{"title":"AffiCLONE®","description":"","products":[{"product_id":"afficlone-ii-one-step-cloning-kit","title":"AffiCLONE® II One Step Cloning Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiCLONE® II One Step Cloning Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AffiCLONE® technology is a simple, fast, and highly efficient DNA seamless cloning technology. It enables directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward\/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 37°C for 30 min to complete the directional cloning. ClonExpress II is a new generation of recombinant cloning kit. It has a unique ligase-independent system, which significantly reduces the self-ligation background of the vector, and the positive rate can reach more than 95%. Highly optimized reaction buffer and enhanced recombinase Exnase II can significantly improve the recombination efficiency and the tolerance to impurities. These features make it possible to use linearized vectors and inserts directly for recombinant cloning without purification, which significantly simplifies the experimental steps.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #429593;\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\u003cstrong\u003e25 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5X CE II Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExnase II\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e100 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500 bp control insert (20 ng\/μl)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5\u003cspan data-mce-fragment=\"1\"\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e \u003cspan data-mce-fragment=\"1\"\u003e5\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epUC19 control vector，linearized (50 ng\/μl，Amp+)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cspan\u003e5\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cspan\u003e5\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"AffiGEN","offers":[{"title":"25 Reactions (20 µL\/Reaction)","offer_id":46663596441931,"sku":"AFG-M-0144","price":217.0,"currency_code":"USD","in_stock":true},{"title":"50 Reactions (20 µL\/Reaction)","offer_id":46663596474699,"sku":"AFG-M-0145","price":362.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_ddb5099c-7111-4143-87e4-08bc94bdd4f4.png?v=1741089628"},{"product_id":"afficlone-multis-one-step-cloning-kit","title":"AffiCLONE® MultiS One Step Cloning Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiCLONE® MultiS One Step Cloning Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eAffiCLONE®\u003c\/strong\u003e technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward\/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 37℃ for 30 min to complete the directional cloning. ClonExpress MultiS is a new generation of recombinant cloning kit. It is specifically optimized for multi-fragment recombination reactions, enabling up to five inserts to be cloned at one time. This kit has a unique ligase-independent system, which significantly reduces the self-ligation background of the vector, and does not need to consider the restriction endonuclease sites of inserts. Highly optimized reaction buffer and enhanced recombinase Exnase MultiS can significantly improve the recombination efficiency and the tolerance to impurities. These features makes it possible to use linearized vectors and inserts directly for recombinant cloning without purification, which significantly simplifies the experimental steps.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #429593;\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\"\u003e\u003cstrong\u003e25 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5X CE MultiS Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e40 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExnase MultiS\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epUC19 control vector, linearized (50 ng\/μl, Amp+)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControl insert Mix*\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"AffiGEN","offers":[{"title":"10 Reactions (20 µL\/Reaction)","offer_id":46663596507467,"sku":"AFG-M-0146","price":95.0,"currency_code":"USD","in_stock":true},{"title":"25 Reactions (20 µL\/Reaction)","offer_id":46663596540235,"sku":"AFG-M-0147","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_2f4f9a9b-083e-4fb0-b091-93ed164346c4.png?v=1741089627"},{"product_id":"afficlone-clonexpress-ultra-one-step-cloning-kit-v2","title":"AffiCLONE® ClonExpress Ultra One Step Cloning Kit V2","description":"\u003cp\u003eThe AffiCLONE® ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward\/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning. ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFast Cloning\u003c\/li\u003e\n\u003cli\u003eHigh-throughput\u003c\/li\u003e\n\u003cli\u003eCloning Seamless\u003c\/li\u003e\n\u003cli\u003eCloning DNA Site-directed Mutagenesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents\u003c\/td\u003e\n\u003ctd\u003e20 Reactions\u003c\/td\u003e\n\u003ctd\u003e40 Reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2 × CE Mix\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e2 X 100\u003cspan data-mce-fragment=\"1\"\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500 bp control insert (20 ng\/μl)\u003c\/td\u003e\n\u003ctd\u003e5 μL\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epUC19 control vector，linearized (50 ng\/μl，Amp+)\u003c\/td\u003e\n\u003ctd\u003e \u003cspan data-mce-fragment=\"1\"\u003e5 μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"AffiGEN","offers":[{"title":"20 Reactions","offer_id":46663596573003,"sku":"AFG-M-0148","price":218.0,"currency_code":"USD","in_stock":true},{"title":"40 Reactions","offer_id":46664563163467,"sku":"AFG-M-0149","price":348.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_dcd323a3-8287-4222-a78e-be087047fcdc.png?v=1741089626"},{"product_id":"afficlone-ultra-one-step-cloning-kit","title":"AffiCLONE® Ultra One Step Cloning Kit","description":"\u003ch2\u003e\u003cspan style=\"color: #429593;\"\u003eAffiCLONE® Ultra One Step Cloning Kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AffiCLONE® Ultra technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward\/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50℃ for 5 - 15 min to complete the directional cloning. AffiCLONE® Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2X AffiCLONE® Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #429593;\"\u003e\u003cstrong\u003eComponents:\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60%\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003e25 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2X AffiCLONE® Mix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e125 µL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e2 X 125 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epCE-Zero vector, linearized (50 ng\/μl)*\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e25 µL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cspan\u003e50 µL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500bp control insert (20 ng\/μl）\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e5 µL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cspan\u003e5 µL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #429593;\"\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFast Cloning\u003c\/li\u003e\n\u003cli\u003eHigh-throughput\u003c\/li\u003e\n\u003cli\u003eCloning Seamless\u003c\/li\u003e\n\u003cli\u003eCloning DNA Site-directed Mutagenesis\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"AffiGEN","offers":[{"title":"25 Reactions","offer_id":46663596671307,"sku":"AFG-M-0025","price":268.0,"currency_code":"USD","in_stock":true},{"title":"50 Reactions","offer_id":46663596704075,"sku":"AFG-M-0026","price":435.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_a3ac0066-6ba4-4649-8eef-5b428da251e8.png?v=1741089624"},{"product_id":"afficlone-5min-universal-ligation-mix","title":"AffiCLONE® 5 min Universal Ligation Mix","description":"\u003cp\u003e5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt end DNA ligation and TA ligation. Buffer with optimized ligation enhancer make the reaction more efficient and convenient. Ligation can be completed in 5 min at 25℃. The product can be used directly to transform many chemically competent cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003cbr\u003eStore at temperatures between -30℃ and -15℃, and ensure transportation at ≤0℃. After thawing, they can be stored at 4℃ for up to three months, maintaining their quality and efficacy.\u003c\/p\u003e","brand":"AffiGEN","offers":[{"title":"50 Reactions (10 µL\/Reaction)","offer_id":46663597359435,"sku":"AFG-M-0155","price":145.0,"currency_code":"USD","in_stock":true},{"title":"100 Reactions (10 µL\/Reaction)","offer_id":46663597392203,"sku":"AFG-M-0156","price":232.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0754\/5507\/0539\/files\/AffiCLONE_f2050bf1-16d2-4bf4-9a6d-59667b78de8a.png?v=1741089619"},{"product_id":"afficlone-5-min-ta-blunt-zero-cloning-kit","title":"AffiCLONE® 5 min TA\/Blunt-Zero Cloning Kit","description":"\u003cp\u003e5 min TA\/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and a blunt end factor. Combining with the optimal buffer, the second generation of Topoisomerase provides a highly efficient, 5 min, one-step cloning strategy at room temperature. This product using a vector containing the suicide gene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5 min TA\/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt is suitable for ligation of blunt ended and A-Tailed PCR products\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor research use only. 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