, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"2\" style=\"width: 70%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e\n\u003cstrong\u003eAFG-M-0089\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e (50 rxns)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 20%;\"\u003e\u003cstrong\u003eBOX 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eCarrier RNA (1 μg\/μl)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e0.6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eProteinase K\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e1.2 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"width: 20%;\"\u003e\u003cstrong\u003eBOX 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eBuffer VL Pro\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e18 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eBuffer VW1 \u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e27 m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eBuffer VW2\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 118px;\"\u003e9 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eRNase-free ddH2O\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eAffiPURE DNA\/RNA Columns (each in a 2 ml Collection Tube)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eRNase-free Collection Tubes 1.5 ml\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 30%;\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eCarrier RNA: Improve the recovery efficiency of trace nucleic acids.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eProteinase K: Digest proteins.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eBuffer VL Pro: Lyse samples and provide nucleic acid binding conditions.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eBuffer VW1: Remove residual proteins and other impurities.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eBuffer VW2: Remove residual salt ions.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eRNase-free ddH2O: Elute DNA\/RNA from adsorption column membrane.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eAffiPURE DNA\/RNA Columns: Specifically adsorb DNA\/RNA.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eCollection Tubes 2 ml: Collect filtrate. RNase-free\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eCollection Tubes 1.5 ml: Collect DNA\/RNA.\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eBOX 1: Store at -30 ~ -15°C and transported at ≤0°C.\u003cbr\u003eBOX 2: Store at 15 ~ 25°C and transported at room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is suitable for viral DNA\/RNA extraction from the following samples.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\"\u003e\u003cstrong\u003eSample Category\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"65%\"\u003e\u003cstrong\u003eSample Category\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBlood\u003c\/td\u003e\n\u003ctd\u003eWhole blood, serum, plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTissue\u003c\/td\u003e\n\u003ctd\u003eLiver, spleen, lung, kidney, lymph, node, small intestine, etc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOthers\u003c\/td\u003e\n\u003ctd\u003eStool, buccal swab, bronchoalveolar lavage fluid, cell culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSelf-prepared Materials\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAbsolute ethanol, RNase-free pipette tips, 1.5 ml RNase-free centrifuge tubes, microcentrifuge tubes, vortex mixer, manual pipettors, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eBefore the first use, please add the specified amount of absolute ethanol to Buffer VW1 and Buffer VW2 according to the label on the bottle, and mark it.\u003c\/li\u003e\n\u003cli\u003eProteinase K in the kit is a cryopreserved component, and should be stored at -30 ~ -15°C after receipt.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe Carrier RNA (1 μg\/μl) in the kit is a cryopreserved component. Store it at -30 ~ -15°C.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ePlease mix it well before use, distribute it into RNase-free centrifuge tubes according to the actual use situation. And avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003eThe samples should be equilibrated to room temperature in advance.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAll procedures, unless otherwise specified, shall be carried out at room temperature.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe virus has a strong ability to infect, and various defense measures must be taken before operation.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freezing and thawing of samples, otherwise the extracted viral DNA\/RNA will be degraded or yield reduced.\u003c\/li\u003e\n\u003cli\u003eWhen using this kit, please wear laboratory clothes, disposable latex gloves, disposable masks, and use RNase-free consumables to avoid RNase contamination to the greatest extent.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":46997289009483,"sku":"AFG-M-0089","price":274.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_3ff7dd71-65fd-4f4e-8e80-d452efb2b7ba.png?v=1741089462"},{"product_id":"affipure-3-flag-tag-peptide-afg-ysn-331","title":"AffiPURE® 3X Flag-tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eFlag polypeptide is a hydrophilic polypeptide encoding 8 amino acids (DYKDDDDK) . It can be used to detect specific anti-Flag monoclonal antibodies. In order to improve the sensitivity of detection, 3×Flag repeated polypeptide (3× flag-tag Peptide) has been developed. This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeated peptides have been widely used to purify Flag fusion proteins and competitively bind Anti-Flag monoclonal antibodies.\n The recommended working concentration of 3×Flag repeated polypeptide is 100-400 μg\/mL.\n \n Features\n \n \u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"4mg","offer_id":47092223476043,"sku":"AFG-YSN-331","price":339.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092223508811,"sku":"AFG-YSN-332","price":780.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_4cf61dea-777e-477c-8e44-d19339ea1da8.png?v=1699871284"},{"product_id":"affipure-flag-tag-peptide-afg-ysn-333","title":"AffiPURE® Flag-tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eFlag polypeptide is a hydrophilic polypeptide encoding 8 amino acids (DYKDDDDK) . It contains an enterokinase restriction site and can be used for immunoaffinity chromatography. Flag peptides can elute proteins under non-denatured conditions and are recognized by a variety of antibodies, such as M1, M2, and M5.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"4mg","offer_id":47092223672651,"sku":"AFG-YSN-333","price":297.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092223738187,"sku":"AFG-YSN-334","price":675.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_f740cda5-6ef2-45d0-b486-94fd55bb404e.png?v=1699871286"},{"product_id":"affipure-c-myc-tag-peptide-afg-ysn-335","title":"AffiPURE® c-Myc Tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003ec-Myc Tag peptide is a synthetic polypeptide. Its amino acid sequence corresponds to the 410-419 sequence of the C-terminal of human c-Myc protein. c-Myc Tag peptides are substitutes for c-Myc fusion proteins. It is used to competitively bind anti-c-Myc antibodies in immunoassay assay. The binding of c-Myc Tag peptides to antibodies is specific; therefore, c-Myc Tag peptides are a mild, ideal reagent for elution of Myc-tag fusion proteins bound to anti-Myc monoclonal antibodies.\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eShipped in a cold pack\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5mg","offer_id":47092224590155,"sku":"AFG-YSN-335","price":297.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092224622923,"sku":"AFG-YSN-336","price":675.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_24899043-2430-453d-b20a-9b0694377514.png?v=1699871289"},{"product_id":"affipure-ha-tag-peptide-afg-ysn-337","title":"AffiPURE® HA Tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eHA Tag Peptide is a synthetic peptide. The amino acid sequence is YPYDVPDYA. The erythrocyte lectin surface antigen determinant derived from influenza virus is identical with the HA amino acid sequence in the HA-Tag fusion protein. Easy to detect by Anti-HA antibody and ELISA. It is used to competitively bind anti-HA antibodies in immunoassay assay. The binding of HA Tag peptides to antibodies is specific; therefore, HA Tag peptides are a mild, ideal reagent for elution of HA fusion proteins bound to anti-HA monoclonal antibodies.\n The recommended working concentration of HA Tag peptides for elution of HA-Tag fusion proteins is 100-400 μg\/mL. \u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5mg","offer_id":47092224819531,"sku":"AFG-YSN-337","price":297.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092224852299,"sku":"AFG-YSN-338","price":675.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_31687d15-6158-4b34-905a-eb74fc475ed6.png?v=1699871291"},{"product_id":"affipure-v5-tag-peptide-afg-ysn-339","title":"AffiPURE® V5 Tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003eV5 Tag Peptide is a synthetic peptide. This is a 14-amino acid polypeptide isolated from the P and V proteins of simian virus 5 (SV5) . It is used to competitively bind anti-V5 antibodies in immunoassay assay. The binding of V5 Tag peptides to antibodies is specific; therefore, V5 Tag peptides are a mild, ideal reagent for elution of V5 fusion proteins bound to anti-V5 monoclonal antibodies.\n The recommended working concentration of V5 Tag peptides for elution of V5-Tag fusion proteins is 100-400 μg\/mL. \u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5mg","offer_id":47092225016139,"sku":"AFG-YSN-339","price":297.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092225048907,"sku":"AFG-YSN-340","price":675.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_6c6819c6-380b-4bea-b174-9e794995e4a6.png?v=1699871294"},{"product_id":"affipure-6-his-tag-peptide-afg-ysn-341","title":"AffiPURE® 6X His Tag Peptide","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/p\u003e\n \u003cp\u003e6×His Tag Peptide is a polypeptide composed of 6 histidine residues with a high selective affinity for nickel ions in the side chain. It is used to competitively bind anti-6×His antibodies in immunoassay assay. The recommended working concentration of 6×His Tag peptides for elution of 6×His-Tag fusion proteins is 100-400 μg\/mL. \u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eShipping Condition: \u003c\/strong\u003eDry ice\u003c\/p\u003e\n \u003cp\u003e\u003cstrong\u003eProduct Format: \u003c\/strong\u003eBox\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"5mg","offer_id":47092225147211,"sku":"AFG-YSN-341","price":465.0,"currency_code":"USD","in_stock":true},{"title":"25mg","offer_id":47092225179979,"sku":"AFG-YSN-342","price":1095.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/products\/AffiPURE_1500x_6245148b-8abf-4219-bed5-77cf7d042819.png?v=1699871296"},{"product_id":"affipure-ffpe-dna-isolation-kit","title":"AffiPURE® FFPE DNA Isolation Kit","description":"\u003ch2\u003e\n\u003cspan style=\"color: #528e90;\"\u003eAffiPURE® FFPE DNA Isolation Kit\u003c\/span\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003c\/h2\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions","offer_id":51825883119947,"sku":"AFG-M-0080","price":179.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiPURE_ba6396b3-6bf5-4dc3-83de-c6f7da226488.png?v=1741089474"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/collections\/AffiPURE.png?v=1722794115","url":"https:\/\/affigen.com\/en-ca\/collections\/affipure.oembed?page=2","provider":"AffiGEN","version":"1.0","type":"link"}